Suitable for IM, ICC/IF, IHC-P, Flow Cyt, WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 33 publications.
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- Cells 12:2023Maternal Hyperhomocysteinemia Disturbs the Mechanisms of Embryonic Brain Development and Its Maturation in Early Postnatal Ontogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDmitrii S Vasilev,Anastasiia D Shcherbitskaia,Natalia L Tumanova,Anastasiia V Mikhel,Yulia P Milyutina,Anna A Kovalenko,Nadezhda M Dubrovskaya,Daria B Inozemtseva,Irina V Zalozniaia,Alexander V ArutjunyanPubMed 36611982
- Basic and clinical neuroscience 13:625-6362022Transdifferentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cells in Dopaminergic Neurons in a Three-Dimensional Culture.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArdeshir Moayeri,Rafieh Alizadeh,Hatef Ghasemi Hamidabadi,Maryam Nazm Bojnordi,Ali Niapour,Azim Hedayatpour,Marzieh Darvishi,Fatemeh Heidari,Maryam Soleimani,Leila ElyasiPubMed 37313021
- Frontiers in neuroscience 16:8671202022Maternal Hypoxia Increases the Excitability of Neurons in the Entorhinal Cortex and Dorsal Hippocampus of Rat Offspring.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDmitry V Amakhin,Elena B Soboleva,Tatiana Yu Postnikova,Natalia L Tumanova,Nadezhda M Dubrovskaya,Daria S Kalinina,Dmitrii S Vasilev,Aleksey V ZaitsevPubMed 35495064
- Oncogene 40:4884-48932021SOX9 is a critical regulator of TSPAN8-mediated metastasis in pancreatic cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunjian Li,Xiaoliang Chen,Liqun Zhu,Zhenghong Lao,Tianhao Zhou,Lijuan Zang,Weiyu Ge,Mengyi Jiang,Jingxuan Xu,Yuan Cao,Shaoqian Du,Yue Yu,Guangjian Fan,Hongxia WangPubMed 34163029
- Cells 10:2021Prenatal Hyperhomocysteinemia Induces Glial Activation and Alters Neuroinflammatory Marker Expression in Infant Rat Hippocampus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnastasiia D Shcherbitskaia,Dmitrii S Vasilev,Yulia P Milyutina,Natalia L Tumanova,Anastasiia V Mikhel,Irina V Zalozniaia,Alexander V ArutjunyanPubMed 34207057
- Molecular therapy oncolytics 20:187-1982021IRGM promotes melanoma cell survival through autophagy and is a promising prognostic biomarker for clinical application.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLinlu Tian,Hongxue Meng,Xiao Dong,Xinlei Li,Zilin Shi,Hulun Li,Lie Zhang,Yue Yang,Ruijie Liu,Chunying Pei,Bo Li,Hongwei Xu,Rui LiPubMed 33665357
- Prostaglandins, leukotrienes, and essential fatty 163:1022102020Prostaglandin F2α in vitro can affect basic inflammatory parameters of mesenchymal stem cells and slight modulating some of their immunomodulatory properties.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndressa Cristina Antunes Santos et. al.PubMed 33242781
- Pharmaceuticals (Basel, Switzerland) 13:2020Anakinra Reduces Epileptogenesis, Provides Neuroprotection, and Attenuates Behavioral Impairments in Rats in the Lithium-Pilocarpine Model of Epilepsy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexandra V Dyomina et. al.PubMed 33113868
- PloS one 15:e02392922020Silencing miR-202-3p increases MMP-1 and promotes a brain invasive phenotype in metastatic breast cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRania Harati et. al.PubMed 33002044
Key facts
- Host species
- Donkey
- Target species
- Rabbit
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Rat, Sheep, Goat, Horse, Chicken, Guinea pig, Hamster, Cow, Human
- Conjugation
- PE
- Excitation/Emission
- Ex: 480;565nm, Em: 578nm
- Applications
- IM, ICC/IF, IHC-P, Flow Cyt, WB
- Clonality
- Polyclonal
- Format
- F(ab')2
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application IM | Reactivity Expected | Dilution info - | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/100 - 1/250 | Notes - |
Application IHC-P | Reactivity Expected | Dilution info 1/200 | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/100 - 1/250 | Notes The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 ug of antibody conjugate. |
Application WB | Reactivity Reacts | Dilution info 1/1000 | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS
Recommended products
Suitable for IM, ICC/IF, IHC-P, Flow Cyt, WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 33 publications.
Key facts
- Description
- Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed
- Host species
- Donkey
- Target species
- Rabbit
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Rat, Sheep, Goat, Horse, Chicken, Guinea pig, Hamster, Cow, Human
- Conjugation
- PE
- Excitation/Emission
- Ex: 480;565nm, Em: 578nm
- Applications
- IM, ICC/IF, IHC-P, Flow Cyt, WB
- Clonality
- Polyclonal
- Format
- F(ab')2
- Pre-adsorbed
- Yes
- Specificity
No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or to Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat & Sheep Serum Proteins.
- Isotype
- IgG
- Concentration
Properties
- Form
- Lyophilized
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Purification technique
- Affinity purification
- Purification notes
F(ab')2 Anti-Rabbit IgG (H&L) Phycoerythrin Conjugated Antibody Pre-adsorbed was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Product promise
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Full details and terms and conditions can be found here:
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2 product images
Western blot - Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed (ab7007)
Western blot analysis of ab7007 used as a secondary antibody at 1/1000 dilution for 60 min at RT.
All lanes: Rabbit IgG at 50 ng
SecondaryAll lanes: Western blot - Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed (ab7007) at 1/1000 dilution
Flow Cytometry - Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed (ab7007)
ab7007 was used with the primary antibody Anti-N Cadherin antibody - Intercellular Junction Marker ab18203 in Flow Cyt. See Abreview on Anti-N Cadherin antibody - Intercellular Junction Marker ab18203.
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