AB6828

Chicken Anti-Rabbit IgG H&L (Biotin)

Name: Chicken Anti Rabbit (IgG) secondary antibody Biotin (ab6828) | Abcam
Brand: Abcam Limited
SKU: AB6828

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(5 Publications)

Suitable for WB, Dot, ELISA, IHC-Fr, IHC-P, IM, ICC/IF. Cited in 5 publications.

1 Images

Supplier Data

Western blot - Chicken Anti-Rabbit IgG H&L (Biotin) (AB6828)

Western blot analysis of ab6828. Secondary antibody : Peroxidase streptavidin secondary antibody at 1/40000 dilution for 30 min at RT.

All lanes:

Western blot - Chicken Anti-Rabbit IgG H&L (Biotin) (ab6828) at 1/1000 dilution

All lanes:

Rabbit IgG at 50 ng

Secondary

All lanes:

Peroxidase streptavidin secondary antibody at 1/40000 dilution

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Key facts

Host species

Chicken

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Biotin

Excitation/Emission

Applications

ICC/IF, IHC-Fr, ELISA, IM, IHC-P, WB, Dot

applications

Clonality

Polyclonal

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000 - 1/10000", "notes":"" }, "Dot": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "ELISA": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IM": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/1000 - 1/5000", "notes":"" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Rabbit IgG

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Anesthesiology 128:784-795 PubMed29346133

2018

Dexmedetomidine Impairs Diaphragm Function and Increases Oxidative Stress but Does Not Aggravate Diaphragmatic Atrophy in Mechanically Ventilated Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas Breuer,Christian Bleilevens,Rolf Rossaint,Gernot Marx,Julian Gehrenkemper,Henning Dierksen,Antoine Delpierre,Joachim Weis,Ghislaine Gayan-Ramirez,Christian S Bruells

The EMBO journal 34:1009-24 PubMed25750208

2015

Human primordial germ cell commitment in vitro associates with a unique PRDM14 expression profile.

Applications

IHC-P

Species

Human

Fumihiro Sugawa,Marcos J Araúzo-Bravo,Juyong Yoon,Kee-Pyo Kim,Shinya Aramaki,Guangming Wu,Martin Stehling,Olympia E Psathaki,Karin Hübner,Hans R Schöler

Journal of dental research 93:1141-7 PubMed25266715

2014

Epithelial TRPV1 signaling accelerates gingival epithelial cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

N Takahashi,Y Matsuda,H Yamada,K Tabeta,T Nakajima,S Murakami,K Yamazaki

Archives of oral biology 57:805-13 PubMed22186069

2011

Bmp signalling in filiform tongue papillae development.

Applications

Unspecified application

Species

Unspecified reactive species

Katsushige Kawasaki,Thantrira Porntaveetus,Shelly Oommen,Sarah Ghafoor,Maiko Kawasaki,Yoko Otsuka-Tanaka,James Blackburn,John A Kessler,Paul T Sharpe,Atsushi Ohazama

BJU international 101:508-12 PubMed18005212

2007

cDNA microarray analysis of differentially expressed genes in penile tissue after treatment with tadalafil.

Applications

Unspecified application

Species

Unspecified reactive species

Enrico Andrade,Priscila Maria Andrade,Ricardo Carneiro Borra,Joaquim Claro,Miguel Srougi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Chicken Anti-Rabbit IgG H&L (Biotin)

Western blot - Chicken Anti-Rabbit IgG H&L (Biotin) (AB6828)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Complementary Products

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (5)

Product promise