AB150131

Donkey Anti-Goat IgG H&L (Alexa Fluor® 647)

Name: Donkey Anti-Goat IgG (Alexa Fluor® 647) secondary antibody (ab150131) | Abcam
Brand: Abcam Limited
SKU: AB150131
Price: 130 USD
Availability: InStock

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(95 Publications)

Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) is a secondary antibody with a maximum absorption wavelength of 650nm and a maximum emission wavelength of 665nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.

- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 90 publications

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

2 Images

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) (AB150131)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) (AB150131)

ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1μg/ml) overnight at +4°C. ab98800, goat anti-mouse IgG, was then added as a secondary bridging antibody, at 1/250 dilution for 1h. ab150131 Alexa Fluor® 647 donkey anti-goat IgG (H+L) (shown in red) was then used at 1μg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) (AB150131)

Alexa Fluor®

Unknown

Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) (AB150131)

Key facts

Host species

Donkey

Target species

Goat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Applications

ICC/IF, ELISA, Flow Cyt, IHC-P, IHC-Fr

applications

Clonality

Polyclonal

Isotype

IgG

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"" } } }

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Product details

This antibody reacts with the heavy and light chains of Goat IgG

Fluorochrome chart- a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.

When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Goat IgG

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Publications (95)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 9:e22614 PubMed38107325

2023

Decoy peptides effectively inhibit the binding of SARS-CoV-2 to ACE2 on oral epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lai-Keng Loi,Cheng-Chieh Yang,Yu-Cheng Lin,Yee-Fun Su,Yi-Chen Juan,Yi-Hsin Chen,Hsiu-Chuan Chang

Frontiers in nanotechnology 5:1220514 PubMed37954478

2023

Epidermal growth factor alters silica nanoparticle uptake and improves gold-nanoparticle-mediated gene silencing in A549 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Eva Susnik,Amelie Bazzoni,Patricia Taladriz-Blanco,Sandor Balog,Aura Maria Moreno-Echeverri,Christina Glaubitz,Beatriz Brito Oliveira,Daniela Ferreira,Pedro Viana Baptista,Alke Petri-Fink,Barbara Rothen-Rutishauser

Journal of neuroinflammation 20:240 PubMed37864249

2023

Intermittent hypoxia therapy ameliorates beta-amyloid pathology via TFEB-mediated autophagy in murine Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Xueting Wang,Yuqi Xie,Guijuan Chen,Yapeng Lu,Dan Wang,Li Zhu

Scientific reports 13:17874 PubMed37857701

2023

Neuroprotective potential of intranasally delivered L-myc immortalized human neural stem cells in female rats after a controlled cortical impact injury.

Applications

Unspecified application

Species

Unspecified reactive species

Mari Amirbekyan,Vikram Adhikarla,Jeffrey P Cheng,Eleni H Moschonas,Corina O Bondi,Russell C Rockne,Anthony E Kline,Margarita Gutova

JCI insight 8: PubMed37811653

2023

RhoA vesicle trafficking-mediated transglutaminase 2 membrane translocation promotes IgA1 mesangial deposition in IgA nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Zhong Zhong,Zhijian Li,Yanjie Li,Lanping Jiang,Qingyu Kong,Wei Chen,Shaozhen Feng

Signal transduction and targeted therapy 8:342 PubMed37691058

2023

Temporospatial inhibition of Erk signaling is required for lymphatic valve formation.

Applications

Unspecified application

Species

Unspecified reactive species

Yaping Meng,Tong Lv,Junfeng Zhang,Weimin Shen,Lifang Li,Yaqi Li,Xin Liu,Xing Lei,Xuguang Lin,Hanfang Xu,Anming Meng,Shunji Jia

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2303291 PubMed37553778

2023

Lepr-Expressing PDLSCs Contribute to Periodontal Homeostasis and Respond to Mechanical Force by Piezo1.

Applications

Unspecified application

Species

Unspecified reactive species

Danting Zhang,Weimin Lin,Shuang Jiang,Peng Deng,Linfeng Liu,Qian Wang,Rui Sheng,Hui Sophie Shu,Lijun Wang,Weiguo Zou,Bo O Zhou,Junjun Jing,Ling Ye,Bo Yu,Shiwen Zhang,Quan Yuan

Biology 12: PubMed37508377

2023

Corin Deficiency Diminishes Intestinal Sodium Excretion in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiabing Gu,Kun Wang,Wenguo Li,Meiling He,Tiantian Zhou,Meng Liu,Qingyu Wu,Ningzheng Dong

Science advances 9:eadf9790 PubMed37327343

2023

Cell competition for neuron-derived trophic factor controls the turnover and lifespan of microglia.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Yu,Haoyue Kuang,Xiaohai Wu,Ying Huang,Jianzhong Wang,Zilong Wen

Nature communications 14:3074 PubMed37244912

2023

Single-cell transcriptomics reveals immune suppression and cell states predictive of patient outcomes in rhabdomyosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jeff DeMartino,Michael T Meister,Lindy L Visser,Mariël Brok,Marian J A Groot Koerkamp,Amber K L Wezenaar,Laura S Hiemcke-Jiwa,Terezinha de Souza,Johannes H M Merks,Anne C Rios,Frank C P Holstege,Thanasis Margaritis,Jarno Drost

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey Anti-Goat IgG H&L (Alexa Fluor® 647)

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) (AB150131)

Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) (AB150131)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (95)

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise