AB150130

Donkey Anti-Goat IgG H&L (Alexa Fluor® 555)

Name: Donkey Anti-Goat IgG (Alexa Fluor® 555) secondary antibody (ab150130) | Abcam
Brand: Abcam Limited
SKU: AB150130
Price: 150 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(76 Publications)

Donkey Anti-Goat IgG H&L (Alexa Fluor® 555) is a secondary antibody with a maximum absorption wavelength of 555nm and a maximum emission wavelength of 565nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.

- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 50 publications

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

3 Images

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 555) (AB150130)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 555) (AB150130)

ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1μg/ml) overnight at +4°C. ab98800 (goat anti-mouse IgG) was then added as a secondary bridging antibody, at 1/250 dilution for 1h. ab150130 Alexa Fluor^® 555 donkey anti-goat IgG (H+L) (yellow) was then used at 2μg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM

The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

ICC/IF

AbReview

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 555) (AB150130)

Immunocytochemsitry/Immunfluorescence analysis of mouse brain organotypic slice labelling GFAP primary with Alexa Fluor® 555 Donkey anti-goat IgG H&L (ab150130). Counter stained with the nuclear marker TO-PRO-3. Clear staining shown with 543nm laser.

This image is courtesy of an Abreview submitted by Kirsty Ireland

Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 555) (AB150130)

Alexa Fluor®

Unknown

Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 555) (AB150130)

Key facts

Host species

Donkey

Target species

Goat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Applications

ELISA, ICC/IF, IHC-P, Flow Cyt, IHC-Fr

applications

Clonality

Polyclonal

Isotype

IgG

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"" } } }

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Product details

This antibody reacts with the heavy and light chains of Goat IgG

Fluorochrome chart- a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.

When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Goat IgG

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Publications (76)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8647 PubMed41028752

2025

Cell-type-specific functionality encoded within the intrinsically disordered regions of OCT4.

Applications

Unspecified application

Species

Unspecified reactive species

Burak Ozkan,Mitzy Rios de Anda,Elisa Hall-Ponsele,Maria Rosa Portero Migueles,Amani Alshaikh,Marta Hanzevacki,Moriyah Naama,Katharine Furlong,Gareth A Roberts,Meryam Beniazza,My Linh Huynh,Michael R O'Dwyer,Sonia Yiakoumi,Christos Spanos,Hazar Yassen,Keisuke Kaji,Hitoshi Niwa,Yosef Buganim,Sally Lowell,Abdenour Soufi

iScience 28:113373 PubMed40970198

2025

Developmental trajectories predict dendritic remodeling after injury.

Applications

Unspecified application

Species

Unspecified reactive species

Joana R F Santos,Chen Li,Lien Andries,Luca Masin,Bram Nuttin,Katja Reinhard,Lieve Moons,Hermann Cuntz,Karl Farrow

iScience 28:112935 PubMed40678520

2025

Human dorsal forebrain organoids show differentiation-state-specific protein secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Zeynep Yentür,Theresa Kagermeier,Kseniia Sarieva,Mohamed A Jarboui,Katharina Becker,Simone Mayer

Materials today. Bio 33:101990 PubMed40605986

2025

In situ vascularization and epithelialization of segmental bioengineered trachea based on marrow-derived stem/progenitor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Sun,Yibo Shan,Shu Pan,Yi Lu,Zhiming Shen,Jianwei Zhu,Lei Yuan,Qi Wang,Wenxuan Chen,Hao Chen,Hongcan Shi

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e05340 PubMed40470686

2025

Large-Scale Bioprinting of Human Epiblast-Like Models Featuring Disc-Shaped Morphogenesis and Gastrulation Events.

Applications

Unspecified application

Species

Unspecified reactive species

Yixue Luo,Liheng Luo,Ling Wang,Shanshan Yang,Hongan Ren,Shaojun Liang,Xiaoyu Wang,Yijun Su,Leqian Yu,Xiaoyue Wang,Mingen Xu,Rui Yao

The Journal of clinical investigation 135: PubMed40454484

2025

Polybromo 1/vimentin axis dictates tumor grade, epithelial-mesenchymal transition, and metastasis in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Munenori Kawai,Akihisa Fukuda,Munehiro Ikeda,Kei Iimori,Kenta Mizukoshi,Kosuke Iwane,Go Yamakawa,Mayuki Omatsu,Mio Namikawa,Makoto Sono,Tomonori Masuda,Yuichi Fukunaga,Munemasa Nagao,Osamu Araki,Takaaki Yoshikawa,Satoshi Ogawa,Yukiko Hiramatsu,Motoyuki Tsuda,Takahisa Maruno,Yuki Nakanishi,Dieter Saur,Tatsuaki Tsuruyama,Toshihiko Masui,Etsuro Hatano,Hiroshi Seno

International journal of molecular sciences 26: PubMed40332752

2025

Introducing a Porcine Inflammatory Ex Vivo Retina Model for Diabetic Retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Agnes Mühle,Sven Schnichels,José Hurst

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2411524 PubMed40013981

2025

NETs-CD44-IL-17A Feedback Loop Drives Th17-Mediated Inflammation in Behçet's Uveitis.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Wu,Kang Ning,Zhaohao Huang,Binyao Chen,Junjie Chen,Yingying Wen,Jian Bu,Han Hong,Qiaorong Chen,Zhuoqi Zhang,Renbing Jia,Wenru Su

Journal of neuroinflammation 22:11 PubMed39833906

2025

Microglial double stranded DNA accumulation induced by DNase II deficiency drives neuroinflammation and neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Ling-Jie Li,Shi-Yu Liang,Xiao-Ying Sun,Jie Zhu,Xiao-Yun Niu,Xiao-Yu Du,Ya-Ru Huang,Rui-Tian Liu

Journal of cellular physiology 240:e31477 PubMed39513231

2024

Dynamic mRNA Stability Buffer Transcriptional Activation During Neuronal Differentiation and Is Regulated by SAMD4A.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Zhou,Sherif Rashad,Daisuke Ando,Yuki Kobayashi,Teiji Tominaga,Kuniyasu Niizuma

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey Anti-Goat IgG H&L (Alexa Fluor® 555)

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 555) (AB150130)

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 555) (AB150130)

Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 555) (AB150130)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (76)

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise