AB181347

Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed

Name: Donkey F(ab')2 Anti Rabbit (IgG) secondary antibody preadsorbed Alexa Fluor® 647
Brand: Abcam Limited
SKU: AB181347
Price: 310 USD
Availability: InStock

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(11 Publications)

Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 11 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

2 Images

Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed (AB181347)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed (AB181347)

ICC/IF image of ab6046 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilzed with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 10% normal Donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 5μg/ml) overnight at +4°C. The secondary antibody (red) was ab181347 Alexa Fluor® 647 Donkey F(ab')2 polyclonal Secondary Antibody to Rabbit IgG (H+L) used at 1μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

Alexa Fluor® - Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed (AB181347)

Alexa Fluor®

Unknown

Alexa Fluor® - Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed (AB181347)

Key facts

Host species

Donkey

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Human

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Applications

ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

Reacts with the heavy and light chains of rabbit IgG and the light chains of rabbit IgM.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"" } } }

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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Gel filtration chromatography of pepsin digested antibody.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Rabbit IgG

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8724 PubMed41028013

2025

Munc13-1 restoration mitigates presynaptic pathology in spinal muscular atrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Mehri Moradi,Julia Weingart,Chunchu Deng,Mahoor Nasouti,Michael Briese,Sibylle Jablonka,Markus Sauer,Michael Sendtner

The Biochemical journal 479:1409-1428 PubMed35722941

2022

Binding partners regulate unfolding of myosin VI to activate the molecular motor.

Applications

Unspecified application

Species

Unspecified reactive species

Ália Dos Santos,Natalia Fili,Yukti Hari-Gupta,Rosemarie E Gough,Lin Wang,Marisa Martin-Fernandez,Jesse Aaron,Eric Wait,Teng-Leong Chew,Christopher P Toseland

Nature communications 13:1346 PubMed35292632

2022

Myosin VI regulates the spatial organisation of mammalian transcription initiation.

Applications

Unspecified application

Species

Unspecified reactive species

Yukti Hari-Gupta,Natalia Fili,Ália Dos Santos,Alexander W Cook,Rosemarie E Gough,Hannah C W Reed,Lin Wang,Jesse Aaron,Tomas Venit,Eric Wait,Andreas Grosse-Berkenbusch,J Christof M Gebhardt,Piergiorgio Percipalle,Teng-Leong Chew,Marisa Martin-Fernandez,Christopher P Toseland

Frontiers in immunology 13:751296 PubMed35296079

2022

Targeting Glycolysis in Alloreactive T Cells to Prevent Acute Graft--Host Disease While Preserving Graft-Versus-Leukemia Effect.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Huang,Yujing Zou,Yiqun Jiao,Peijie Shi,Xiaoli Nie,Wei Huang,Chuanfeng Xiong,Michael Choi,Charles Huang,Andrew N Macintyre,Amanda Nichols,Fang Li,Chuan-Yuan Li,Nancie J MacIver,Diana M Cardona,Todd V Brennan,Zhiguo Li,Nelson J Chao,Jeffrey C Rathmell,Benny J Chen

JCI insight 7: PubMed35041619

2022

Mutations in OSBPL2 cause hearing loss associated with primary cilia defects via sonic hedgehog signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Hairong Shi,Hongshun Wang,Cheng Zhang,Yajie Lu,Jun Yao,Zhibin Chen,Guangqian Xing,Qinjun Wei,Xin Cao

Nature protocols 17:890-909 PubMed35140411

2022

Spotting-based differentiation of functional dopaminergic progenitors from human pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jisun Kim,Jeha Jeon,Bin Song,Nayeon Lee,Sanghyeok Ko,Young Cha,Pierre Leblanc,Hyemyung Seo,Kwang-Soo Kim

Journal of cellular and molecular medicine 26:1229-1244 PubMed35023309

2022

Enhancing fractalkine/CX3CR1 signalling pathway can reduce neuroinflammation by attenuating microglia activation in experimental diabetic retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Mengmeng Jiang,Hai Xie,Chaoyang Zhang,Tianqin Wang,Haibin Tian,Lixia Lu,Jing-Ying Xu,Guo-Tong Xu,Lin Liu,Jingfa Zhang

The Journal of experimental medicine 216:556-570 PubMed30787033

2019

A major role for ferroptosis in -induced cell death and tissue necrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Eduardo P Amaral,Diego L Costa,Sivaranjani Namasivayam,Nicolas Riteau,Olena Kamenyeva,Lara Mittereder,Katrin D Mayer-Barber,Bruno B Andrade,Alan Sher

Scientific reports 7:16474 PubMed29184084

2017

The flexibility and dynamics of the tubules in the endoplasmic reticulum.

Applications

Unspecified application

Species

Unspecified reactive species

Pantelis Georgiades,Victoria J Allan,Graham D Wright,Philip G Woodman,Parinya Udommai,Manloeng A Chung,Thomas A Waigh

Frontiers in immunology 8:1575 PubMed29201030

2017

Toll-Like Receptor 4, but Not Neutrophil Extracellular Traps, Promote IFN Type I Expression to Enhance Th2 Responses to .

Applications

Unspecified application

Species

Unspecified reactive species

Christophe Pellefigues,Shiau-Choot Tang,Alfonso Schmidt,Ruby F White,Olivier Lamiable,Lisa M Connor,Christiane Ruedl,Jurek Dobrucki,Graham Le Gros,Franca Ronchese

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed

Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed (AB181347)

Alexa Fluor® - Donkey F(ab')2 Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed (AB181347)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (11)

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