Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) is a preadsorbed secondary antibody with a maximum absorption wavelength of 650nm and a maximum emission wavelength of 665nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.
- Preadsorbed to prevent cross-reactivity and reduce background staining
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 60 publications
Images
Publications
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- Nature 625:126-1332023Cytoneme-mediated transport of active Wnt5b-Ror2 complexes in zebrafish.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChengting Zhang,Lucy Brunt,Yosuke Ono,Sally Rogers,Steffen ScholppPubMed 38123680
- Cell 186:5910-5924.e172023A tridimensional atlas of the developing human head.Applications:IHC - WmtReactive species:HumanRaphael Blain,Gérard Couly,Eimad Shotar,Joséphine Blévinal,Maryne Toupin,Anais Favre,Ali Abjaghou,Megumi Inoue,Edwin Hernández-Garzón,Frédéric Clarençon,Frédéric Chalmel,Séverine Mazaud-Guittot,Paolo Giacobini,Yorick Gitton,Alain ChédotalPubMed 38070509
- Psychopharmacology 241:687-6982023Liraglutide ameliorates delirium-like behaviors of aged mice undergoing cardiac surgery by mitigating microglia activation via promoting mitophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin Jia,Xin Lv,Tong Zhu,Jin-Chun Shen,Wen-Xue Liu,Jian-Jun YangPubMed 37968531
- Acta neuropathologica communications 11:1612023Determinants of astrocytic pathology in stem cell models of primary tauopathies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKimberly L Fiock,Jordan N Hook,Marco M HeftiPubMed 37803326
- iScience 26:1072012023Targeting endometrial inflammation in intrauterine adhesion ameliorates endometrial fibrosis by priming MSCs to secrete C1INH.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSimin Yao,Zhenhua Zhou,Limin Wang,Haining Lv,Dan Liu,Qi Zhu,Xiwen Zhang,Guangfeng Zhao,Yali HuPubMed 37456855
- Journal of neuroinflammation 20:1372023Microglia-derived TNF-α contributes to RVLM neuronal mitochondrial dysfunction via blocking the AMPK-Sirt3 pathway in stress-induced hypertension.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLinping Wang,Tianfeng Liu,Xueping Wang,Lei Tong,Gaojun Chen,Shumin Zhou,Haili Zhang,Haisheng Liu,Wen Lu,Guohua Wang,Shuai Zhang,Dongshu DuPubMed 37264405
- Open medicine (Warsaw, Poland) 18:202306502023UHRF1-induced connexin26 methylation is involved in hearing damage triggered by intermittent hypoxia in neonatal rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXingang Zhang,Jishan Zheng,Huiqing Xu,Zhaoxin MaPubMed 36865496
- International journal of oncology 62:2023Lactate secreted via MCT4 from bone‑colonizing breast cancer excites sensory neurons via GPR81.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTatsuo Okui,Masahiro Hiasa,Kazuaki Hasegawa,Tomoya Nakamura,Kisho Ono,Soichiro Ibaragi,Takahiro Kanno,Akira Sasaki,Toshiyuki YonedaPubMed 36799150
- Biomolecules and biomedicine 23:161-1692023Prognostic and predictive significance of VEGF, CD31, and Ang-1 in patients with metastatic clear cell renal cell carcinoma treated with first-line sunitinib.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarija Kraljević,Inga Marijanović,Maja Barbarić,Emir Sokolović,Merima Bukva,Timur Cerić,Teo BuhovacPubMed 35674770
- Science immunology 8:eade27982023Class switch toward noninflammatory, spike-specific IgG4 antibodies after repeated SARS-CoV-2 mRNA vaccination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPascal Irrgang,Juliane Gerling,Katharina Kocher,Dennis Lapuente,Philipp Steininger,Katharina Habenicht,Monika Wytopil,Stephanie Beileke,Simon Schäfer,Jahn Zhong,George Ssebyatika,Thomas Krey,Valeria Falcone,Christine Schülein,Antonia Sophia Peter,Krystelle Nganou-Makamdop,Hartmut Hengel,Jürgen Held,Christian Bogdan,Klaus Überla,Kilian Schober,Thomas H Winkler,Matthias TenbuschPubMed 36548397
Key facts
- Host species
- Donkey
- Target species
- Goat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Rabbit, Chicken, Human, Pig, Rat
- Conjugation
- Alexa Fluor® 647
- Excitation/Emission
- Ex: 650nm, Em: 665nm
- Applications
- ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000 | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G
Recommended products
Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) is a preadsorbed secondary antibody with a maximum absorption wavelength of 650nm and a maximum emission wavelength of 665nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.
