Suitable for IHC-Fr, ICC/IF, IHC-P, Flow Cyt, ELISA. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 3 publications.
Images
Publications
Filter by
- International journal of molecular sciences 24:2023Analysis of the Role of Stellate Cell VCAM-1 in NASH Models in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKyoung-Jin Chung,Aigli-Ioanna Legaki,Grigorios Papadopoulos,Bettina Gercken,Janine Gebler,Robert F Schwabe,Triantafyllos Chavakis,Antonios ChatzigeorgiouPubMed 36902241
- iScience 25:1036932022Deciphering cellular signals in adult mouse sinoatrial node cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGopireddy R Reddy,Lu Ren,Phung N Thai,Jessica L Caldwell,Manuela Zaccolo,Julie Bossuyt,Crystal M Ripplinger,Yang K Xiang,Madeline Nieves-Cintrón,Nipavan Chiamvimonvat,Manuel F NavedoPubMed 35036877
- PloS one 11:e01535222016Real-Time Tracking of BODIPY-C12 Long-Chain Fatty Acid in Human Term Placenta Reveals Unique Lipid Dynamics in Cytotrophoblast Cells.
Key facts
- Host species
- Donkey
- Target species
- Rat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse
- Conjugation
- Alexa Fluor® 555
- Excitation/Emission
- Ex: 555nm, Em: 565nm
- Applications
- IHC-Fr, ICC/IF, IHC-P, Flow Cyt, ELISA
- Clonality
- Polyclonal
- Format
- F(ab')2
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes Use at an assay dependent dilution |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000 | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes Use at an assay dependent dilution |
Alternative names
Ig gamma-1 chain C region
Recommended products
Suitable for IHC-Fr, ICC/IF, IHC-P, Flow Cyt, ELISA. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 3 publications.
Key facts
- Description
- Donkey F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed
- Host species
- Donkey
- Target species
- Rat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse
- Conjugation
- Alexa Fluor® 555
- Excitation/Emission
- Ex: 555nm, Em: 565nm
- Applications
- IHC-Fr, ICC/IF, IHC-P, Flow Cyt, ELISA
- Clonality
- Polyclonal
- Format
- F(ab')2
- Pre-adsorbed
- Yes
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Specificity
Reacts with the heavy and light chains of rat IgG as demonstrated by ELISA. May also react with other species and with the light chains of other rat immunoglobulins. Minimal cross reaction to mouse serum proteins.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules were removed.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed (ab150150)
ICC/IF image of Anti-Tubulin antibody [YL1/2] - Loading Control ab6160 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-Tubulin antibody [YL1/2] - Loading Control ab6160, 1μg/ml) overnight at +4°C. The secondary antibody (yellow) was ab150150, Donkey F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed, used at 1μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
The secondary antibody only control displays negative staining, as expected.
Alexa Fluor® - Donkey F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed (ab150150)
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com