Skip to main content

Suitable for ICC/IF, IHC-P, IHC-Fr, IM, Flow Cyt. Preadsorbed to minimise non-specific binding and high background staining. Cited in 2 publications.

Be the first to review this product! Submit a review

Publications

Key facts

Host species
Donkey
Target species
Sheep
Target isotype
IgG
Target specificity
Heavy & Light chains
Minimal cross-reactivity
Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Hamster, Human
Conjugation
PE
Excitation/Emission
Ex: 480;565nm, Em: 578nm
Applications
ICC/IF, IHC-P, IHC-Fr, IM, Flow Cyt
Clonality
Polyclonal
Format
F(ab')2

Reactivity data

Application
ICC/IF
Reactivity
Expected
Dilution info
1/100 - 1/250
Notes

-

Application
IHC-P
Reactivity
Expected
Dilution info
1/50 - 1/200
Notes

-

Application
IHC-Fr
Reactivity
Expected
Dilution info
1/50 - 1/200
Notes

-

Application
IM
Reactivity
Expected
Dilution info
1/50 - 1/200
Notes

-

Application
Flow Cyt
Reactivity
Expected
Dilution info
1/100 - 1/250
Notes

-

Target data

Alternative names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

Recommended products

Suitable for ICC/IF, IHC-P, IHC-Fr, IM, Flow Cyt. Preadsorbed to minimise non-specific binding and high background staining. Cited in 2 publications.

Key facts

Description
Donkey F(ab')2 Anti-Sheep IgG H&L (PE) preadsorbed
Host species
Donkey
Target species
Sheep
Target isotype
IgG
Target specificity
Heavy & Light chains
Minimal cross-reactivity
Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Hamster, Human
Conjugation
PE
Excitation/Emission
Ex: 480;565nm, Em: 578nm
Applications
ICC/IF, IHC-P, IHC-Fr, IM, Flow Cyt
Clonality
Polyclonal
Format
F(ab')2
Pre-adsorbed
Yes
Specificity

No reaction was observed against anti-Pepsin, anti-Donkey IgG Fc, or Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit or Rat Serum Proteins.

Isotype
IgG
Concentration
Loading...

Properties

Form
Liquid
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Purification technique
Affinity purification
Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

0 product images

    Downloads

    Fluorochrome chart

    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

    For licensing inquiries, please contact partnerships@abcam.com