Donkey F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed (AB150149)

HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (green) was ab150149 Alexa Fluor® 488 donkey F(ab)2 anti-rat IgG (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed (AB150149)

ICC/IF image of ab6160 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6160, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab150149 Alexa Fluor® 488 donkey F(ab)2 anti-rat IgG (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• Alexa Fluor®

Unknown

Alexa Fluor® - Donkey F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed (AB150149)