Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed (AB150135)

ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) overnight at +4°C. ab98800, goat anti-mouse IgG, was then added as a secondary bridging antibody, at 1/250 dilution for 1h. ab150135 Alexa Fluor® 647 donkey anti-goat IgG (H+L) (red) was then used at 2µg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed (AB150135)

Composite multiplex immunofluorescence staining of APP, PSD95 and Tau staining in a section of formalin-fixed paraffin-embedded human Alzheimer’s brain*.

Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with EDTA (Ph9.0) using retrieval settings of 100°C for 40 minutes. The section was then incubated at room temperature for 1 hour with ab201060 at 1µg/ml dilution (shown in green), ab317793 at 1µg/ml (shown in magenta), and ab317841 at 1µg/ml (shown in yellow). Then incubated for 1 hour with ab150118 Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed 1/1000, ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed 1/1000, and ab150135 Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed 1/1000. Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium ^®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed (AB150135)

This image was produced using ab320059, the same antibody clone but in a different formulation. Immunofluorescence staining of ACTA2 in sections of formalin-fixed paraffin-embedded human colon (positive) and human brain (negative)*. Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with ab320059 at 1/500 dilution and then incubated for 1 hour with ab150135 Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed at 1/1000 dilution (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed (AB150135)

Immunofluorescence staining of beta actin in sections of formalin-fixed paraffin-embedded human colon (positive) and human liver (low expressor). Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with ab317804 at 1/100 dilution, and then incubated for 1 hour with ab150135 Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed at 1/1000 dilution (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times. *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed (AB150135)

Immunofluorescence staining of PSD95 in sections of formalin-fixed paraffin-embedded mouse brain (positive) and mouse liver (negative). Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with ab317803 at 1/100 dilution, and then incubated for 1 hour with ab150135 Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed at 1/1000 dilution (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed (AB150135)

Immunofluorescence staining of beta actin in sections of formalin-fixed paraffin-embedded mouse stomach (positive) and mouse liver (low expressor). Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with ab317804 at 1/500 dilution, and then incubated for 1 hour with ab150135 Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed at 1/1000 dilution (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed (AB150135)

Immunofluorescence staining of Tyrosine Hydroxylase in sections of formalin-fixed paraffin-embedded rat brain (positive) and rat liver (negative). Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with ab317795 at 1/100 dilution, and then incubated for 1 hour with ab150135 Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed (AB150135)

Immunofluorescence staining of PSD95 in sections of formalin-fixed paraffin-embedded rat brain (positive) and rat liver (negative). Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with ab317803 at 1/100 dilution, and then incubated for 1 hour with ab150135 Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed at 1/1000 dilution (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed (AB150135)

Immunofluorescence staining of Tyrosine Hydroxylase in sections of formalin-fixed paraffin-embedded mouse brain (positive) and mouse liver (negative). Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with ab317795 at 1/500 dilution, and then incubated for 1 hour with ab150135 Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed (AB150135)

Immunofluorescence staining of beta actin in sections of formalin-fixed paraffin-embedded rat stomach (positive) and rat liver (low expressor). Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with ab317804 at 1/500 dilution, and then incubated for 1 hour with ab150135 Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed at 1/1000 dilution (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed (AB150135)

Immunofluorescence staining of ACTA2 in sections of formalin-fixed paraffin-embedded mouse stomach (positive) and mouse brain (negative).Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with ab320059 at 1/500 dilution and then incubated for 1 hour with ab150135 Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed at 1/1000 dilution (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions antibody concentrations and incubation times.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed (AB150135)

Immunofluorescence staining of ACTA2 in sections of formalin-fixed paraffin-embedded rat stomach (positive) and rat brain (negative).Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with ab320059 at 1/500 dilution and then incubated for 1 hour with ab150135 Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed at 1/1000 dilution (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions antibody concentrations and incubation times.

• Alexa Fluor®

Unknown

Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) preadsorbed (AB150135)