AB181289

Donkey F(ab')2 Anti-Mouse IgG H&L (Alexa Fluor® 488)

Name: Donkey F(ab')2 Anti Mouse (IgG) secondary antibody Alexa Fluor® 488 (ab181289)
Brand: Abcam Limited
SKU: AB181289

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(8 Publications)

Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Cited in 8 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form

2 Images

Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB181289)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB181289)

ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1μg/ml) overnight at +4°C. The secondary antibody (green) was ab181289 Alexa Fluor^® 488 donkey F(ab)2anti-mouse IgG (H &L) used at 2μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

Alexa Fluor® - Donkey F(ab')2 Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB181289)

Alexa Fluor®

Unknown

Alexa Fluor® - Donkey F(ab')2 Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB181289)

Key facts

Host species

Donkey

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Applications

ELISA, Flow Cyt, IHC-Fr, IHC-P, ICC/IF

applications

Clonality

Polyclonal

Isotype

IgG

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/2000", "notes":"" } } }

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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Gel filtration chromatography of pepsin digested antibody.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ighg1

Additional targets

Ighg1

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:2855 PubMed37202403

2023

Autophagy receptor NDP52 alters DNA conformation to modulate RNA polymerase II transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Ália Dos Santos,Daniel E Rollins,Yukti Hari-Gupta,Hannah McArthur,Mingxue Du,Sabrina Yong Zi Ru,Kseniia Pidlisna,Ane Stranger,Faeeza Lorgat,Danielle Lambert,Ian Brown,Kevin Howland,Jesse Aaron,Lin Wang,Peter J I Ellis,Teng-Leong Chew,Marisa Martin-Fernandez,Alice L B Pyne,Christopher P Toseland

The Biochemical journal 479:1409-1428 PubMed35722941

2022

Binding partners regulate unfolding of myosin VI to activate the molecular motor.

Applications

Unspecified application

Species

Unspecified reactive species

Ália Dos Santos,Natalia Fili,Yukti Hari-Gupta,Rosemarie E Gough,Lin Wang,Marisa Martin-Fernandez,Jesse Aaron,Eric Wait,Teng-Leong Chew,Christopher P Toseland

Nature communications 13:1346 PubMed35292632

2022

Myosin VI regulates the spatial organisation of mammalian transcription initiation.

Applications

Unspecified application

Species

Unspecified reactive species

Yukti Hari-Gupta,Natalia Fili,Ália Dos Santos,Alexander W Cook,Rosemarie E Gough,Hannah C W Reed,Lin Wang,Jesse Aaron,Tomas Venit,Eric Wait,Andreas Grosse-Berkenbusch,J Christof M Gebhardt,Piergiorgio Percipalle,Teng-Leong Chew,Marisa Martin-Fernandez,Christopher P Toseland

Nucleic acids research 49:340-353 PubMed33330932

2020

DNA damage alters nuclear mechanics through chromatin reorganization.

Applications

Unspecified application

Species

Unspecified reactive species

Ália Dos Santos,Alexander W Cook,Rosemarie E Gough,Martin Schilling,Nora A Olszok,Ian Brown,Lin Wang,Jesse Aaron,Marisa L Martin-Fernandez,Florian Rehfeldt,Christopher P Toseland

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 35:e21167 PubMed33241563

2020

Staphylococcus aureus Panton-Valentine Leukocidin triggers an alternative NETosis process targeting mitochondria.

Applications

Unspecified application

Species

Unspecified reactive species

Viola Mazzoleni,Kiran Zimmermann,Anna Smirnova,Ivan Tarassov,Gilles Prévost

Neural regeneration research 15:1290-1299 PubMed31960815

2020

No DCX-positive neurogenesis in the cerebral cortex of the adult primate.

Applications

Unspecified application

Species

Unspecified reactive species

Ruo-Xu Liu,Jie Ma,Bin Wang,Tian Tian,Ning Guo,Shao-Jun Liu

The Journal of biological chemistry 295:337-347 PubMed31744880

2019

Competition between two high- and low-affinity protein-binding sites in myosin VI controls its cellular function.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia Fili,Yukti Hari-Gupta,Bjork Aston,Ália Dos Santos,Rosemarie E Gough,Bana Alamad,Lin Wang,Marisa L Martin-Fernandez,Christopher P Toseland

Frontiers in cellular and infection microbiology 7:157 PubMed28536674

2017

Anti-Idiotypic Antibodies Specific to prM Monoantibody Prevent Antibody Dependent Enhancement of Dengue Virus Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Miao Wang,Fan Yang,Dana Huang,Yalan Huang,Xiaomin Zhang,Chao Wang,Shaohua Zhang,Renli Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey F(ab')2 Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB181289)

Alexa Fluor® - Donkey F(ab')2 Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB181289)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Additional targets

Publications (8)

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