Suitable for IHC-Fr, ICC/IF, IHC-P, Flow Cyt, ELISA. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 8 publications.
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Publications
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- The EMBO journal 42:e1150762023Centriole elimination during Caenorhabditis elegans oogenesis initiates with loss of the central tube protein SAS-1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarie Pierron,Alexander Woglar,Coralie Busso,Keshav Jha,Tamara Mikeladze-Dvali,Marie Croisier,Pierre GönczyPubMed 37987153
- PLoS biology 20:e30017842022Molecular architecture of the C. elegans centriole.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexander Woglar,Marie Pierron,Fabian Zacharias Schneider,Keshav Jha,Coralie Busso,Pierre GönczyPubMed 36107993
- eLife 10:2021TRIM37 prevents formation of centriolar protein assemblies by regulating Centrobin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFernando R Balestra,Andrés Domínguez-Calvo,Benita Wolf,Coralie Busso,Alizée Buff,Tessa Averink,Marita Lipsanen-Nyman,Pablo Huertas,Rosa M Ríos,Pierre GönczyPubMed 33491649
- Neural regeneration research 15:1290-12992020No DCX-positive neurogenesis in the cerebral cortex of the adult primate.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuo-Xu Liu,Jie Ma,Bin Wang,Tian Tian,Ning Guo,Shao-Jun LiuPubMed 31960815
- The FEBS journal 286:4597-46202019A novel cell-based sensor detecting the activity of individual basic proprotein convertases.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKarin Löw,Kornelia Hardes,Chiara Fedeli,Nabil G Seidah,Daniel B Constam,Antonella Pasquato,Torsten Steinmetzer,Alexandre Roulin,Stefan KunzPubMed 31276291
- The Journal of experimental medicine 214:623-6372017mutations cause skeletal dysplasia, immune deficiency, and developmental delay.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStefano Volpi et. al.PubMed 28148688
- The Journal of general physiology 148:341-552016Simultaneous imaging of local calcium and single sarcomere length in rat neonatal cardiomyocytes using yellow Cameleon-Nano140.Applications:ICC/IFReactive species:Unspecified reactive speciesSeiichi Tsukamoto,Teruyuki Fujii,Kotaro Oyama,Seine A Shintani,Togo Shimozawa,Fuyu Kobirumaki-Shimozawa,Shin'ichi Ishiwata,Norio FukudaPubMed 27670899
- Nature biotechnology 34:973-812016Multiplexed and scalable super-resolution imaging of three-dimensional protein localization in size-adjustable tissues.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTaeyun Ku et. al.PubMed 27454740
Key facts
- Host species
- Donkey
- Target species
- Rabbit
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Rat, Human
- Conjugation
- Alexa Fluor® 594
- Excitation/Emission
- Ex: 590nm, Em: 617nm
- Applications
- IHC-Fr, ICC/IF, IHC-P, Flow Cyt, ELISA
- Clonality
- Polyclonal
- Format
- F(ab')2
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes Use at an assay dependent dilution |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000 | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes Use at an assay dependent dilution |
Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS
Recommended products
Suitable for IHC-Fr, ICC/IF, IHC-P, Flow Cyt, ELISA. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 8 publications.
Key facts
- Description
- Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed
- Host species
- Donkey
- Target species
- Rabbit
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Rat, Human
- Conjugation
- Alexa Fluor® 594
- Excitation/Emission
- Ex: 590nm, Em: 617nm
- Applications
- IHC-Fr, ICC/IF, IHC-P, Flow Cyt, ELISA
- Clonality
- Polyclonal
- Format
- F(ab')2
- Pre-adsorbed
- Yes
- Specificity
Reacts with the heavy and light chains of rabbit IgG as demonstrated by ELISA; may also react with other species and with the light chains of other rabbit immunoglobulins.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
Antiserum was cross adsorbed using bovine, chicken, goat, human, mouse, rat and sheep immunosorbents to remove cross reactive antibodies. The antibody to rabbit IgG was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Product promise
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Terms & Conditions.
2 product images
Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150072)
ICC/IF image of Anti-beta Tubulin antibody - Loading Control ab6046 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-beta Tubulin antibody - Loading Control ab6046, 1μg/ml) overnight at +4°C. The secondary antibody (orange) was ab150072, Donkey F(ab')2 Anti-Rabbit IgG - H&L (Alexa Fluor® 594), pre-adsorbed, used at 2μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
The secondary antibody only control displays negative staining, as expected.
Alexa Fluor® - Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (ab150072)
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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