- Preadsorbed to prevent cross-reactivity and reduce background staining
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 60 publications
Key facts
- Description
- Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed
- Host species
- Donkey
- Target species
- Goat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Rabbit, Chicken, Human, Pig, Rat
- Conjugation
- Alexa Fluor® 647
- Excitation/Emission
- Ex: 650nm, Em: 665nm
- Applications
- ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF
- Clonality
- Polyclonal
- Pre-adsorbed
- Yes
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with goat IgG and with light chains common to other goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to chicken, human, mouse, pig, rabbit and rat IgG was detected. This antibody may cross react with IgG from other species.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
Antiserum was cross adsorbed using chicken, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive Antibodies. The antibody to goat IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
This antibody reacts with the heavy and light chains of Goat IgG
Fluorochrome chart - a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.
When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies , designed to deliver precise and reliable results.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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12 product images
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150135)
ICC/IF image of Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, 1µg/ml) overnight at +4°C. ab98800, goat anti-mouse IgG, was then added as a secondary bridging antibody, at 1/250 dilution for 1h. ab150135 Alexa Fluor® 647 donkey anti-goat IgG (H+L) (red) was then used at 2µg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150135)
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150135)
This image was produced using Anti-alpha smooth muscle Actin antibody [EPR5368] – Goat IgG (Chimeric) – BSA and Azide Free ab320059, the same antibody clone but in a different formulation.
Immunofluorescence staining of ACTA2 in sections of formalin-fixed paraffin-embedded human colon (positive) and human brain (negative)*.
Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with Anti-alpha smooth muscle Actin antibody [EPR5368] – Goat IgG (Chimeric) – BSA and Azide Free ab320059 at 1/500 dilution and then incubated for 1 hour with ab150135 Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed at 1/1000 dilution (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150135)
Immunofluorescence staining of Tyrosine Hydroxylase in sections of formalin-fixed paraffin-embedded mouse brain (positive) and mouse liver (negative).
Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with Anti-Tyrosine Hydroxylase antibody [EP1532Y] – Goat IgG (Chimeric) – BSA and Azide Free ab317795 at 1/500 dilution, and then incubated for 1 hour with ab150135 Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150135)
Immunofluorescence staining of Tyrosine Hydroxylase in sections of formalin-fixed paraffin-embedded rat brain (positive) and rat liver (negative).
Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with Anti-Tyrosine Hydroxylase antibody [EP1532Y] – Goat IgG (Chimeric) – BSA and Azide Free ab317795 at 1/100 dilution, and then incubated for 1 hour with ab150135 Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150135)
Immunofluorescence staining of PSD95 in sections of formalin-fixed paraffin-embedded rat brain (positive) and rat liver (negative).
Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with Anti-PSD95 antibody [EPR23124-118] – Goat IgG (Chimeric) – BSA and Azide Free ab317803 at 1/100 dilution, and then incubated for 1 hour with ab150135 Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed at 1/1000 dilution (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150135)
Immunofluorescence staining of ACTA2 in sections of formalin-fixed paraffin-embedded rat stomach (positive) and rat brain (negative).
Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with Anti-alpha smooth muscle Actin antibody [EPR5368] – Goat IgG (Chimeric) – BSA and Azide Free ab320059 at 1/500 dilution and then incubated for 1 hour with ab150135 Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed at 1/1000 dilution (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150135)
Immunofluorescence staining of beta actin in sections of formalin-fixed paraffin-embedded mouse stomach (positive) and mouse liver (low expressor).
Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with Anti-beta Actin antibody [SP124] – Goat IgG (Chimeric) – BSA and Azide Free ab317804 at 1/500 dilution, and then incubated for 1 hour with ab150135 Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed at 1/1000 dilution (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150135)
Immunofluorescence staining of beta actin in sections of formalin-fixed paraffin-embedded rat stomach (positive) and rat liver (low expressor).
Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with Anti-beta Actin antibody [SP124] – Goat IgG (Chimeric) – BSA and Azide Free ab317804 at 1/500 dilution, and then incubated for 1 hour with ab150135 Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed at 1/1000 dilution (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150135)
Immunofluorescence staining of beta actin in sections of formalin-fixed paraffin-embedded human colon (positive) and human liver (low expressor).
Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with Anti-beta Actin antibody [SP124] – Goat IgG (Chimeric) – BSA and Azide Free ab317804 at 1/100 dilution, and then incubated for 1 hour with ab150135 Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed at 1/1000 dilution (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150135)
Immunofluorescence staining of PSD95 in sections of formalin-fixed paraffin-embedded mouse brain (positive) and mouse liver (negative).
Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with Anti-PSD95 antibody [EPR23124-118] – Goat IgG (Chimeric) – BSA and Azide Free ab317803 at 1/100 dilution, and then incubated for 1 hour with ab150135 Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed at 1/1000 dilution (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150135)
Immunofluorescence staining of ACTA2 in sections of formalin-fixed paraffin-embedded mouse stomach (positive) and mouse brain (negative).
Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with Anti-alpha smooth muscle Actin antibody [EPR5368] – Goat IgG (Chimeric) – BSA and Azide Free ab320059 at 1/500 dilution and then incubated for 1 hour with ab150135 Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed at 1/1000 dilution (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
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