AB6825

Chicken Anti-Rabbit IgG H&L (FITC)

Name: Chicken Anti Rabbit (IgG) secondary antibody FITC (ab6825) | Abcam
Brand: Abcam Limited
SKU: AB6825
Price: 265 USD
Availability: InStock

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(4 Publications)

Suitable for Flow Cyt, IHC-Fr, IHC-P, IM, ICC/IF, ELISA, Dot. Cited in 4 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

1 Images

Supplier Data

Dot Blot - Chicken Anti-Rabbit IgG H&L (FITC) (AB6825)

Dot blot analysis of ab6825.

All lanes:

Dot Blot - Chicken Anti-Rabbit IgG H&L (FITC) (ab6825)

Lane 1:

Rabbit IgG at 50 ng

Lane 2:

Rabbit IgG at 16.67 ng

Lane 3:

Rabbit IgG at 5.56 ng

Lane 4:

Rabbit IgG at 1.85 ng

Lane 5:

Rabbit IgG at 0.62 ng

Secondary

All lanes:

Rabbit IgG Antibody Fluorescein Conjugated Antibody at 1/1000 dilution

false

Key facts

Host species

Chicken

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

FITC

Excitation/Emission

Ex: 495nm, Em: 519nm

Applications

ELISA, IM, ICC/IF, IHC-Fr, Dot, Flow Cyt, IHC-P

applications

Clonality

Polyclonal

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Flow Cyt": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IM": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/1000 - 1/5000", "notes":"" }, "ELISA": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/10000 - 1/50000", "notes":"" }, "Dot": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Rabbit IgG

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 21: PubMed33143259

2020

Expression of EMT-Related Genes in Hybrid E/M Colorectal Cancer Cells Determines Fibroblast Activation and Collagen Remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Irina Druzhkova,Marina Shirmanova,Nadezhda Ignatova,Varvara Dudenkova,Maria Lukina,Elena Zagaynova,Dina Safina,Sergey Kostrov,Dmitry Didych,Alexey Kuzmich,George Sharonov,Olga Rakitina,Irina Alekseenko,Eugene Sverdlov

Biomaterials 209:88-102 PubMed31030083

2019

Fibroblasts stimulate macrophage migration in interconnected extracellular matrices through tunnel formation and fiber alignment.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew J Ford,Sophia M Orbach,Padmavathy Rajagopalan

Molecular metabolism 6:48-60 PubMed28123937

2017

Fermentable carbohydrate stimulates FFAR2-dependent colonic PYY cell expansion to increase satiety.

Applications

Unspecified application

Species

Unspecified reactive species

Lucy Brooks,Alexander Viardot,Anastasia Tsakmaki,Emilie Stolarczyk,Jane K Howard,Patrice D Cani,Amandine Everard,Michelle L Sleeth,Arianna Psichas,Jelena Anastasovskaj,Jimmy D Bell,Kim Bell-Anderson,Charles R Mackay,Mohammad A Ghatei,Stephen R Bloom,Gary Frost,Gavin A Bewick

Genome medicine 8:66 PubMed27287041

2016

Extracting a low-dimensional description of multiple gene expression datasets reveals a potential driver for tumor-associated stroma in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Safiye Celik,Benjamin A Logsdon,Stephanie Battle,Charles W Drescher,Mara Rendi,R David Hawkins,Su-In Lee

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Chicken Anti-Rabbit IgG H&L (FITC)

Dot Blot - Chicken Anti-Rabbit IgG H&L (FITC) (AB6825)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (4)

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Product promise