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Suitable for IHC-Fr, Flow Cyt, ICC/IF, IHC-P, ELISA. Cited in 1 publication.

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Images

Western blot - Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) (AB175744), expandable thumbnail
  • Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) (AB175744), expandable thumbnail

Publications

Key facts

Host species
Donkey
Target species
Goat
Target isotype
IgG
Target specificity
Heavy & Light chains
Conjugation
Alexa Fluor® 750
Excitation/Emission
Ex: 749nm, Em: 775nm
Applications
IHC-Fr, Flow Cyt, ICC/IF, IHC-P, ELISA
Clonality
Polyclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Application
IHC-Fr
Reactivity
Reacts
Dilution info
-
Notes

This applicaiton is no longer batch tested

Application
Flow Cyt
Reactivity
Reacts
Dilution info
1/2000
Notes

This applicaiton is no longer batch tested

Application
ICC/IF
Reactivity
Reacts
Dilution info
1/200.00000 - 1/1000.00000
Notes

This applicaiton is no longer batch tested

Application
IHC-P
Reactivity
Reacts
Dilution info
-
Notes

This applicaiton is no longer batch tested

Application
ELISA
Reactivity
Reacts
Dilution info
-
Notes

This applicaiton is no longer batch tested

Alternative names

Recommended products

Suitable for IHC-Fr, Flow Cyt, ICC/IF, IHC-P, ELISA. Cited in 1 publication.

Key facts

Description
Donkey Anti-Goat IgG H&L (Alexa Fluor® 750)
Host species
Donkey
Target species
Goat
Target isotype
IgG
Target specificity
Heavy & Light chains
Conjugation
Alexa Fluor® 750
Excitation/Emission
Ex: 749nm, Em: 775nm
Applications
IHC-Fr, Flow Cyt, ICC/IF, IHC-P, ELISA
Clonality
Polyclonal
Pre-adsorbed
No
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Concentration
Loading...

Properties

Form
Liquid
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Purification technique
Affinity purification Immunogen
Purification notes

This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark

Notes

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) (ab175744), expandable thumbnail

    Western blot - Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) (ab175744)

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% milk blocking buffer before being incubated with Anti-GAPDH antibody - Loading Control ab9483 overnight at 4°C. Antibody binding was detected using ab175744 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

    All lanes: Western blot - Anti-GAPDH antibody - Loading Control (Anti-GAPDH antibody - Loading Control ab9483) at 1 µg/mL

    All lanes: HeLa Whole Cell Lysate at 10 µg

    Secondary

    All lanes: Western blot - Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) (ab175744) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 36 kDa

    Observed band size: 50 kDa

  • Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) (ab175744), expandable thumbnail

    Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) (ab175744)

Downloads

Fluorochrome chart

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com