Alpaca multiclonal [2F3 / 2E5] Anti-human IgG1/IgG4 antibody (AZdye 568) - FluoTag®-X2

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alpaca multiclonal [2F3 / 2E5] Anti-human IgG1/IgG4 antibody (AZdye 568) - FluoTag®-X2 (AB325307)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized, PBS washing, and blocked with 10% normal goat serum in PBS HeLa (human cervical cancer cells) cells labelling Actin with ab213251 at 1 : 400 (1.20 µg/ml) dilution, followed by ab325307 Alpaca multiclonal [2F3 / 2E5] Anti-human IgG1/IgG4 antibody (AZdye 568) - FluoTag®-X2 at 1/500 (0.15 µg/ml per sdAb) dilution (red).

Confocal image showing cytoplasmic staining in HeLa cells (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a fluorescence microscope (Zeiss Axio Imager Z1).

Secondary antibody only control : Secondary antibody is ab325307 Alpaca multiclonal [2F3 / 2E5] Anti-human IgG1/IgG4 antibody (AZdye 568) - FluoTag®-X2 at 1/500 (0.15 µg/ml per sdAb) dilution.

• Other

Supplier Data

Other - Alpaca multiclonal [2F3 / 2E5] Anti-human IgG1/IgG4 antibody (AZdye 568) - FluoTag®-X2 (AB325307)

Smart Secondary®

Smart Secondary® sdAb generally recognize the Fc domain on the respective primary antibody (the only exception being the FluoTag-X2 anti-Mouse Kappa light chain). Due to the inherent symmetry of the target antibody, two secondary nanobodies will be able to bind each primary antibody. Our secondary FluoTag®-X2 reagents will therefore target 4 fluorophores to each primary antibody. Our anti-Rabbit IgG tools are also available as FluoTag®-X4.

This reagent is a blend of two sdAbs independently binding the same target molecule. As each target site is present twice and each sdAb carries two fluorophores, this reagent can target up to eight fluorophores to each primary antibody – ideal for bright images!