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AB7009

Donkey F(ab')2 Anti-Sheep IgG H&L (PE) preadsorbed

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(5 Publications)

Suitable for ICC/IF, IHC-P, IHC-Fr, IM, Flow Cyt. Preadsorbed to minimise non-specific binding and high background staining. Cited in 5 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

Key facts

Host species

Donkey

Target species

Sheep

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Hamster, Human

Pre-adsorbed

Yes

Conjugation

PE

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Applications

Flow Cyt, IM, IHC-P, ICC/IF, IHC-Fr

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

No reaction was observed against anti-Pepsin, anti-Donkey IgG Fc, or Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit or Rat Serum Proteins.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC/IF": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/100 - 1/250", "notes":"<p></p>" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/50 - 1/200", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/50 - 1/200", "notes":"<p></p>" }, "IM": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/50 - 1/200", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/100 - 1/250", "notes":"<p></p>" } } }

Product details

Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Storage buffer
Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Product protocols

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Acta neuropathologica communications 13:118 PubMed40420167

2025

Therapeutic potential of NGF-enriched extracellular vesicles in modulating neuroinflammation and enhancing peripheral nerve remyelination.

Applications

Unspecified application

Species

Unspecified reactive species

Hancheol Yeo,Yoo Jung Kim,Jaekwon Seok,Yeonjoo Kwak,Soo Bin Jang,Na Hee Lim,Kwonwoo Song,Junghoon Lee,Min Chul Cho,Soo Woong Kim,Ssang-Goo Cho

International journal of stem cells 17:407-417 PubMed39396918

2024

Efficient Treatment of Psoriasis Using Conditioned Media from Mesenchymal Stem Cell Spheroids Cultured to Produce Transforming Growth Factor-1-Enriched Small-Sized Extracellular Vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Myeongjin Song,Kyung Min Lim,Kwonwoo Song,Geun-Ho Kang,Se Jong Kim,Youngseo Lee,Sujin Yu,Ki-Heon Jeong,Ssang-Goo Cho

International journal of molecular sciences 24: PubMed38003648

2023

Thermostable Basic Fibroblast Growth Factor Enhances the Production and Activity of Human Wharton's Jelly Mesenchymal Stem Cell-Derived Extracellular Vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

SangRok Park,SeJong Kim,KyungMin Lim,YeoKyung Shin,Kwonwoo Song,Geun-Ho Kang,Dae Young Kim,Hang-Cheol Shin,Ssang-Goo Cho

International journal of molecular sciences 24: PubMed37108128

2023

Improved Wound Healing and Skin Regeneration Ability of 3,2'-Dihydroxyflavone-Treated Mesenchymal Stem Cell-Derived Extracellular Vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Sehee Kim,Yeokyung Shin,Yujin Choi,Kyung-Min Lim,Yeojin Jeong,Ahmed Abdal Dayem,Yoonjoo Lee,Jongyub An,Kwonwoo Song,Soo Bin Jang,Ssang-Goo Cho

Stem cells and development 17:367-81 PubMed18447651

2008

Sustained macroscopic engraftment of cynomolgus embryonic stem cells in xenogeneic large animals after in utero transplantation.

Applications

Flow Cyt

Species

Unspecified reactive species

Yujiro Tanaka,Shinichiro Nakamura,Hiroaki Shibata,Yukiko Kishi,Tamako Ikeda,Shigeo Masuda,Kyoko Sasaki,Tomoyuki Abe,Satoshi Hayashi,Yoshihiro Kitano,Yoshikazu Nagao,Yutaka Hanazono
View all publications

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