AB7007

Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed

Name: Donkey F(ab')2 Anti Rabbit (IgG) secondary antibody preadsorbed PE (ab7007)
Brand: Abcam Limited
SKU: AB7007
Price: 520 USD
Availability: InStock

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(33 Publications)

Suitable for IM, ICC/IF, IHC-P, Flow Cyt, WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 33 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

3 Images

Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed (AB7007)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed (AB7007)

ab7007 was used at dilution 1/250 with the primary antibody ab24182 in ICC/IF. See the review on ab24182.

Flow Cytometry - Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed (AB7007)

Flow Cyt

Unknown

Flow Cytometry - Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed (AB7007)

ab7007 was used with the primary antibody ab18203 in Flow Cyt. See Abreview on ab18203.

Supplier Data

Western blot - Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed (AB7007)

Western blot analysis of ab7007 used as a secondary antibody at 1/1000 dilution for 60 min at RT.

All lanes:

Rabbit IgG at 50 ng

Secondary

All lanes:

Western blot - Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed (ab7007) at 1/1000 dilution

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Key facts

Host species

Donkey

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Sheep, Goat, Horse, Chicken, Guinea pig, Hamster, Cow, Human

Pre-adsorbed

Yes

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Applications

IM, ICC/IF, Flow Cyt, IHC-P, WB

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or to Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat & Sheep Serum Proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IM": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/100 - 1/250", "notes":"" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/200", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/100 - 1/250", "notes":"The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 ug of antibody conjugate." }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000", "notes":"" } } }

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Product details

Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

F(ab')2 Anti-Rabbit IgG (H&L) Phycoerythrin Conjugated Antibody Pre-adsorbed was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Rabbit IgG

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Publications (33)

Recent publications for all applications. Explore the full list and refine your search

Balkan medical journal 41:348-356 PubMed39129512

2024

Zn-Induced Cytotoxicity and Mitochondrial Stress in Microglia: Implications of the Protective Role of Immunoglobulin G In Vitro

Applications

Unspecified application

Species

Unspecified reactive species

Katarina Živančević,Başak Aru,Abdullah Demir,Lidija Radenović,Pavle Andjus,Gülderen Yanıkkaya Demirel

Cells 12: PubMed36611982

2023

Maternal Hyperhomocysteinemia Disturbs the Mechanisms of Embryonic Brain Development and Its Maturation in Early Postnatal Ontogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Dmitrii S Vasilev,Anastasiia D Shcherbitskaia,Natalia L Tumanova,Anastasiia V Mikhel,Yulia P Milyutina,Anna A Kovalenko,Nadezhda M Dubrovskaya,Daria B Inozemtseva,Irina V Zalozniaia,Alexander V Arutjunyan

Basic and clinical neuroscience 13:625-636 PubMed37313021

2022

Transdifferentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cells in Dopaminergic Neurons in a Three-Dimensional Culture.

Applications

Unspecified application

Species

Unspecified reactive species

Ardeshir Moayeri,Rafieh Alizadeh,Hatef Ghasemi Hamidabadi,Maryam Nazm Bojnordi,Ali Niapour,Azim Hedayatpour,Marzieh Darvishi,Fatemeh Heidari,Maryam Soleimani,Leila Elyasi

Frontiers in neuroscience 16:867120 PubMed35495064

2022

Maternal Hypoxia Increases the Excitability of Neurons in the Entorhinal Cortex and Dorsal Hippocampus of Rat Offspring.

Applications

Unspecified application

Species

Unspecified reactive species

Dmitry V Amakhin,Elena B Soboleva,Tatiana Yu Postnikova,Natalia L Tumanova,Nadezhda M Dubrovskaya,Daria S Kalinina,Dmitrii S Vasilev,Aleksey V Zaitsev

Oncogene 40:4884-4893 PubMed34163029

2021

SOX9 is a critical regulator of TSPAN8-mediated metastasis in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Junjian Li,Xiaoliang Chen,Liqun Zhu,Zhenghong Lao,Tianhao Zhou,Lijuan Zang,Weiyu Ge,Mengyi Jiang,Jingxuan Xu,Yuan Cao,Shaoqian Du,Yue Yu,Guangjian Fan,Hongxia Wang

Cells 10: PubMed34207057

2021

Prenatal Hyperhomocysteinemia Induces Glial Activation and Alters Neuroinflammatory Marker Expression in Infant Rat Hippocampus.

Applications

Unspecified application

Species

Unspecified reactive species

Anastasiia D Shcherbitskaia,Dmitrii S Vasilev,Yulia P Milyutina,Natalia L Tumanova,Anastasiia V Mikhel,Irina V Zalozniaia,Alexander V Arutjunyan

Molecular therapy oncolytics 20:187-198 PubMed33665357

2021

IRGM promotes melanoma cell survival through autophagy and is a promising prognostic biomarker for clinical application.

Applications

Unspecified application

Species

Unspecified reactive species

Linlu Tian,Hongxue Meng,Xiao Dong,Xinlei Li,Zilin Shi,Hulun Li,Lie Zhang,Yue Yang,Ruijie Liu,Chunying Pei,Bo Li,Hongwei Xu,Rui Li

Prostaglandins, leukotrienes, and essential fatty 163:102210 PubMed33242781

2020

Prostaglandin F2α in vitro can affect basic inflammatory parameters of mesenchymal stem cells and slight modulating some of their immunomodulatory properties.

Applications

Unspecified application

Species

Unspecified reactive species

Andressa Cristina Antunes Santos,Talita Sartori,Primavera Borelli,Ricardo Ambrosio Fock

Pharmaceuticals (Basel, Switzerland) 13: PubMed33113868

2020

Anakinra Reduces Epileptogenesis, Provides Neuroprotection, and Attenuates Behavioral Impairments in Rats in the Lithium-Pilocarpine Model of Epilepsy.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra V Dyomina,Olga E Zubareva,Ilya V Smolensky,Dmitry S Vasilev,Maria V Zakharova,Anna A Kovalenko,Alexander P Schwarz,Alexander M Ischenko,Aleksey V Zaitsev

PloS one 15:e0239292 PubMed33002044

2020

Silencing miR-202-3p increases MMP-1 and promotes a brain invasive phenotype in metastatic breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rania Harati,Shirin Hafezi,Aloïse Mabondzo,Abdelaziz Tlili

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed

Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed (AB7007)

Flow Cytometry - Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed (AB7007)

Western blot - Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed (AB7007)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (33)

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