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AB181292

Donkey F(ab')2 Anti-Mouse IgG H&L (Alexa Fluor® 647)

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(4 Publications)

Suitable for ELISA, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Cited in 4 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-4, Ighg1

2 Images
Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Mouse IgG H&L (Alexa Fluor® 647) (AB181292)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Mouse IgG H&L (Alexa Fluor® 647) (AB181292)

ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1μg/ml) overnight at +4°C. The secondary antibody (red) was ab181292 Alexa Fluor® 647 donkey F(ab)2 anti-mouse IgG (H+L) used at 2μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

Alexa Fluor® - Donkey F(ab')2 Anti-Mouse IgG H&L (Alexa Fluor® 647) (AB181292)
  • Alexa Fluor®

Unknown

Alexa Fluor® - Donkey F(ab')2 Anti-Mouse IgG H&L (Alexa Fluor® 647) (AB181292)

Key facts

Host species

Donkey

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Applications

IHC-P, Flow Cyt, ELISA, ICC/IF

applications

Clonality

Polyclonal

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/2000", "notes":"<p></p>" } } }

Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Gel filtration chromatography of pepsin digested antibody.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

Product protocols

Target data

See full target information Ighg1

Additional targets

Ighg1

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:112651 PubMed40510119

2025

A calcium-sensing receptor dileucine motif directs internalization to spatially distinct endosomal signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Rachael A Wyatt,Meurig T Gallagher,Ling Zha,Christopher J McCabe,Caroline M Gorvin

The Journal of physiology 602:5061-5081 PubMed39231098

2024

PAK1 inhibition with Romidepsin attenuates H-reflex hyperexcitability after spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Sierra D Kauer,Curtis A Benson,Jennifer M Carrara,Afrin A Tarafder,Youssef H Ibrahim,Maile A Estacion,Stephen G Waxman,Andrew M Tan

The Journal of neuroscience : the official journal of the Society for Neuroscience 42:5361-5372 PubMed35610049

2022

Selective Inhibitory Circuit Dysfunction after Chronic Frontal Lobe Contusion.

Applications

Unspecified application

Species

Unspecified reactive species

Amber L Nolan,Vikaas S Sohal,Susanna Rosi

Bio-protocol 10:e3783 PubMed33659438

2020

Multitarget Immunohistochemistry for Confocal and Super-resolution Imaging of Plant Cell Wall Polysaccharides.

Applications

Unspecified application

Species

Unspecified reactive species

Kalina T Haas,Methieu Rivière,Raymond Wightman,Alexis Peaucelle
View all publications

Product promise

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