AB150150

Donkey F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed

Name: Donkey F(ab')2 Anti-Rat IgG (Alexa Fluor® 555) preadsorbed secondary antibody (ab150150) | Abcam
Brand: Abcam Limited
SKU: AB150150

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(3 Publications)

Suitable for IHC-Fr, ICC/IF, IHC-P, Flow Cyt, ELISA. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 3 publications.

View Alternative Names

Ig gamma-1 chain C region

2 Images

Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed (AB150150)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed (AB150150)

ICC/IF image of ab6160 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6160, 1μg/ml) overnight at +4°C. The secondary antibody (yellow) was ab150150, Donkey F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed, used at 1μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

The secondary antibody only control displays negative staining, as expected.

Alexa Fluor® - Donkey F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed (AB150150)

Alexa Fluor®

Unknown

Alexa Fluor® - Donkey F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed (AB150150)

Key facts

Host species

Donkey

Target species

Rat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse,

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Applications

ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF

applications

Clonality

Polyclonal

Isotype

IgG

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Reacts with the heavy and light chains of rat IgG as demonstrated by ELISA. May also react with other species and with the light chains of other rat immunoglobulins. Minimal cross reaction to mouse serum proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"Use at an assay dependent dilution" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"Use at an assay dependent dilution" } } }

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Product details

This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules were removed.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ig gamma-1 chain C region

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed36902241

2023

Analysis of the Role of Stellate Cell VCAM-1 in NASH Models in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kyoung-Jin Chung,Aigli-Ioanna Legaki,Grigorios Papadopoulos,Bettina Gercken,Janine Gebler,Robert F Schwabe,Triantafyllos Chavakis,Antonios Chatzigeorgiou

iScience 25:103693 PubMed35036877

2022

Deciphering cellular signals in adult mouse sinoatrial node cells.

Applications

Unspecified application

Species

Unspecified reactive species

Gopireddy R Reddy,Lu Ren,Phung N Thai,Jessica L Caldwell,Manuela Zaccolo,Julie Bossuyt,Crystal M Ripplinger,Yang K Xiang,Madeline Nieves-Cintrón,Nipavan Chiamvimonvat,Manuel F Navedo

PloS one 11:e0153522 PubMed27124483

2016

Real-Time Tracking of BODIPY-C12 Long-Chain Fatty Acid in Human Term Placenta Reveals Unique Lipid Dynamics in Cytotrophoblast Cells.

Applications

Species

Unspecified reactive species

Kevin Kolahi,Samantha Louey,Oleg Varlamov,Kent Thornburg

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed

Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed (AB150150)

Alexa Fluor® - Donkey F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed (AB150150)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Immunogen

Specificity

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

Product promise