Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 6 publications.
Images
Publications
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- Disease models & mechanisms 15:2022Therapeutic potential of macrophage colony-stimulating factor in chronic liver disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSahar Keshvari,Berit Genz,Ngari Teakle,Melanie Caruso,Michelle F Cestari,Omkar L Patkar,Brian W C Tse,Kamil A Sokolowski,Hilmar Ebersbach,Julia Jascur,Kelli P A MacDonald,Gregory Miller,Grant A Ramm,Allison R Pettit,Andrew D Clouston,Elizabeth E Powell,David A Hume,Katharine M IrvinePubMed 35169835
- Cell reports methods 1:1000682021Interrogating RNA and protein spatial subcellular distribution in smFISH data with DypFISH.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnca F Savulescu,Robyn Brackin,Emmanuel Bouilhol,Benjamin Dartigues,Jonathan H Warrell,Mafalda R Pimentel,Nicolas Beaume,Isabela C Fortunato,Stephane Dallongeville,Mikaël Boulle,Hayssam Soueidan,Fabrice Agou,Jan Schmoranzer,Jean-Christophe Olivo-Marin,Claudio A Franco,Edgar R Gomes,Macha Nikolski,Musa M MhlangaPubMed 35474672
- Bio-protocol 10:e37832020Multitarget Immunohistochemistry for Confocal and Super-resolution Imaging of Plant Cell Wall Polysaccharides.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKalina T Haas,Methieu Rivière,Raymond Wightman,Alexis PeaucellePubMed 33659438
- Current protocols in immunology 130:e1002020Characterization of Anaphylatoxin Receptor Expression and C3a/C5a Functions in Anaphylatoxin Receptor Reporter Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYves Laumonnier,Christian M Karsten,Gabriele Köhl,Jörg KöhlPubMed 32710701
- Scientific reports 10:107962020Germ cell depletion in recipient testis has adverse effects on spermatogenesis in orthotopically transplanted testis pieces via retinoic acid insufficiency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAkihiro Tsuchimoto,Masaaki Tone,Narumi Ogonuki,Masashi Hada,Atsuo Ogura,Seiji TakashimaPubMed 32612133
- Medical science monitor : international medical jo 22:3595-36042016Glaucocalyxin A Ameliorates Myocardial Ischemia-Reperfusion Injury in Mice by Suppression of Microvascular Thrombosis.
Key facts
- Host species
- Donkey
- Target species
- Rat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse
- Conjugation
- Alexa Fluor® 647
- Excitation/Emission
- Ex: 650nm, Em: 665nm
- Applications
- ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF
- Clonality
- Polyclonal
- Format
- F(ab')2
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000 | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Alternative names
Ig gamma-1 chain C region
Recommended products
Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 6 publications.
Key facts
- Description
- Donkey F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed
- Host species
- Donkey
- Target species
- Rat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse
- Conjugation
- Alexa Fluor® 647
- Excitation/Emission
- Ex: 650nm, Em: 665nm
- Applications
- ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF
- Clonality
- Polyclonal
- Format
- F(ab')2
- Pre-adsorbed
- Yes
- Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Product promise
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3 product images
Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150151)
ICC/IF image of Anti-Tubulin antibody [YL1/2] - Loading Control ab6160 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-Tubulin antibody [YL1/2] - Loading Control ab6160, 1μg/ml) overnight at +4°C. The secondary antibody (red) was ab150151 Alexa Fluor® 647 donkey F(ab)2 anti-rat IgG (H+L) used at 1μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150151)
HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (red) was ab150151 Alexa Fluor® 647 donkey F(ab)2 anti-rat IgG (H+L) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Alexa Fluor® - Donkey F(ab')2 Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150151)
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