AB150132

Donkey Anti-Goat IgG H&L (Alexa Fluor® 594)

Name: Donkey Anti-Goat IgG (Alexa Fluor® 594) secondary antibody (ab150132) | Abcam
Brand: Abcam Limited
SKU: AB150132
Price: 185 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(135 Publications)

Donkey Anti-Goat IgG H&L (Alexa Fluor®594)(ab150132) is a secondary antibody with a maximum absorption wavelength of 590 nm and a maximum emission wavelength of 617 nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.

- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Proven performance: cited in over 130 publications

2 Images

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 594) (AB150132)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 594) (AB150132)

ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1μg/ml) overnight at +4°C. ab98800 (goat anti-mouse IgG) was then added as a secondary bridging antibody, at 1/250 dilution for 1h. ab150132 Alexa Fluor® 594 donkey anti-goat IgG (H+L) (orange) was then used at 1μg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody

Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 594) (AB150132)

Alexa Fluor®

Unknown

Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 594) (AB150132)

Key facts

Host species

Donkey

Target species

Goat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Applications

ICC/IF, IHC-P, ELISA, IHC-Fr, Flow Cyt

applications

Clonality

Polyclonal

Isotype

IgG

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"" } } }

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Product details

This antibody reacts with the heavy and light chains of Goat IgG

Fluorochrome chart- a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.

When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Goat IgG

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Publications (135)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadz1228 PubMed40929275

2025

Bioadaptive liquid-infused multifunctional fibers for long-term neural recording via BDNF stabilization and enhanced neural interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Tae Young Kim,Yeonzu Son,Keun-Young Yook,Dong Gyu Lee,Young Kim,Seo Jung Kim,Kijun Park,Yurim Lee,Tae Kyung Lee,Justin J Chung,Congqi Yang,Seongjun Park,Jungmok Seo

Biomedicines 13: PubMed40722706

2025

Application of Human Epineural Patch (hEP) as a Novel Strategy for Nerve Protection and Enhancement of Regeneration After Nerve Crush Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Katarzyna Kozlowska,Weronika Radecka,Sonia Brodowska,Lucile Chambily,Dominika Kuc,Amber Lopez,Maria Siemionow

Journal of neuroinflammation 22:168 PubMed40581647

2025

Exercise-induced irisin ameliorates cognitive impairment following chronic cerebral hypoperfusion by suppressing neuroinflammation and hippocampal neuronal apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Weiping Xiao,Yibing Yang,Lu Bai,Peixuan Yang,Runze Li,Daizhi Yang,Fanying Li,Lingzhi Quan,Qiupeng Liang,Yan Yan,Tiewei Qi,Feng Liang

Nature communications 16:4792 PubMed40410171

2025

RNA-binding proteins DND1 and NANOS3 cooperatively suppress the entry of germ cell lineage.

Applications

Unspecified application

Species

Unspecified reactive species

Ziqi Wang,Honglin Yu,Zhaoyu Gu,Xiaohui Shi,Jiayue Ma,Qizhe Shao,Yao Yao,Shuo Yao,Yan Xu,Yashi Gu,Jiayue Dai,Qi Liu,Jingyan Shi,Rujie Qi,Yue Jin,Yuqian Liu,Xinchen Shen,Wenwen Huang,Heng-Jia Liu,Min Jin,Wanlu Liu,Matthew Brook,Di Chen

Journal of orthopaedic translation 52:97-115 PubMed40275883

2025

PDGF-BB improves cortical bone quality through restoring the osteogenic microenvironment in the steroid-associated osteonecrosis of rabbits.

Applications

Unspecified application

Species

Unspecified reactive species

Huijuan Cao,Keda Shi,Jing Long,Yanzhi Liu,Xiangbo Meng,Cuishan Huang,Jie Hao,Lingli Li,Yiqing Zhao,Tianluo Ye,Yuxiao Lai,Ling Qin,Xinluan Wang

Materials today. Bio 32:101690 PubMed40225136

2025

Peptide loaded self-healing hydrogel promotes diabetic skin wound healing through macrophage orchestration and inflammation inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Ziyi Lu,Kaijia Tan,Shuwen Xiang,Yuchen Zhang,Fangliang Luo,Xingdan Liu,Xiaoli Zhao,Liping Ouyang

Frontiers in molecular neuroscience 18:1552819 PubMed40206188

2025

The restoration of REST inhibits reactivity of Down syndrome iPSC-derived astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Tan Huang,Sharida Fakurazi,Pike-See Cheah,King-Hwa Ling

iScience 28:112115 PubMed40201125

2025

Spatiotemporal proteomic and transcriptomic landscape of DAT+ dopaminergic neurons development and function.

Applications

Unspecified application

Species

Unspecified reactive species

Heyu Zhao,Peipei Chen,Xia Gao,Zhili Huang,Pengyuan Yang,Huali Shen

Archivum immunologiae et therapiae experimentalis 73: PubMed40146977

2025

Protective Effect of the Human Epineural Patch Application after Sciatic Nerve Crush Injury Followed by Nerve Transection and End-to-End Repair.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Siemionow,Weronika Radecka,Katarzyna Kozlowska,Lucile Chambily,Sonia Brodowska,Dominika Kuc,Gabrielle Filipek,Katarzyna Budzynska

Brain structure & function 230:42 PubMed40064689

2025

The Hippo effector TEAD1 regulates postnatal murine cerebellar development.

Applications

Unspecified application

Species

Unspecified reactive species

Cooper Atterton,Alexandra Pelenyi,Justin Jones,Laura Currey,Majd Al-Khalily,Lucinda Wright,Mikki Doonan,David Knight,Nyoman D Kurniawan,Shaun Walters,Stefan Thor,Michael Piper

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey Anti-Goat IgG H&L (Alexa Fluor® 594)

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 594) (AB150132)

Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 594) (AB150132)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (135)

Product promise