Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 23 publications.
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- Research (Washington, D.C.) 6:00452023Efficient Modulation of Exon Skipping via Antisense Circular RNAs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuaiwei Ren,Mei Huang,Raoxian Bai,Lijiao Chen,Jiao Yang,Junyu Zhang,Wenting Guo,Weizhi Ji,Yongchang ChenPubMed 37040525
- Cell death and differentiation 29:1719-17292022ENKD1 promotes epidermal stratification by regulating spindle orientation in basal keratinocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTao Zhong,Xiaofan Wu,Wei Xie,Xiangrui Luo,Ting Song,Shuang Sun,Youguang Luo,Dengwen Li,Min Liu,Songbo Xie,Jun ZhouPubMed 35197565
- Nature protocols 17:890-9092022Spotting-based differentiation of functional dopaminergic progenitors from human pluripotent stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJisun Kim,Jeha Jeon,Bin Song,Nayeon Lee,Sanghyeok Ko,Young Cha,Pierre Leblanc,Hyemyung Seo,Kwang-Soo KimPubMed 35140411
- Frontiers in cellular neuroscience 15:7336072021Differential Response of Hippocampal and Cerebrocortical Autophagy and Ketone Body Metabolism to the Ketogenic Diet.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniela Liśkiewicz,Arkadiusz Liśkiewicz,Marta M Nowacka-Chmielewska,Mateusz Grabowski,Natalia Pondel,Konstancja Grabowska,Sebastian Student,Jaroslaw J Barski,Andrzej MałeckiPubMed 34456688
- Genes 11:2020Leukocyte Nucleolus and -When Falling Apart Means Falling in Place.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIvona Mladineo et. al.PubMed 32585969
- Scientific reports 10:97052020Increased expression of PD-1 and PD-L1 in oral lesions progressing to oral squamous cell carcinoma: a pilot study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKanan Dave et. al.PubMed 32546692
- Cancer immunology research 8:530-5432020γδ T-cell Receptors Derived from Breast Cancer-Infiltrating T Lymphocytes Mediate Antitumor Reactivity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnke Janssen et. al.PubMed 32019779
- International journal of molecular sciences 20:2019Analysis of Expression and Functional Activity of Aromatic L-Amino Acid Decarboxylase (DDC) and Serotonin Transporter (SERT) as Potential Sources of Serotonin in Mouse Ovary.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDenis A Nikishin,Nina M Alyoshina,Maria L Semenova,Yuri B ShmuklerPubMed 31234589
- The Plant journal : for cell and molecular biology 98:359-3692019sRNA-FISH: versatile fluorescent in situ detection of small RNAs in plants.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKun Huang et. al.PubMed 30577085
- Biological procedures online 20:202018Three-Dimensional Quantification of Spheroid Degradation-Dependent Invasion and Invadopodia Formation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCameron Goertzen et. al.PubMed 30356830
Key facts
- Host species
- Donkey
- Target species
- Rabbit
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Rat, Human
- Conjugation
- Alexa Fluor® 568
- Excitation/Emission
- Ex: 578nm, Em: 603nm
- Applications
- ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt
- Clonality
- Polyclonal
- Format
- F(ab')2
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000 | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS
Recommended products
Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 23 publications.
Key facts
- Description
- Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 568) preadsorbed
- Host species
- Donkey
- Target species
- Rabbit
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Rat, Human
- Conjugation
- Alexa Fluor® 568
- Excitation/Emission
- Ex: 578nm, Em: 603nm
- Applications
- ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt
- Clonality
- Polyclonal
- Format
- F(ab')2
- Pre-adsorbed
- Yes
- Specificity
Reacts with the heavy and light chains of rabbit IgG as demonstrated by ELISA; may also react with other species and with the light chains of other rabbit immunoglobulins.
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
Gel filtration chromatography of pepsin digested antibody
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 568) preadsorbed (ab175694)
ICC/IF image of Anti-beta Tubulin antibody - Loading Control ab6046 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-beta Tubulin antibody - Loading Control ab6046, 1μg/ml) overnight at +4°C. The secondary antibody (yellow) was ab175694 Alexa Fluor® 568 donkey F(ab')2 anti-rabbit IgG (H+L) used at 2μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
Alexa Fluor® 568 is pseudocolored in yellow.
Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 568) preadsorbed (ab175694)
HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (yellow) was ab175694 Alexa Fluor® 568 donkey F(ab')2 anti-rabbit IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Alexa Fluor® 568 is pseudocolored in yellow.
Alexa Fluor® - Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 568) preadsorbed (ab175694)
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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