Donkey F(ab')2 Anti-Mouse IgG H&L (Alexa Fluor® 647)
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(4 Publications)
Suitable for ELISA, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Cited in 4 publications.
View Alternative Names
Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-4, Ighg1
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Mouse IgG H&L (Alexa Fluor® 647) (AB181292)
ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1μg/ml) overnight at +4°C. The secondary antibody (red) was ab181292 Alexa Fluor® 647 donkey F(ab)2 anti-mouse IgG (H+L) used at 2μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
- Alexa Fluor®
Unknown
Alexa Fluor® - Donkey F(ab')2 Anti-Mouse IgG H&L (Alexa Fluor® 647) (AB181292)
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Publications (4)
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iScience 28:112651 PubMed40510119
2025
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The Journal of physiology 602:5061-5081 PubMed39231098
2024
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The Journal of neuroscience : the official journal of the Society for Neuroscience 42:5361-5372 PubMed35610049
2022
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Bio-protocol 10:e3783 PubMed33659438
2020
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