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Suitable for Dot, EM, IHC, WB, ELISA, ICC/IF. Ideal for western blot. Cited in 7 publications.

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Publications

Key facts

Host species
Donkey
Target species
Goat
Target isotype
IgG
Target specificity
Heavy & Light chains
Conjugation
HRP
Applications
Dot, EM, IHC, WB, ELISA, ICC/IF
Clonality
Polyclonal
Format
F(ab)

Reactivity data

Application
Dot
Reactivity
Reacts
Dilution info
-
Notes

-

Application
EM
Reactivity
Reacts
Dilution info
-
Notes

-

Application
IHC
Reactivity
Reacts
Dilution info
-
Notes

-

Application
WB
Reactivity
Reacts
Dilution info
1/2000.00000 - 1/10000.00000
Notes

-

Application
ELISA
Reactivity
Reacts
Dilution info
1/17000
Notes

This product has been assayed against 1.0 µg of Goat IgG in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.

Application
ICC/IF
Reactivity
Reacts
Dilution info
1/500.00000 - 1/2500.00000
Notes

-

Target data

Alternative names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

Recommended products

Suitable for Dot, EM, IHC, WB, ELISA, ICC/IF. Ideal for western blot. Cited in 7 publications.

Key facts

Description
Donkey F(ab) Anti-Goat IgG H&L (HRP)
Host species
Donkey
Target species
Goat
Target isotype
IgG
Target specificity
Heavy & Light chains
Conjugation
HRP
Applications
Dot, EM, IHC, WB, ELISA, ICC/IF
Clonality
Polyclonal
Format
F(ab)
Pre-adsorbed
No
Specificity

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Donkey Serum. No reaction was observed against anti-Papain or anti-Donkey IgG F(c).

Isotype
IgG
Concentration
Loading...

Properties

Form
Liquid
Storage buffer

Preservative: 0.01% Gentamicin sulphate
Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Purification technique
Affinity purification
Purification notes

Fab Goat IgG (H&L) Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

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    Product protocols

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