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AB150140

Donkey F(ab')2 Anti-Goat IgG H&L (Alexa Fluor® 594) preadsorbed

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(2 Publications)

Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 2 publications.
2 Images
Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Goat IgG H&L (Alexa Fluor® 594) preadsorbed (AB150140)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Goat IgG H&L (Alexa Fluor® 594) preadsorbed (AB150140)

ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1μg/ml) overnight at +4°C. ab98800, goat anti-mouse IgG, was then added as a secondary bridging antibody, at 1/250 dilution for 1h. ab150140, Donkey F(ab')2 Anti-Goat IgG H&L (Alexa Fluor® 594) preadsorbed (orange) was then used at 1μg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

The secondary antibody only control displays negative staining, as expected.

Alexa Fluor® - Donkey F(ab')2 Anti-Goat IgG H&L (Alexa Fluor® 594) preadsorbed (AB150140)
  • Alexa Fluor®

Unknown

Alexa Fluor® - Donkey F(ab')2 Anti-Goat IgG H&L (Alexa Fluor® 594) preadsorbed (AB150140)

Key facts

Host species

Donkey

Target species

Goat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat,

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Applications

ELISA, Flow Cyt, IHC-Fr, ICC/IF, IHC-P

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

Reacts with the heavy and light chains of goat IgG as demonstrated by ELISA. May also react with other species and with the light chains of other goat immunoglobulins. Minimal cross reaction to rat and mouse serum proteins.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<p></p>" } } }

Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Gel filtration chromatography of pepsin digested antibody.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

Product protocols

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy : the journal of the American So 27:2123-2133 PubMed31543414

2019

Fetal Gene Therapy Using a Single Injection of Recombinant AAV9 Rescued SMA Phenotype in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Afrooz Rashnonejad,Gholamhossein Amini Chermahini,Cumhur Gündüz,Hüseyin Onay,Ayça Aykut,Burak Durmaz,Meral Baka,Qin Su,Guangping Gao,Ferda Özkınay

Oncology letters 17:1523-1530 PubMed30675208

2019

Evidence for a role of geranylgeranylation in renal angiomyolipoma and renal epithelioid angiomyolipoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dan-Dan Zhao,Jun Yuan,Qi Cheng,Ya-Ling Qi,Ke Lu,Shan-Shan Lai,Qian Sun,Yue Zhao,Lei Fang,Mei-Ling Jin,De-Cai Yu,Yu-Dong Qiu,Chao-Jun Li,Jun Chen,Bin Xue
View all publications

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