Donkey Anti-Goat IgG H&L (Alexa Fluor® 555) is a secondary antibody with a maximum absorption wavelength of 555nm and a maximum emission wavelength of 565nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 50 publications
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000 | Notes - |
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G
Donkey Anti-Goat IgG H&L (Alexa Fluor® 555) is a secondary antibody with a maximum absorption wavelength of 555nm and a maximum emission wavelength of 565nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 50 publications
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
This antibody reacts with the heavy and light chains of Goat IgG
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Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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ICC/IF image of Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, 1μg/ml) overnight at +4°C. ab98800 (goat anti-mouse IgG) was then added as a secondary bridging antibody, at 1/250 dilution for 1h. ab150130 Alexa Fluor® 555 donkey anti-goat IgG (H+L) (yellow) was then used at 2μg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
Immunocytochemsitry/Immunfluorescence analysis of mouse brain organotypic slice labelling GFAP primary with Alexa Fluor® 555 Donkey anti-goat IgG H&L (ab150130). Counter stained with the nuclear marker TO-PRO-3. Clear staining shown with 543nm laser.
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