Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 2 publications.
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- Journal of developmental biology 11:2023Decreased Expression of Pulmonary Homeobox NKX2.1 and Surfactant Protein C in Developing Lungs That Over-Express Receptors for Advanced Glycation End-Products (RAGE).Applications:Unspecified applicationReactive species:Unspecified reactive speciesDerek M Clarke,Katrina L Curtis,Ryan A Wendt,Brendan M Stapley,Evan T Clark,Nathan Beckett,Kennedy M Campbell,Juan A Arroyo,Paul R ReynoldsPubMed 37489334
- Journal of developmental biology 10:2022Involvement of a Basic Helix-Loop-Helix Gene in Specification of Chicken Retinal Pigment Epithelium.Applications:Unspecified applicationReactive species:Unspecified reactive speciesToshiki Kinuhata,Keita Sato,Tetsuya Bando,Taro Mito,Satoru Miyaishi,Tsutomu Nohno,Hideyo OhuchiPubMed 36412639
Key facts
- Host species
- Donkey
- Target species
- Rat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alexa Fluor® 568
- Excitation/Emission
- Ex: 578nm, Em: 603nm
- Applications
- ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt
- Clonality
- Polyclonal
- Format
- F(ab')2
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000 | Notes - |
Alternative names
Ig gamma-1 chain C region
Recommended products
Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 2 publications.
Key facts
- Description
- Donkey F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 568) preadsorbed
- Host species
- Donkey
- Target species
- Rat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alexa Fluor® 568
- Excitation/Emission
- Ex: 578nm, Em: 603nm
- Applications
- ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt
- Clonality
- Polyclonal
- Format
- F(ab')2
- Pre-adsorbed
- Yes
- Specificity
Reacts with the heavy and light chains of rat IgG as demonstrated by ELISA. May also react with other species and with the light chains of other rat immunoglobulins. Minimal cross reaction to mouse serum proteins.
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
- Affinity purification Immunogen
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Product promise
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3 product images
Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 568) preadsorbed (ab175708)
ICC/IF image of Anti-Tubulin antibody [YL1/2] - Loading Control ab6160 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-Tubulin antibody [YL1/2] - Loading Control ab6160, 1μg/ml) overnight at +4°C. The secondary antibody (yellow) was ab175708 Alexa Fluor® 568 donkey F(ab)2 anti-rat IgG (H+L) used at 1μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
Alexa Fluor® 568 is pseudocolored in yellow.
Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 568) preadsorbed (ab175708)
HeLa cells showing negative staining by ICC/IF using secondary antibody only. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (yellow) was ab175708 Alexa Fluor® 568 donkey F(ab)2 anti-rat IgG (H+L) used at 1μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
Alexa Fluor® - Donkey F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 568) preadsorbed (ab175708)
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