Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 15 publications.
Images
Publications
Filter by
- PLoS pathogens 20:e10117102024Deficiency in astrocyte CCL2 production reduces neuroimmune control of Toxoplasma gondii infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStephanie B Orchanian,Katherine Still,Tajie H Harris,Melissa B LodoenPubMed 38206985
- Cell reports 42:1124302023A synaptic molecular dependency network in knockdown of autism- and schizophrenia-associated genes revealed by multiplexed imaging.Applications:Unspecified applicationReactive species:Unspecified reactive speciesReuven Falkovich,Eric W Danielson,Karen Perez de Arce,Eike-C Wamhoff,Juliana Strother,Anna P Lapteva,Morgan Sheng,Jeffrey R Cottrell,Mark BathePubMed 37099425
- Nutrients 15:2023Stimulation of GHRH Neuron Axon Growth by Leptin and Impact of Nutrition during Suckling in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLyvianne Decourtye-Espiard,Maud Clemessy,Patricia Leneuve,Erik Mire,Tatiana Ledent,Yves Le Bouc,Laurent KappelerPubMed 36904077
- eLife 11:2022Spinal premotor interneurons controlling antagonistic muscles are spatially intermingled.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRemi Ronzano,Sophie Skarlatou,Bianca K Barriga,B Anne Bannatyne,Gardave Singh Bhumbra,Joshua D Foster,Jeffrey D Moore,Camille Lancelin,Amanda M Pocratsky,Mustafa Görkem Özyurt,Calvin Chad Smith,Andrew J Todd,David J Maxwell,Andrew J Murray,Samuel L Pfaff,Robert M Brownstone,Niccolò Zampieri,Marco BeatoPubMed 36512397
- Nature communications 13:50792022A brainstem monosynaptic excitatory pathway that drives locomotor activities and sympathetic cardiovascular responses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSatoshi Koba,Nao Kumada,Emi Narai,Naoya Kataoka,Kazuhiro Nakamura,Tatsuo WatanabePubMed 36038592
- Autophagy 18:2711-27302022RTN4/Nogo-A-S1PR2 negatively regulates angiogenesis and secondary neural repair through enhancing vascular autophagy in the thalamus after cerebral cortical infarction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeiyi Xiao,Jinmin Gu,Wei Xu,Xingyang Niu,Jian Zhang,Jingjing Li,Yicong Chen,Zhong Pei,Jinsheng Zeng,Shihui XingPubMed 35263212
- eLife 10:2021Proximal and distal spinal neurons innervating multiple synergist and antagonist motor pools.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRemi Ronzano,Camille Lancelin,Gardave Singh Bhumbra,Robert M Brownstone,Marco BeatoPubMed 34727018
- Autophagy 18:990-10042021USP5 attenuates NLRP3 inflammasome activation by promoting autophagic degradation of NLRP3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBaoshan Cai,Jian Zhao,Yuling Zhang,Yaxing Liu,Chunhong Ma,Fan Yi,Yi Zheng,Lei Zhang,Tian Chen,Huiqing Liu,Bingyu Liu,Chengjiang GaoPubMed 34486483
- Frontiers in neuroscience 15:6469142021Inflammatory Foreign Body Response Induced by Neuro-Implants in Rat Cortices Depleted of Resident Microglia by a CSF1R Inhibitor and Its Implications.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAviv Sharon,Maciej M Jankowski,Nava Shmoel,Hadas Erez,Micha E SpiraPubMed 33841088
- Frontiers in neuroscience 14:9262020Immunohistological and Ultrastructural Study of the Inflammatory Response to Perforated Polyimide Cortical Implants: Mechanisms Underlying Deterioration of Electrophysiological Recording Quality.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShun-Ho Huang et. al.PubMed 32982683
Key facts
- Host species
- Donkey
- Target species
- Goat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Rat, Rabbit, Chicken, Human, Pig
- Conjugation
- Alexa Fluor® 405
- Excitation/Emission
- Ex: 401nm, Em: 421nm
- Applications
- ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200.00000 - 1/1000.00000 | Notes We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor® 405 dye (see difference below) |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000 | Notes - |
Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G
Recommended products
Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 15 publications.
Key facts
- Description
- Donkey Anti-Goat IgG H&L (Alexa Fluor® 405) preadsorbed
- Host species
- Donkey
- Target species
- Goat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Rat, Rabbit, Chicken, Human, Pig
- Conjugation
- Alexa Fluor® 405
- Excitation/Emission
- Ex: 401nm, Em: 421nm
- Applications
- ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt
- Clonality
- Polyclonal
- Pre-adsorbed
- Yes
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with goat IgG and with light chains common to other goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to chicken, human, mouse, pig, rabbit and rat IgG was detected. This antibody may cross react with IgG from other species.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
Antiserum was cross adsorbed using chicken, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive Antibodies. The antibody to goat IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
We recommend storage time at 4°C should be minimal, since this may affect the signal strength.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 405) preadsorbed (ab175665)
ICC/IF image of Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the primary antibody (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, 1μg/ml) overnight at +4°C. ab98800, goat anti-mouse IgG, was then added as a secondary bridging antibody, at 1/250 dilution for 1h. ab175665 Alexa Fluor® 405 Donkey polyclonal Secondary Antibody to Goat IgG - H&L pre-adsorbed (blue) was then used at 1μg/ml for 1h as a tertiary antibody. DRAQ5™ (DRAQ5™ ab108410) was used to stain the cell nuclei (red) at a concentration of 1.25μM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
This image is courtesy of a customer review submitted by Bryan NiedenbergerImmunohistochemistry (Frozen sections) - Donkey Anti-Goat IgG H&L (Alexa Fluor® 405) preadsorbed (ab175665)
Postnatal day 6 mouse testes were fixed with 4% paraformaldehyde. Tissue was embedded in O.C.T. and frozen. 5 micron sections were cut and transferred to slides. Sections were permeabilized with 0.1% Triton X-100 in PBS, and blocked with 3% BSA in 0.1% Triton X-100 + PBS. Sections were incubated with either (A) no primary antibody or (B) Goat Anti-DDX4 at a 1/800 dilution for 1 h at RT. Sections were then washed 3X with 0.1% Triton X-100 in PBS and Donkey Anti-Goat IgG H&L (Alexa Fluor® 405) (ab175665) applied at a 1/500 dilution. Sections were then mounted after washing 3X with 0.1% Triton X-100 in PBS.
Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 405) preadsorbed (ab175665)
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com