Donkey Anti-Goat IgG H&L (Alexa Fluor® 555) is a secondary antibody with a maximum absorption wavelength of 555nm and a maximum emission wavelength of 565nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 50 publications
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Publications
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- Aging and disease 14:2193-22142023Healthy Human Fecal Microbiota Transplantation into Mice Attenuates MPTP-Induced Neurotoxicity via AMPK/SOD2 Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenchao Xie,Mahui Zhang,Yuqi Luo,Dana Jin,Xingfang Guo,Wanlin Yang,Jialing Zheng,Hongfei Zhang,Lu Zhang,Chao Deng,Wenhua Zheng,Eng-King Tan,Kunlin Jin,Shuzhen Zhu,Qing WangPubMed 37199590
- Translational psychiatry 13:3322023Chronic administration of XBD173 ameliorates cognitive deficits and neuropathology via 18 kDa translocator protein (TSPO) in a mouse model of Alzheimer's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArpit Kumar Pradhan,Tatjana Neumüller,Claudia Klug,Severin Fuchs,Martin Schlegel,Markus Ballmann,Katharina Johanna Tartler,Antoine Pianos,Maria Sanchez Garcia,Philippe Liere,Michael Schumacher,Matthias Kreuzer,Rainer Rupprecht,Gerhard RammesPubMed 37891168
- International journal of molecular sciences 24:2023Endothelial Differentiation of Knockout iPSCs Triggers the Establishment of a Specific Gene Expression Signature.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRobin A Pilz,Dariush Skowronek,Lara Mellinger,Sander Bekeschus,Ute Felbor,Matthias RathPubMed 36835400
- Nutrients 15:2022Low-Dose Administration of Cannabigerol Attenuates Inflammation and Fibrosis Associated with Methionine/Choline Deficient Diet-Induced NASH Model via Modulation of Cannabinoid Receptor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNouf Aljobaily,Kelsey Krutsinger,Michael J Viereckl,Raznin Joly,Bridger Menlove,Brexton Cone,Ailaina Suppes,Yuyan HanPubMed 36615835
- Hepatology communications 6:3311-33232022Profiling of cell-free DNA methylation and histone signatures in pediatric NAFLD: A pilot study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDiana Buzova,Maria Rita Braghini,Salvatore Daniele Bianco,Oriana Lo Re,Marco Raffaele,Jan Frohlich,Antoniya Kisheva,Annalisa Crudele,Antonella Mosca,Maria Rita Sartorelli,Clara Balsano,Jan Cerveny,Tommaso Mazza,Anna Alisi,Manlio VinciguerraPubMed 36264206
- The Journal of cell biology 222:2022Convergence of secretory, endosomal, and autophagic routes in trans-Golgi-associated lysosomes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLingjian Zhou,Xutong Xue,Ke Yang,Zhi Feng,Min Liu,José C Pastor-ParejaPubMed 36239631
- iScience 25:1052792022A multi-omics approach to visualize early neuronal differentiation from hESCs in 4D.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAthina Samara,Mari Spildrejorde,Ankush Sharma,Martin Falck,Magnus Leithaug,Stefania Modafferi,Pål Marius Bjørnstad,Ganesh Acharya,Kristina Gervin,Robert Lyle,Ragnhild EskelandPubMed 36304110
- Nature neuroscience 25:1288-12992022GABA facilitates spike propagation through branch points of sensory axons in the spinal cord.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKrishnapriya Hari,Ana M Lucas-Osma,Krista Metz,Shihao Lin,Noah Pardell,David A Roszko,Sophie Black,Anna Minarik,Rahul Singla,Marilee J Stephens,Robert A Pearce,Karim Fouad,Kelvin E Jones,Monica A Gorassini,Keith K Fenrich,Yaqing Li,David J BennettPubMed 36163283
- Nature communications 13:50792022A brainstem monosynaptic excitatory pathway that drives locomotor activities and sympathetic cardiovascular responses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSatoshi Koba,Nao Kumada,Emi Narai,Naoya Kataoka,Kazuhiro Nakamura,Tatsuo WatanabePubMed 36038592
- Arthritis research & therapy 24:1882022Regulation of type I interferon signature by VGLL3 in the fibroblast-like synoviocytes of rheumatoid arthritis patients via targeting the Hippo pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Du,Ran Cui,Na Tian,Miao Chen,Xian-Long Zhang,Sheng-Ming DaiPubMed 35941675
Key facts
- Host species
- Donkey
- Target species
- Goat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alexa Fluor® 555
- Excitation/Emission
- Ex: 555nm, Em: 565nm
- Applications
- ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000 | Notes - |
Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G
Recommended products
Donkey Anti-Goat IgG H&L (Alexa Fluor® 555) is a secondary antibody with a maximum absorption wavelength of 555nm and a maximum emission wavelength of 565nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 50 publications
Key facts
- Description
- Donkey Anti-Goat IgG H&L (Alexa Fluor® 555)
- Host species
- Donkey
- Target species
- Goat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alexa Fluor® 555
- Excitation/Emission
- Ex: 555nm, Em: 565nm
- Applications
- ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
This antibody reacts with the heavy and light chains of Goat IgG
Fluorochrome chart - a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.
When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies , designed to deliver precise and reliable results.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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3 product images
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 555) (ab150130)
ICC/IF image of Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, 1μg/ml) overnight at +4°C. ab98800 (goat anti-mouse IgG) was then added as a secondary bridging antibody, at 1/250 dilution for 1h. ab150130 Alexa Fluor® 555 donkey anti-goat IgG (H+L) (yellow) was then used at 2μg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 555) (ab150130)
This image is courtesy of a customer review submitted by Kirsty IrelandImmunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 555) (ab150130)
Immunocytochemsitry/Immunfluorescence analysis of mouse brain organotypic slice labelling GFAP primary with Alexa Fluor® 555 Donkey anti-goat IgG H&L (ab150130). Counter stained with the nuclear marker TO-PRO-3. Clear staining shown with 543nm laser.
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