Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) preadsorbed

• WB

Lab

Western blot - Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) preadsorbed (AB175745)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% milk blocking buffer before being incubated with ab9483 overnight at 4°C. Antibody binding was detected using ab175745 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-GAPDH antibody - Loading Control (<a href='/en-us/products/primary-antibodies/gapdh-antibody-loading-control-ab9483'>ab9483</a>) at 1 µg/mL

All lanes:

HeLa Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) preadsorbed (ab175745) at 1/10000 dilution

false

• WB

Lab

Western blot - Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) preadsorbed (AB175745)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% milk blocking buffer before being incubated with ab8229 overnight at 4°C. Antibody binding was detected using ab175745 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-beta Actin antibody - Loading Control (<a href='/en-us/products/primary-antibodies/beta-actin-antibody-loading-control-ab8229'>ab8229</a>) at 1 µg/mL

All lanes:

HeLa Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) preadsorbed (ab175745) at 1/10000 dilution

false

• Alexa Fluor®

Unknown

Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 750) preadsorbed (AB175745)