Donkey Anti-Goat IgG H&L (Biotin)

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Donkey Anti-Goat IgG H&L (Biotin) (AB6884)

ab6884 used to stain samples of whole heads of embryonic day 15 (E15) mice by Immunohistochemistry (Formalin-fixed, paraffin-embedded sections).

Comparison of four staining methods. The heads were flash-frozen directly in isopentane on dry-ice. Once hardened, they were embedded in O.C.T compound. Frozen sections were postfixed in 4% paraformaldehyde in PBS for 20 minutes and quenched in 1.5% hydrogen peroxide for 30 minutes. Sections were then blocked for 2 hours at room temperature with 5% donkey serum in Tris-buffered saline with 0.1% Triton-X100 and then incubated with a goat anti-Nurr1 primary antibody 1/200 in 1% donkey serum in TBS at 4°C overnight. Biotinylated donkey anti-goat (ab6884) 1/500 in 1% donkey serum in TBS was applied for 2 hours at room temperature and reacted with avidin-biotinylated enzyme complex (ABC) using the Vectastain Elite kit and diaminobenzidene (DAB) according to the manufacturer's instructions. Images were taken directly on the PALM Micr

Image from Dr WZ Wang et al, BMC Mol Biol. 2009 Jul 6;10:69, Fig 2.

• ELISA

Supplier Data

ELISA - Donkey Anti-Goat IgG H&L (Biotin) (AB6884)

ELISA results of ab6884 against purified Goat IgG. Each well was coated in duplicate with 1.0 µg of Goat IgG. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, and TMB substrate.