AB181346

Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed

Name: Donkey F(ab')2 Anti Rabbit (IgG) secondary antibody preadsorbed Alexa Fluor® 488
Brand: Abcam Limited
SKU: AB181346
Price: 240 USD
Availability: InStock

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(12 Publications)

Suitable for ELISA, IHC-Fr, IHC-P, IF, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 12 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

2 Images

Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (AB181346)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (AB181346)

ICC/IF image of ab6046 in HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1μg/ml) overnight at +4°C. The secondary antibody ab181346 (shown in green) was used at 1μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

Alexa Fluor® - Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (AB181346)

Alexa Fluor®

Unknown

Alexa Fluor® - Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (AB181346)

Key facts

Host species

Donkey

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Human

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Applications

IHC-Fr, IF, ELISA, Flow Cyt, ICC/IF, IHC-P

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

Reacts with the heavy and light chains of rabbit IgG as demonstrated by ELISA; may also react with other species and with the light chains of other rabbit immunoglobulins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1 µg/mL", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"" } } }

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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Gel filtration chromatography of pepsin digested antibody.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Rabbit IgG

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Journal of biomedical materials research. Part B, Applied biomaterials 111:16-25 PubMed35833260

2022

The significance of nanofiber polyglycolic acid for promoting tissue repair in a rat subcutaneous implantation model.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Sueyoshi,Atsuko Niwa,Yuki Nishikawa,Noritaka Isogai

Nature communications 13:1346 PubMed35292632

2022

Myosin VI regulates the spatial organisation of mammalian transcription initiation.

Applications

Unspecified application

Species

Unspecified reactive species

Yukti Hari-Gupta,Natalia Fili,Ália Dos Santos,Alexander W Cook,Rosemarie E Gough,Hannah C W Reed,Lin Wang,Jesse Aaron,Tomas Venit,Eric Wait,Andreas Grosse-Berkenbusch,J Christof M Gebhardt,Piergiorgio Percipalle,Teng-Leong Chew,Marisa Martin-Fernandez,Christopher P Toseland

Journal for immunotherapy of cancer 10: PubMed35140112

2022

PAD-2-mediated citrullination of nucleophosmin provides an effective target for tumor immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Ruhul H Choudhury,Peter Symonds,Samantha J Paston,Ian Daniels,Katherine W Cook,Mohamed Gijon,Rachael L Metheringham,Victoria A Brentville,Lindy G Durrant

Frontiers in endocrinology 12:745984 PubMed34630335

2021

Glucose Metabolism Disorder Induces Spermatogenic Dysfunction in Northern Pig-Tailed Macaques () With Long-Term SIVmac239 Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Tian-Zhang Song,Ming-Xu Zhang,Han-Dan Zhang,Xue-Hui Wang,Wei Pang,Ren-Rong Tian,Yong-Tang Zheng

Biomolecules 11: PubMed33672015

2021

A Targeted and Tuneable DNA Damage Tool Using CRISPR/Cas9.

Applications

Unspecified application

Species

Unspecified reactive species

Ioannis Emmanouilidis,Natalia Fili,Alexander W Cook,Yukti Hari-Gupta,Ália Dos Santos,Lin Wang,Marisa L Martin-Fernandez,Peter J I Ellis,Christopher P Toseland

The Journal of physiology 599:1115-1130 PubMed33347681

2021

Contribution of retrotrapezoid nucleus neurons to CO -amplified cardiorespiratory activity in spontaneously hypertensive rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yanming Tian,Danyang Geng,Yakun Wang,Luo Shi,Hongxiao Yu,Wei He,Yufang Zhu,Shirui Jun,Congrui Fu,Xin Wang,Xiangjian Zhang,Fang Yuan,Sheng Wang

Nucleic acids research 49:340-353 PubMed33330932

2020

DNA damage alters nuclear mechanics through chromatin reorganization.

Applications

Unspecified application

Species

Unspecified reactive species

Ália Dos Santos,Alexander W Cook,Rosemarie E Gough,Martin Schilling,Nora A Olszok,Ian Brown,Lin Wang,Jesse Aaron,Marisa L Martin-Fernandez,Florian Rehfeldt,Christopher P Toseland

Cancer communications (London, England) 40:518-530 PubMed32888253

2020

Proteomics identifies EGF-like domain multiple 7 as a potential therapeutic target for epidermal growth factor receptor-positive glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Fei-Yi-Fan Wang,Si-Yi Wang-Gou,Hang Cao,Nian Jiang,Qi Yang,Qi Huang,Chun-Hai Huang,Xue-Jun Li

Neural regeneration research 15:1290-1299 PubMed31960815

2020

No DCX-positive neurogenesis in the cerebral cortex of the adult primate.

Applications

Unspecified application

Species

Unspecified reactive species

Ruo-Xu Liu,Jie Ma,Bin Wang,Tian Tian,Ning Guo,Shao-Jun Liu

The Journal of biological chemistry 295:337-347 PubMed31744880

2019

Competition between two high- and low-affinity protein-binding sites in myosin VI controls its cellular function.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia Fili,Yukti Hari-Gupta,Bjork Aston,Ália Dos Santos,Rosemarie E Gough,Bana Alamad,Lin Wang,Marisa L Martin-Fernandez,Christopher P Toseland

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed

Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (AB181346)

Alexa Fluor® - Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (AB181346)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (12)

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