Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) is a preadsorbed secondary antibody with a maximum absorption wavelength of 749nm and a maximum emission wavelength of 775nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.
- Preadsorbed to prevent cross-reactivity and reduce background staining
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
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Publications
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- Cell 186:5910-5924.e172023A tridimensional atlas of the developing human head.Applications:IHC - WmtReactive species:HumanRaphael Blain,Gérard Couly,Eimad Shotar,Joséphine Blévinal,Maryne Toupin,Anais Favre,Ali Abjaghou,Megumi Inoue,Edwin Hernández-Garzón,Frédéric Clarençon,Frédéric Chalmel,Séverine Mazaud-Guittot,Paolo Giacobini,Yorick Gitton,Alain ChédotalPubMed 38070509
- Nature :2023A human embryonic limb cell atlas resolved in space and time.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBao Zhang,Peng He,John E G Lawrence,Shuaiyu Wang,Elizabeth Tuck,Brian A Williams,Kenny Roberts,Vitalii Kleshchevnikov,Lira Mamanova,Liam Bolt,Krzysztof Polanski,Tong Li,Rasa Elmentaite,Eirini S Fasouli,Martin Prete,Xiaoling He,Nadav Yayon,Yixi Fu,Hao Yang,Chen Liang,Hui Zhang,Raphael Blain,Alain Chedotal,David R FitzPatrick,Helen Firth,Andrew Dean,Omer Ali Bayraktar,John C Marioni,Roger A Barker,Mekayla A Storer,Barbara J Wold,Hongbo Zhang,Sarah A TeichmannPubMed 38057666
- ACS chemical neuroscience 13:1296-13142022O-GlcNAcase Inhibitor ASN90 is a Multimodal Drug Candidate for Tau and α-Synuclein Proteinopathies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBruno Permanne,Astrid Sand,Solenne Ousson,Maud Nény,Jennifer Hantson,Ryan Schubert,Christoph Wiessner,Anna Quattropani,Dirk BeherPubMed 35357812
- Frontiers in molecular neuroscience 14:6818682021Evaluation of Neuropathological Features in the SOD1-G93A Low Copy Number Transgenic Mouse Model of Amyotrophic Lateral Sclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAgnes Molnar-Kasza,Barbara Hinteregger,Joerg Neddens,Roland Rabl,Stefanie Flunkert,Birgit Hutter-PaierPubMed 34248499
- Frontiers in molecular neuroscience 13:6172292021Quantification of Huntington's Disease Related Markers in the R6/2 Mouse Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEstibaliz Etxeberria-Rekalde,Saioa Alzola-Aldamizetxebarria,Stefanie Flunkert,Isabella Hable,Magdalena Daurer,Joerg Neddens,Birgit Hutter-PaierPubMed 33505246
Key facts
- Host species
- Donkey
- Target species
- Goat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Rat, Rabbit, Chicken, Human, Pig
- Conjugation
- Alexa Fluor® 750
- Excitation/Emission
- Ex: 749nm, Em: 775nm
- Applications
- ICC/IF, ELISA, IHC-P, Flow Cyt, IHC-Fr
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ICC/IF | Reactivity Reacts | Dilution info 1/200.00000 - 1/1000.00000 | Notes This applicaiton is no longer batch tested |
Application ELISA | Reactivity Reacts | Dilution info - | Notes This applicaiton is no longer batch tested |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes This applicaiton is no longer batch tested |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000 | Notes This applicaiton is no longer batch tested |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes This applicaiton is no longer batch tested |
Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G
Recommended products
Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) is a preadsorbed secondary antibody with a maximum absorption wavelength of 749nm and a maximum emission wavelength of 775nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.
- Preadsorbed to prevent cross-reactivity and reduce background staining
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
Key facts
- Description
- Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) preadsorbed
- Host species
- Donkey
- Target species
- Goat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Rat, Rabbit, Chicken, Human, Pig
- Conjugation
- Alexa Fluor® 750
- Excitation/Emission
- Ex: 749nm, Em: 775nm
- Applications
- ICC/IF, ELISA, IHC-P, Flow Cyt, IHC-Fr
- Clonality
- Polyclonal
- Pre-adsorbed
- Yes
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with goat IgG and with light chains common to other goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to chicken, human, mouse, pig, rabbit and rat IgG was detected. This antibody may cross react with IgG from other species.
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
Antiserum was cross adsorbed using chicken, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive Antibodies. The antibody to goat IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
This antibody reacts with the heavy and light chains of Goat IgG
Fluorochrome chart - a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.
When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies , designed to deliver precise and reliable results.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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3 product images
Western blot - Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) preadsorbed (ab175745)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% milk blocking buffer before being incubated with Anti-GAPDH antibody - Loading Control ab9483 overnight at 4°C. Antibody binding was detected using ab175745 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
All lanes: Western blot - Anti-GAPDH antibody - Loading Control (Anti-GAPDH antibody - Loading Control ab9483) at 1 µg/mL
All lanes: HeLa Whole Cell Lysate at 10 µg
SecondaryAll lanes: Western blot - Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) preadsorbed (ab175745) at 1/10000 dilution
Performed under reducing conditions.
Western blot - Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) preadsorbed (ab175745)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% milk blocking buffer before being incubated with Anti-beta Actin antibody - Loading Control ab8229 overnight at 4°C. Antibody binding was detected using ab175745 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
All lanes: Western blot - Anti-beta Actin antibody - Loading Control (Anti-beta Actin antibody - Loading Control ab8229) at 1 µg/mL
All lanes: HeLa Whole Cell Lysate at 10 µg
SecondaryAll lanes: Western blot - Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) preadsorbed (ab175745) at 1/10000 dilution
Performed under reducing conditions.
Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) preadsorbed (ab175745)
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