Donkey F(ab')2 Anti-Goat IgG H&L (Alexa Fluor® 594) preadsorbed
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(2 Publications)
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Goat IgG H&L (Alexa Fluor® 594) preadsorbed (AB150140)
ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1μg/ml) overnight at +4°C. ab98800, goat anti-mouse IgG, was then added as a secondary bridging antibody, at 1/250 dilution for 1h. ab150140, Donkey F(ab')2 Anti-Goat IgG H&L (Alexa Fluor® 594) preadsorbed (orange) was then used at 1μg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
The secondary antibody only control displays negative staining, as expected.
- Alexa Fluor®
Unknown
Alexa Fluor® - Donkey F(ab')2 Anti-Goat IgG H&L (Alexa Fluor® 594) preadsorbed (AB150140)
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Publications (2)
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Molecular therapy : the journal of the American So 27:2123-2133 PubMed31543414
2019
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Oncology letters 17:1523-1530 PubMed30675208
2019
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