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AB175744

Donkey Anti-Goat IgG H&L (Alexa Fluor® 750)

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(3 Publications)

Suitable for IHC-Fr, Flow Cyt, ICC/IF, IHC-P, ELISA. Cited in 3 publications.
2 Images
Western blot - Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) (AB175744)
  • WB

Lab

Western blot - Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) (AB175744)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% milk blocking buffer before being incubated with ab9483 overnight at 4°C. Antibody binding was detected using ab175744 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-GAPDH antibody - Loading Control (<a href='/en-us/products/primary-antibodies/gapdh-antibody-loading-control-ab9483'>ab9483</a>) at 1 µg/mL

All lanes:

HeLa Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) (ab175744) at 1/10000 dilution

Predicted band size: 36 kDa

Observed band size: 50 kDa

false

Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) (AB175744)
  • Alexa Fluor®

Unknown

Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) (AB175744)

Key facts

Host species

Donkey

Target species

Goat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Alexa Fluor® 750

Excitation/Emission

Ex: 749nm, Em: 775nm

Applications

Flow Cyt, IHC-Fr, ICC/IF, ELISA, IHC-P

applications

Clonality

Polyclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>This applicaiton is no longer batch tested</p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<p>This applicaiton is no longer batch tested</p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"<p>This applicaiton is no longer batch tested</p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>This applicaiton is no longer batch tested</p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>This applicaiton is no longer batch tested</p>" } } }

Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

Product protocols

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature neuroscience 28:2022-2033 PubMed40826298

2025

FOXP genes regulate Purkinje cell diversity and cerebellar morphogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Nagham Khouri-Farah,Qiuxia Guo,Thomas A Perry,Ryan Dussault,James Y H Li

Nature cell biology 26:825-838 PubMed38605144

2024

A CRISPRi/a screening platform to study cellular nutrient transport in diverse microenvironments.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher Chidley,Alicia M Darnell,Benjamin L Gaudio,Evan C Lien,Anna M Barbeau,Matthew G Vander Heiden,Peter K Sorger

Biological psychiatry 87:1001-1011 PubMed31836175

2019

Ghrelin and Orexin Interact to Increase Meal Size Through a Descending Hippocampus to Hindbrain Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea N Suarez,Clarissa M Liu,Alyssa M Cortella,Emily E Noble,Scott E Kanoski
View all publications

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