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AB175776

Donkey Anti-Goat IgG H&L (Alexa Fluor® 680)

5

(1 Review)

|

(9 Publications)

Suitable for WB. Cited in 9 publications.
1 Images
Western blot - Donkey Anti-Goat IgG H&L (Alexa Fluor® 680) (AB175776)
  • WB

Lab

Western blot - Donkey Anti-Goat IgG H&L (Alexa Fluor® 680) (AB175776)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab9483 overnight at 4°C. Antibody binding was detected using ab175776 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-GAPDH antibody - Loading Control (<a href='/en-us/products/primary-antibodies/gapdh-antibody-loading-control-ab9483'>ab9483</a>) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) at 10 µg

Lane 2:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) at 5 µg

Lane 3:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) at 2 µg

Lane 4:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) at 1 µg

Lane 5:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) at 0.5 µg

Secondary

All lanes:

Western blot - Donkey Anti-Goat IgG H&L (Alexa Fluor® 680) (ab175776) at 1/10000 dilution

Predicted band size: 36 kDa

Observed band size: 37 kDa

false

Key facts

Host species

Donkey

Target species

Goat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Alexa Fluor® 680

Excitation/Emission

Ex: 679nm, Em: 702nm

Applications

WB

applications

Clonality

Polyclonal

Isotype

IgG

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000", "notes":"<p></p>" } } }

Product details

We batch test Donkey Anti-Goat IgG H&L (Alexa Fluor® 680), ab175776 in fluorescent WB. Although we don't batch test for ICC, ELISA, IHC-Fr or Flow cytometry customers have had success using Donkey Anti-Goat IgG H&L (Alexa Fluor® 680), ab175776 in these applications.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

Product protocols

Publications (9)

Recent publications for all applications. Explore the full list and refine your search

The FEBS journal 292:3360-3377 PubMed39673076

2024

Targeted protein degradation of PDE4 shortforms by a novel proteolysis targeting chimera.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Yan Sin,Aoife Giblin,Aleksandra Judina,Punchita Rujirachaivej,Laura G Corral,Eliza Glennon,Zhi Xian Tai,Tian Feng,Eduardo Torres,Alina Zorn,Julia Gorelik,Elka Kyurkchieva,Pa Thai Yenchitsomanus,Cathy Swindlehurst,Kyle Chan,David Stirling,George S Baillie

International journal of molecular sciences 24: PubMed37686439

2023

Novel Correlation between TGF-β1/-β3 and Hormone Receptors in the Human Corneal Stroma.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander J Choi,Brenna S Hefley,Sarah E Nicholas,Rebecca L Cunningham,Dimitrios Karamichos

Frontiers in pharmacology 12:663774 PubMed33967807

2021

Targeting Gremlin 1 Prevents Intestinal Fibrosis Progression by Inhibiting the Fatty Acid Oxidation of Fibroblast Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Yang,Qi-Shan Zeng,Min Zou,Jian Zeng,Jiao Nie,DongFeng Chen,Hua-Tian Gan

Oncogenesis 9:92 PubMed33070167

2020

Aberrant super-enhancer landscape reveals core transcriptional regulatory circuitry in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Te Zhang,Xuming Song,Zeyu Zhang,Qixing Mao,Wenjie Xia,Lin Xu,Feng Jiang,Gaochao Dong

Frontiers in neuroscience 13:554 PubMed31191238

2019

Impact of Sleep-Wake-Associated Neuromodulators and Repetitive Low-Frequency Stimulation on Human iPSC-Derived Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Remi Yokoi,Miho Okabe,Naoki Matsuda,Aoi Odawara,Akihiro Karashima,Ikuro Suzuki

Scientific reports 8:10416 PubMed29991696

2018

Toxicological evaluation of convulsant and anticonvulsant drugs in human induced pluripotent stem cell-derived cortical neuronal networks using an MEA system.

Applications

Unspecified application

Species

Unspecified reactive species

A Odawara,N Matsuda,Y Ishibashi,R Yokoi,I Suzuki

Scientific reports 7:17606 PubMed29242624

2017

Plant-derived compounds strigolactone GR24 and pinosylvin activate SIRT1 and enhance glucose uptake in rat skeletal muscle cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shalem Modi,Nagendra Yaluri,Tarja Kokkola,Markku Laakso

PloS one 12:e0176883 PubMed28453568

2017

Baicalin inhibits biofilm formation, attenuates the quorum sensing-controlled virulence and enhances Pseudomonas aeruginosa clearance in a mouse peritoneal implant infection model.

Applications

WB

Species

Unspecified reactive species

Jing Luo,Biying Dong,Ke Wang,Shuangqi Cai,Tangjuan Liu,Xiaojing Cheng,Danqing Lei,Yanling Chen,Yanan Li,Jinliang Kong,Yiqiang Chen

Scientific reports 6:26181 PubMed27188845

2016

Physiological maturation and drug responses of human induced pluripotent stem cell-derived cortical neuronal networks in long-term culture.

Applications

ICC

Species

Unspecified reactive species

A Odawara,H Katoh,N Matsuda,I Suzuki
View all publications

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