AB97107

Donkey Anti-Goat IgG H&L (Alkaline Phosphatase)

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(3 Publications)

Suitable for ICC, WB, ELISA, IHC-P. Cited in 3 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

1 Images

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Western blot - Donkey Anti-Goat IgG H&L (Alkaline Phosphatase) (AB97107)

All lanes : Anti-beta Tubulin antibody - Loading Control (ab21057) at 1 μg/ml and HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 μg per lane.

Secondary
Lanes 1 - 2 : Donkey polyclonal Secondary Antibody to Goat IgG - H&L (AP) (ab97107) at 1/5000 dilution
Lanes 3 - 4 : Donkey polyclonal Secondary Antibody to Goat IgG - H&L (AP) (ab97107) at 1/20000 dilution
Lanes 5 - 6 : Donkey polyclonal Secondary Antibody to Goat IgG - H&L (AP) (ab97107) at 1/50000 dilution

Secondary

All lanes:

Western blot - Donkey Anti-Goat IgG H&L (Alkaline Phosphatase) (ab97107)

true

Exposure time: 8min

Key facts

Host species

Donkey

Target species

Goat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Alkaline Phosphatase

Excitation/Emission

Applications

ICC, ELISA, WB, IHC-P

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/5000 - 1/50000", "notes":"(Chemiluminescent)." }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/5000 - 1/50000", "notes":"(Primary)." }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/100 - 1/1000", "notes":"" } } }

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Product details

Part of the AbExcel range.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: 1.19% HEPES, 0.58% Sodium chloride, 0.2% BSA, 0.0095% Magnesium chloride, 0.00136% Zinc chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Goat IgG

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry : PubMed32917722

2020

Sphingosine prevents binding of SARS-CoV-2 spike to its cellular receptor ACE2.

Applications

Unspecified application

Species

Unspecified reactive species

Michael J Edwards,Katrin Anne Becker,Barbara Gripp,Markus Hoffmann,Simone Keitsch,Barbara Wilker,Matthias Soddemann,Anne Gulbins,Elisa Carpinteiro,Sameer H Patel,Gregory C Wilson,Stefan Pöhlmann,Silke Walter,Klaus Fassbender,Syed A Ahmad,Alexander Carpinteiro,Erich Gulbins

PloS one 13:e0206801 PubMed30395621

2018

Mutant myocilin impacts sarcomere ultrastructure in mouse gastrocnemius muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Jeffrey M Lynch,Andrew J Dolman,Chenying Guo,Katie Dolan,Chuanxi Xiang,Samir Reda,Bing Li,Ganesh Prasanna

The Journal of biological chemistry 293:20137-20156 PubMed30389787

2018

Binding of a glaucoma-associated myocilin variant to the αB-crystallin chaperone impedes protein clearance in trabecular meshwork cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jeffrey M Lynch,Bing Li,Parvaneh Katoli,Chuanxi Xiang,Barrett Leehy,Nalini Rangaswamy,Veronica Saenz-Vash,Y Karen Wang,Hong Lei,Thomas B Nicholson,Erik Meredith,Dennis S Rice,Ganesh Prasanna,Amy Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey Anti-Goat IgG H&L (Alkaline Phosphatase)

Western blot - Donkey Anti-Goat IgG H&L (Alkaline Phosphatase) (AB97107)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (3)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise