Donkey anti-goat IgG H&L (Alexa Fluor® 488) is a secondary antibody with a maximum absorption wavelength of 495nm and a maximum emission wavelength of 519nm. Ideal for fluorescent cell and tissue imaging. Suitable for ICC/IF, IHC, flow cytometry and ELISA.
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 360 publications
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- Immunity, inflammation and disease 12:e10982024The Lnc-ENST00000602558/IGF1 axis as a predictor of response to treatment with tripterygium glycosides in rheumatoid arthritis patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Gao,Xiaoyue Wang,Yanfeng Gao,Jian Bai,Yanpeng Zhao,Renyi Wang,Hanzhou Wang,Guangzhao Zhu,Xixi Wang,Xiaochen Han,Yanqiong Zhang,Hailong WangPubMed 38270302
- Cell death & disease 15:362024Cortical microinfarcts potentiate recurrent ischemic injury through NLRP3-dependent trained immunity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYiwei Feng,Lishan Lin,Tengteng Wu,Yukun Feng,Fengyin Liang,Ge Li,Yongchao Li,Yalun Guan,Shuhua Liu,Yu Zhang,Guangqing Xu,Zhong PeiPubMed 38216560
- Communications biology 7:32024Activation of M1 cholinergic receptors in mouse somatosensory cortex enhances information processing and detection behaviour.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWricha Mishra,Ehsan Kheradpezhouh,Ehsan ArabzadehPubMed 38168628
- iScience 27:1086702023Inhibition of 7α,26-dihydroxycholesterol biosynthesis promotes midbrain dopaminergic neuron development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJames Hennegan,Aled H Bryant,Lauren Griffiths,Matthieu Trigano,Oliver J M Bartley,Joanna J Bartlett,Carys Minahan,Willy Antoni Abreu de Oliveira,Eylan Yutuc,Sotirios Ntikas,Christos S Bartsocas,Margarita Markouri,Eleni Antoniadou,Ioanna Laina,Owain W Howell,Meng Li,Yuqin Wang,William J Griffiths,Emma L Lane,Mariah J Lelos,Spyridon TheofilopoulosPubMed 38155767
- Journal of inflammation (London, England) 20:432023Circadian dependency of microglial heme oxygenase-1 expression and inflammation determine neuronal injury in hemorrhagic stroke.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuise Henrich,Iva Kiessling,Matti Steimer,Sibylle Frase,Sandra Kaiser,Nils SchallnerPubMed 38104143
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 38:e233472023Fatty acid binding protein 4 (FABP4) induces chondrocyte degeneration via activation of the NF-κb signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChaofan Zhang,Yiming Lin,Hongyan Li,Hongxin Hu,Yongfa Chen,Ying Huang,Zida Huang,Xinyu Fang,Wenming Zhang,Yunzhi LinPubMed 38095503
- Bioengineering (Basel, Switzerland) 10:2023Anti-Apoptosis Therapy for Meniscal Avascular Zone Repair: A Proof-of-Concept Study in a Lapine Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenqiang Yan,Yue Wu,Fengyuan Zhao,Ruilan Dai,Yunan Zhou,Dingge Liu,Jin Cheng,Xiaoqing Hu,Yingfang AoPubMed 38136013
- Cell 186:5910-5924.e172023A tridimensional atlas of the developing human head.Applications:IHC - WmtReactive species:HumanRaphael Blain,Gérard Couly,Eimad Shotar,Joséphine Blévinal,Maryne Toupin,Anais Favre,Ali Abjaghou,Megumi Inoue,Edwin Hernández-Garzón,Frédéric Clarençon,Frédéric Chalmel,Séverine Mazaud-Guittot,Paolo Giacobini,Yorick Gitton,Alain ChédotalPubMed 38070509
- Stem cell research & therapy 14:3492023A 3D "sandwich" co-culture system with vascular niche supports mouse embryo development from E3.5 to E7.5 in vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunjun Xu,Linye Zhang,Zihui Ye,Binwen Chang,Zheng Tu,Xuguang Du,Xi Wen,Yili TengPubMed 38072932
- Journal of neuroinflammation 20:2852023Single-cell RNA sequencing unveils Lrg1's role in cerebral ischemia‒reperfusion injury by modulating various cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhaohui Ruan,Guosheng Cao,Yisong Qian,Longsheng Fu,Jinfang Hu,Tiantian Xu,Yaoqi Wu,Yanni LvPubMed 38037097
Key facts
- Host species
- Donkey
- Target species
- Goat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alexa Fluor® 488
- Excitation/Emission
- Ex: 495nm, Em: 519nm
- Applications
- ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000 - 1/4000 | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200 - 1/1000 | Notes - |
Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G
Recommended products
Donkey anti-goat IgG H&L (Alexa Fluor® 488) is a secondary antibody with a maximum absorption wavelength of 495nm and a maximum emission wavelength of 519nm. Ideal for fluorescent cell and tissue imaging. Suitable for ICC/IF, IHC, flow cytometry and ELISA.
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 360 publications
Key facts
- Description
- Donkey Anti-Goat IgG H&L (Alexa Fluor® 488)
- Host species
- Donkey
- Target species
- Goat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alexa Fluor® 488
- Excitation/Emission
- Ex: 495nm, Em: 519nm
- Applications
- ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
Fluorochrome chart - a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.
When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies , designed to deliver precise and reliable results.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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10 product images
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling MAP2 with Anti-MAP2 antibody [EPR19691] - Goat IgG (Chimeric) ab302487 at 1/100 (10.04 ug/ml) dilution, followed by ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/mL dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neuronConfocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection is observed. Anti-MAP2 antibody [EPR19691] - Neuronal Marker ab183830 Anti-MAP2 rabbit monoclonal antibody was used to counterstain tubulin at 1/1000 1ug/mL dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) at 1/1000 2 ug/mL dilution.
Flow Cytometry - Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
Overlay histogram showing Jurkat cells stained Goat polyclonal to Ikaros (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal donkey serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Goat polyclonal to Ikaros, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 donkey anti-goat IgG (H&L) (ab150129) at 1/4000 dilution for 30 min at 22°C. Isotype control antibody (black line) was goat IgG (polyclonal) (Goat IgG, polyclonal - Isotype Control ab37373, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
ICC/IF image of Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, 1μg/ml) overnight at +4°C. ab98800, goat anti-mouse IgG, was then added as a secondary bridging antibody, at 1/250 dilution for 1h. ab150129 Alexa Fluor® 488 donkey anti-goat IgG (H+L) (shown in green) was then used at 1μg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling MAP2 with Anti-MAP2 antibody [EPR19691] - Goat IgG (Chimeric) ab302487 at 1/100 (10.04 ug/ml) dilution, followed by ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/mL dilution (Green). Confocal image showing cytoplasmic staining in rat primary neuronConfocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection is observed. Anti-MAP2 antibody [EPR19691] - Neuronal Marker ab183830 Anti-MAP2 rabbit monoclonal antibody was used to counterstain tubulin at 1/1000 1ug/mL dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) at 1/1000 2 ug/mL dilution.
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling GFAP with Anti-GFAP antibody [EPR1034Y] - Goat IgG (Chimeric) ab302644 at 1/250 (3.992 ug/ml) dilution, followed by ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) antibody at 1/500 4ug/ml dilution (Green). Confocal image showing cytoplasmic staining in mouse primary astrocytesConfocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection. is observed. ab10062 Anti-GFAP mouse monoclonal antibody was used to counterstain tubulin at 1/50 10ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) at 1/500 4ug/ml dilution.
Immunohistochemistry (Frozen sections) - Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum (fresh) tissue labeling GFAP with Anti-GFAP antibody [EPR1034Y] - Goat IgG (Chimeric) ab302644 at 1/100 (9.98 ug/ml) dilution followed by ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) at 1/1000 2 ug/mL dilution (Green). Positive staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488)at 1/1000 2 ug/mL dilution.
Immunohistochemistry (Frozen sections) - Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum (fresh) tissue labeling GFAP with Anti-GFAP antibody [EPR1034Y] - Goat IgG (Chimeric) ab302644 at 1/100 (9.98 ug/ml) dilution followed by ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) at 1/1000 2 ug/mL dilution (Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488)at 1/1000 2 ug/mL dilution.
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling GFAP with Anti-GFAP antibody [EPR1034Y] - Goat IgG (Chimeric) ab302644 at 1/250 (3.992 ug/ml) dilution, followed by ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) antibody at 1/500 4ug/ml dilution (Green). Confocal image showing cytoplasmic staining in rat primary astrocytesConfocal scanning Z step was set as 0.3 ?m followed by image processing with maximum Z projection. is observed. ab10062 Anti-GFAP mouse monoclonal antibody was used to counterstain tubulin at 1/50 10ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) at 1/500 4ug/ml dilution.
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling non-muscle Myosin IIB/MYH10 with Anti-non-muscle Myosin IIB/MYH10 antibody [EPR22564-23] - Goat IgG (Chimeric) - BSA and Azide free ab300649 at 1/50 (19.56 ug/ml) dilution, followed by ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) antibody at 1/500 4ug/ml dilution (Green). Confocal image showing cytoskeletal staining in NIH/3T3 cell line is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is ab150129 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) at 1/500 4ug/ml dilution.
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