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AB175664

Donkey Anti-Goat IgG H&L (Alexa Fluor® 405)

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(43 Publications)

Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Cited in 43 publications.
2 Images
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 405) (AB175664)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 405) (AB175664)

ICC/IF image of ab7291 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1μg/ml) overnight at +4°C. ab98800 (goat anti-mouse IgG) was then added as a secondary bridging antibody, at 1/250 dilution for 1h. ab175664 Alexa Fluor® 405 donkey anti-goat IgG (H+L) (blue) was then used at 2μg/ml for 1h as a tertiary antibody. DRAQ5 (ab108410) was used to stain the cell nuclei (red) at a concentration of 1.25μM.

The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 405) (AB175664)
  • Alexa Fluor®

Unknown

Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 405) (AB175664)

Key facts

Host species

Donkey

Target species

Goat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Alexa Fluor® 405

Excitation/Emission

Ex: 401nm, Em: 421nm

Applications

IHC-Fr, ICC/IF, ELISA, Flow Cyt, IHC-P

applications

Clonality

Polyclonal

Isotype

IgG

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"<p>We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor<sup>®</sup> 405 dye (see difference below)<br>Ex max: Alexa Fluor<sup>®</sup> 405 = 402nm / DAPI = 359nm<br>Em max: Alexa Fluor<sup>®</sup> 405 = 421nm / DAPI = 461nm</p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<p></p>" } } }

Product details

We recommend storage time at 4°C should be minimal, since this may affect the signal strength.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

Product protocols

Publications (43)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:6992 PubMed40738880

2025

Dual lineage origins contribute to neocortical astrocyte diversity.

Applications

Unspecified application

Species

Unspecified reactive species

Jiafeng Zhou,Ilaria Vitali,Sergi Roig-Puiggros,Awais Javed,Iva Cantando,Matteo Puglisi,Paola Bezzi,Denis Jabaudon,Christian Mayer,Riccardo Bocchi

Nature neuroscience 28:1622-1634 PubMed40670683

2025

Evidence for trans-synaptic propagation of oligomeric tau in human progressive supranuclear palsy.

Applications

Unspecified application

Species

Unspecified reactive species

Robert I McGeachan,Lois Keavey,Elizabeth M Simzer,Ya Yin Chang,Jamie L Rose,Maxwell P Spires-Jones,Mollie Gilmore,Kristjan Holt,Soraya Meftah,Natalia Ravingerova,Cristina Scutariu,Lewis W Taylor,Declan King,Makis Tzioras,Jane Tulloch,Sam A Booker,Imran Liaquat,Nicole Hindley-Pollock,Bethany Geary,Colin Smith,Paul M Brennan,Claire S Durrant,Tara L Spires-Jones

Nature biomedical engineering 9:1531-1546 PubMed40360762

2025

Focused ultrasound-microbubble treatment arrests the growth and formation of cerebral cavernous malformations.

Applications

Unspecified application

Species

Unspecified reactive species

Delaney G Fisher,Tanya Cruz,Matthew R Hoch,Khadijeh A Sharifi,Ishaan M Shah,Catherine M Gorick,Victoria R Breza,Anna C Debski,Joshua D Samuels,Jason P Sheehan,David Schlesinger,David Moore,James W Mandell,John R Lukens,G Wilson Miller,Petr Tvrdik,Richard J Price

Cell death discovery 11:90 PubMed40050290

2025

CXCL16 knockout inhibit asthma airway inflammation by suppressing H2-DM molecular mediated antigen presentation.

Applications

Unspecified application

Species

Unspecified reactive species

Ting-Ting Liu,Zhi Zhang,Jing Deng,Chang-Yu Shi,Shuai Zheng,Li-Xin Jia,Jie Du,Chunmei Piao

iScience 27:111276 PubMed39628560

2024

The first interneuron of the mouse visual system is tailored to the natural environment through morphology and electrical coupling.

Applications

Unspecified application

Species

Unspecified reactive species

Matteo Spinelli,Alejandra Acevedo Harnecker,Christoph T Block,Lucia Lindenthal,Fabian Schuhmann,Martin Greschner,Ulrike Janssen-Bienhold,Karin Dedek,Christian Puller

iScience 27:110031 PubMed38868192

2024

Spatiotemporal sphingosine-1-phosphate receptor 3 expression within the cerebral vasculature after ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Hana Matuskova,Lisa T Porschen,Frank Matthes,Arne G Lindgren,Gabor C Petzold,Anja Meissner

Nature communications 15:4822 PubMed38844769

2024

Months-long tracking of neuronal ensembles spanning multiple brain areas with Ultra-Flexible Tentacle Electrodes.

Applications

Unspecified application

Species

Unspecified reactive species

Tansel Baran Yasar,Peter Gombkoto,Alexei L Vyssotski,Angeliki D Vavladeli,Christopher M Lewis,Bifeng Wu,Linus Meienberg,Valter Lundegardh,Fritjof Helmchen,Wolfger von der Behrens,Mehmet Fatih Yanik

BMC nephrology 24:378 PubMed38114895

2023

In vivo characterization of a podocyte-expressed short podocin isoform.

Applications

Unspecified application

Species

Unspecified reactive species

Linus Butt,David Unnersjö-Jess,Dervla Reilly,Robert Hahnfeldt,Markus M Rinschen,Katarzyna Bozek,Bernhard Schermer,Thomas Benzing,Martin Höhne

International journal of molecular sciences 24: PubMed37834299

2023

Aβ1-42 Accumulation Accompanies Changed Expression of Ly6/uPAR Proteins, Dysregulation of the Cholinergic System, and Degeneration of Astrocytes in the Cerebellum of Mouse Model of Early Alzheimer Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Maxim L Bychkov,Aizek B Isaev,Alexander A Andreev-Andrievskiy,Konstantin Petrov,Alexander S Paramonov,Mikhail P Kirpichnikov,Ekaterina N Lyukmanova

Aging and disease 14:1870-1886 PubMed37196130

2023

Optogenetic Activation of Astrocytes Reduces Blood-Brain Barrier Disruption via IL-10 In Stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Suo,Lidong Deng,Tingting Chen,Shengju Wu,Lin Qi,Ze Liu,Tingting He,Heng-Li Tian,Wanlu Li,Yaohui Tang,Guo-Yuan Yang,Zhijun Zhang
View all publications

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