AB150072

Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed

Name: Donkey F(ab')2 Anti Rabbit (IgG) secondary antibody preadsorbed Alexa Fluor® 594
Brand: Abcam Limited
SKU: AB150072
Price: 205 USD
Availability: InStock

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(11 Publications)

Suitable for IHC-Fr, ICC/IF, IHC-P, Flow Cyt, ELISA. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 11 publications.

2 Images

Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (AB150072)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (AB150072)

ICC/IF image of ab6046 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 1μg/ml) overnight at +4°C. The secondary antibody (orange) was ab150072, Donkey F(ab')2 Anti-Rabbit IgG - H&L (Alexa Fluor® 594), pre-adsorbed, used at 2μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

The secondary antibody only control displays negative staining, as expected.

Alexa Fluor® - Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (AB150072)

Alexa Fluor®

Unknown

Alexa Fluor® - Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (AB150072)

Key facts

Host species

Donkey

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Human

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Applications

ELISA, Flow Cyt, ICC/IF, IHC-P, IHC-Fr

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

Reacts with the heavy and light chains of rabbit IgG as demonstrated by ELISA; may also react with other species and with the light chains of other rabbit immunoglobulins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"Use at an assay dependent dilution" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"Use at an assay dependent dilution" } } }

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Product details

This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, rat and sheep immunosorbents to remove cross reactive antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Rabbit IgG

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 13: PubMed40722781

2025

Evaluation of Apoptosis and Cytotoxicity Induction Using a Recombinant Newcastle Disease Virus Expressing Human IFN-γ in Human Prostate Cancer Cells In Vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Aldo Rojas-Neyra,Katherine Calderón,Brigith Carbajal-Lévano,Gloria Guerrero-Fonseca,Gisela Isasi-Rivas,Ana Chumbe,Ray W Izquierdo-Lara,Astrid Poma-Acevedo,Freddy Ygnacio,Dora Rios-Matos,Manolo Fernández-Sánchez,Manolo Fernández-Díaz

Cellular immunology 403-404:104860 PubMed39084187

2024

R406 reduces lipopolysaccharide-induced neutrophil activation.

Applications

Unspecified application

Species

Unspecified reactive species

Seth Warner,Heather L Teague,Marcos J Ramos-Benitez,Sumith Panicker,Kiana Allen,Salina Gairhe,Tom Moyer,Bindu Parachalil Gopalan,Iyadh Douagi,Arun Shet,Yogendra Kanthi,Anthony F Suffredini,Daniel S Chertow,Jeffrey R Strich

Nature communications 15:796 PubMed38280858

2024

Sexual dimorphism in melanocyte stem cell behavior reveals combinational therapeutic strategies for cutaneous repigmentation.

Applications

Unspecified application

Species

Unspecified reactive species

Luye An,Dahihm Kim,Leanne R Donahue,Menansili Abraham Mejooli,Chi-Yong Eom,Nozomi Nishimura,Andrew C White

The EMBO journal 42:e115076 PubMed37987153

2023

Centriole elimination during Caenorhabditis elegans oogenesis initiates with loss of the central tube protein SAS-1.

Applications

Unspecified application

Species

Unspecified reactive species

Marie Pierron,Alexander Woglar,Coralie Busso,Keshav Jha,Tamara Mikeladze-Dvali,Marie Croisier,Pierre Gönczy

PLoS biology 20:e3001784 PubMed36107993

2022

Molecular architecture of the C. elegans centriole.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander Woglar,Marie Pierron,Fabian Zacharias Schneider,Keshav Jha,Coralie Busso,Pierre Gönczy

eLife 10: PubMed33491649

2021

TRIM37 prevents formation of centriolar protein assemblies by regulating Centrobin.

Applications

Unspecified application

Species

Unspecified reactive species

Fernando R Balestra,Andrés Domínguez-Calvo,Benita Wolf,Coralie Busso,Alizée Buff,Tessa Averink,Marita Lipsanen-Nyman,Pablo Huertas,Rosa M Ríos,Pierre Gönczy

Neural regeneration research 15:1290-1299 PubMed31960815

2020

No DCX-positive neurogenesis in the cerebral cortex of the adult primate.

Applications

Unspecified application

Species

Unspecified reactive species

Ruo-Xu Liu,Jie Ma,Bin Wang,Tian Tian,Ning Guo,Shao-Jun Liu

The FEBS journal 286:4597-4620 PubMed31276291

2019

A novel cell-based sensor detecting the activity of individual basic proprotein convertases.

Applications

Unspecified application

Species

Unspecified reactive species

Karin Löw,Kornelia Hardes,Chiara Fedeli,Nabil G Seidah,Daniel B Constam,Antonella Pasquato,Torsten Steinmetzer,Alexandre Roulin,Stefan Kunz

The Journal of experimental medicine 214:623-637 PubMed28148688

2017

mutations cause skeletal dysplasia, immune deficiency, and developmental delay.

Applications

Unspecified application

Species

Unspecified reactive species

Stefano Volpi,Yasuhiro Yamazaki,Patrick M Brauer,Ellen van Rooijen,Atsuko Hayashida,Anne Slavotinek,Hye Sun Kuehn,Maja Di Rocco,Carlo Rivolta,Ileana Bortolomai,Likun Du,Kerstin Felgentreff,Lisa Ott de Bruin,Kazutaka Hayashida,George Freedman,Genni Enza Marcovecchio,Kelly Capuder,Prisni Rath,Nicole Luche,Elliott J Hagedorn,Antonella Buoncompagni,Beryl Royer-Bertrand,Silvia Giliani,Pietro Luigi Poliani,Luisa Imberti,Kerry Dobbs,Fabienne E Poulain,Alberto Martini,John Manis,Robert J Linhardt,Marita Bosticardo,Sergio Damian Rosenzweig,Hane Lee,Jennifer M Puck,Juan Carlos Zúñiga-Pflücker,Leonard Zon,Pyong Woo Park,Andrea Superti-Furga,Luigi D Notarangelo

The Journal of general physiology 148:341-55 PubMed27670899

2016

Simultaneous imaging of local calcium and single sarcomere length in rat neonatal cardiomyocytes using yellow Cameleon-Nano140.

Applications

ICC/IF

Species

Unspecified reactive species

Seiichi Tsukamoto,Teruyuki Fujii,Kotaro Oyama,Seine A Shintani,Togo Shimozawa,Fuyu Kobirumaki-Shimozawa,Shin'ichi Ishiwata,Norio Fukuda

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed

Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (AB150072)

Alexa Fluor® - Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (AB150072)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (11)

Product promise