AB150070

Donkey F(ab')2 Anti-Rabbit IgG - H&L (Alexa Fluor® 555), pre-adsorbed

Name: Donkey F(ab')2 Anti-Rabbit IgG - (Alexa Fluor® 555), pre-adsorbed secondary antibody (ab150070) | Abcam
Brand: Abcam Limited
SKU: AB150070
Price: 205 USD
Availability: InStock

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(7 Publications)

Suitable for IHC-Fr, ELISA, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 7 publications.

2 Images

Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Rabbit IgG - H&L (Alexa Fluor® 555), pre-adsorbed (AB150070)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Rabbit IgG - H&L (Alexa Fluor® 555), pre-adsorbed (AB150070)

ICC/IF image of ab6046 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1μg/ml) overnight at +4°C. The secondary antibody (yellow) was ab150070, Donkey F(ab')2 Anti-Rabbit IgG - H&L (Alexa Fluor® 555), pre-adsorbed, used at 2μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

The secondary antibody only control displays negative staining, as expected.

Alexa Fluor® - Donkey F(ab')2 Anti-Rabbit IgG - H&L (Alexa Fluor® 555), pre-adsorbed (AB150070)

Alexa Fluor®

Unknown

Alexa Fluor® - Donkey F(ab')2 Anti-Rabbit IgG - H&L (Alexa Fluor® 555), pre-adsorbed (AB150070)

Key facts

Host species

Donkey

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Human

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Applications

ICC/IF, IHC-Fr, IHC-P, Flow Cyt, ELISA

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

Reacts with the heavy and light chains of rabbit IgG as demonstrated by ELISA; may also react with other species and with the light chains of other rabbit immunoglobulins

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"Use at an assay dependent dilution" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"Use at an assay dependent dilution" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"" } } }

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Product details

This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, rat and sheep immunosorbents to remove cross reactive antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: 68% PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Rabbit IgG

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Nucleic acids 31:596-609 PubMed36910709

2023

base editing rescues photoreceptors in a mouse model of retinitis pigmentosa.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Su,Kaiqin She,Li Song,Xiu Jin,Ruiting Li,Qinyu Zhao,Jianlu Xiao,Danian Chen,Hui Cheng,Fang Lu,Yuquan Wei,Yang Yang

Frontiers in endocrinology 12:745984 PubMed34630335

2021

Glucose Metabolism Disorder Induces Spermatogenic Dysfunction in Northern Pig-Tailed Macaques () With Long-Term SIVmac239 Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Tian-Zhang Song,Ming-Xu Zhang,Han-Dan Zhang,Xue-Hui Wang,Wei Pang,Ren-Rong Tian,Yong-Tang Zheng

The Journal of biological chemistry 294:14454-14466 PubMed31337707

2019

ANKRD9 is a metabolically-controlled regulator of IMPDH2 abundance and macro-assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Dawn Hayward,Valentina L Kouznetsova,Hannah E Pierson,Nesrin M Hasan,Estefany R Guzman,Igor F Tsigelny,Svetlana Lutsenko

Cell and tissue research 378:457-469 PubMed31309318

2019

Distribution and co-expression patterns of specific cell markers of enteroendocrine cells in pig gastric epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Linda J Fothergill,Giorgia Galiazzo,Billie Hunne,Martin J Stebbing,Josiane Fakhry,Frank Weissenborn,Therese E Fazio Coles,John B Furness

FEBS open bio 9:206-225 PubMed30761248

2019

Recurrent activations of transient receptor potential vanilloid-1 and vanilloid-4 promote cellular proliferation and migration in esophageal squamous cell carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rongqi Huang,Fei Wang,Yuchen Yang,Wenbo Ma,Zuoxian Lin,Na Cheng,Yan Long,Sihao Deng,Zhiyuan Li

Pathology international 67:350-354 PubMed28618063

2017

Papillary thyroid carcinoma expressing CD20.

Applications

Unspecified application

Species

Unspecified reactive species

Yuya Sasaki,Masao Seto,Hiroaki Miyoshi,Shinya Okada,Shintaro Yokoyama,Akira Ohshima,Koichi Ohshima

Cancer medicine 5:3235-3245 PubMed27709813

2016

Expression of Tim-1 in primary CNS lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wataru Kishimoto,Momoko Nishikori,Hiroshi Arima,Hiroaki Miyoshi,Yuya Sasaki,Toshio Kitawaki,Kotaro Shirakawa,Takeharu Kato,Yoshitaka Imaizumi,Takayuki Ishikawa,Hitoshi Ohno,Hironori Haga,Koichi Ohshima,Akifumi Takaori-Kondo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey F(ab')2 Anti-Rabbit IgG - H&L (Alexa Fluor® 555), pre-adsorbed

Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Rabbit IgG - H&L (Alexa Fluor® 555), pre-adsorbed (AB150070)

Alexa Fluor® - Donkey F(ab')2 Anti-Rabbit IgG - H&L (Alexa Fluor® 555), pre-adsorbed (AB150070)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (7)

Product promise