Donkey Anti-Goat IgG H&L (Alexa Fluor® 405)
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(43 Publications)
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 405) (AB175664)
ICC/IF image of ab7291 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1μg/ml) overnight at +4°C. ab98800 (goat anti-mouse IgG) was then added as a secondary bridging antibody, at 1/250 dilution for 1h. ab175664 Alexa Fluor® 405 donkey anti-goat IgG (H+L) (blue) was then used at 2μg/ml for 1h as a tertiary antibody. DRAQ5 (ab108410) was used to stain the cell nuclei (red) at a concentration of 1.25μM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
- Alexa Fluor®
Unknown
Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 405) (AB175664)
Reactivity data
Product details
We recommend storage time at 4°C should be minimal, since this may affect the signal strength.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Publications (43)
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Nature communications 16:6992 PubMed40738880
2025
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Nature neuroscience 28:1622-1634 PubMed40670683
2025
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Nature biomedical engineering 9:1531-1546 PubMed40360762
2025
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Cell death discovery 11:90 PubMed40050290
2025
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iScience 27:111276 PubMed39628560
2024
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iScience 27:110031 PubMed38868192
2024
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Nature communications 15:4822 PubMed38844769
2024
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BMC nephrology 24:378 PubMed38114895
2023
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International journal of molecular sciences 24: PubMed37834299
2023
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Aging and disease 14:1870-1886 PubMed37196130
2023
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com