AB6949

Donkey Anti-Goat IgG H&L (Cy3 ®) preadsorbed

Name: Donkey Anti Goat (IgG) secondary antibody preadsorbed Cy3® (ab6949) | Abcam
Brand: Abcam Limited
SKU: AB6949
Price: 295 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(50 Publications)

Suitable for Flow Cyt, ICC, IHC-Fr, IHC-P, ICC/IF, ELISA, WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 50 publications.

1 Images

Supplier Data

Western blot - Donkey Anti-Goat IgG H&L (Cy3 ®) preadsorbed (AB6949)

Both the heavy and light chains of Goat IgG are observed.

All lanes:

Goat IgG, 50 ng

Secondary

All lanes:

Western blot - Donkey Anti-Goat IgG H&L (Cy3 ®) preadsorbed (ab6949) at 1000 Cells

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Key facts

Host species

Donkey

Target species

Goat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Horse, Guinea pig, Hamster, Cow, Mouse, Rat, Rabbit

Pre-adsorbed

Yes

Conjugation

Cy3®

Excitation/Emission

Ex: 552nm, Em: 565nm

Applications

WB, Flow Cyt, ELISA, ICC/IF, IHC-Fr, ICC, IHC-P

applications

Clonality

Polyclonal

Isotype

IgG

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/5000", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/50000", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000", "notes":"" } } }

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Product details

Cy™ and CyDye™ are registered trademarks of Cytiva.

This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.

Storage buffer

pH: 6.5 Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.87% Sodium chloride, 0.42% Tripotassium orthophosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Goat IgG

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Publications (50)

Recent publications for all applications. Explore the full list and refine your search

Molecular neurodegeneration 20:95 PubMed40883786

2025

Recombinant cathepsins B and L promote α-synuclein clearance and restore lysosomal function in human and murine models with α-synuclein pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Denise Balta,Anish Varghese,Susy Prieto Huarcaya,Alessandro Di Spiezio,André R A Marques,Enes Yağız Akdaş,Doğa Tabakacilar,Alice Drobny,Christian Werner,Wei Xiang,Rebecca Mächtel,Jan Philipp Dobert,Anna Fejtova,Franziska Richter,Melanie Küspert,Philipp Arnold,Paul Saftig,Friederike Zunke

BJOG : an international journal of obstetrics and gynaecology 132:107-119 PubMed39973029

2025

Effects of Gonadotropin-Releasing Hormone Analogues on Ovarian Function and Embryogenesis: A Cyclophosphamide-Induced Mouse Model Study.

Applications

Unspecified application

Species

Unspecified reactive species

Qiwang Lin,Mingzhu Cao,Zijin Xu,He Fei,Yunfeng Jin,Jianqiao Liu,Hua Jiang

Advanced materials (Deerfield Beach, Fla.) 37:e2409942 PubMed39523735

2024

Soft-Actuated Cuff Electrodes with Minimal Contact for Bidirectional Peripheral Interfaces.

Applications

Unspecified application

Species

Unspecified reactive species

Hyunmin Moon,Byungwook Park,Namsun Chou,Ki-Su Park,Sanghoon Lee,Sohee Kim

International journal of biological sciences 20:4532-4550 PubMed39247808

2024

The Essential Role of Angiogenesis in Adenosine 2A Receptor Deficiency-mediated Impairment of Wound Healing Involving c-Ski via the ERK/CREB Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Peng,Renping Xiong,Bo Wang,Xing Chen,Yalei Ning,Yan Zhao,Nan Yang,Jing Zhang,Changhong Li,Yuanguo Zhou,Ping Li

Nature communications 15:438 PubMed38200007

2024

Disparate macrophage responses are linked to infection outcome of Hantan virus in humans or rodents.

Applications

Unspecified application

Species

Unspecified reactive species

Hongwei Ma,Yongheng Yang,Tiejian Nie,Rong Yan,Yue Si,Jing Wei,Mengyun Li,He Liu,Wei Ye,Hui Zhang,Linfeng Cheng,Liang Zhang,Xin Lv,Limin Luo,Zhikai Xu,Xijing Zhang,Yingfeng Lei,Fanglin Zhang

Cell 186:5910-5924.e17 PubMed38070509

2023

A tridimensional atlas of the developing human head.

Applications

IHC - Wmt

Species

Human

Raphael Blain,Gérard Couly,Eimad Shotar,Joséphine Blévinal,Maryne Toupin,Anais Favre,Ali Abjaghou,Megumi Inoue,Edwin Hernández-Garzón,Frédéric Clarençon,Frédéric Chalmel,Séverine Mazaud-Guittot,Paolo Giacobini,Yorick Gitton,Alain Chédotal

Immunity 56:2152-2171.e13 PubMed37582369

2023

Human microglia maturation is underpinned by specific gene regulatory networks.

Applications

Unspecified application

Species

Unspecified reactive species

Claudia Z Han,Rick Z Li,Emily Hansen,Samantha Trescott,Bethany R Fixsen,Celina T Nguyen,Cristina M Mora,Nathanael J Spann,Hunter R Bennett,Olivier Poirion,Justin Buchanan,Anna S Warden,Bing Xia,Johannes C M Schlachetzki,Martina P Pasillas,Sebastian Preissl,Allen Wang,Carolyn O'Connor,Shreya Shriram,Roy Kim,Danielle Schafer,Gabriela Ramirez,Jean Challacombe,Samuel A Anavim,Avalon Johnson,Mihir Gupta,Ian A Glass,Michael L Levy,Sharona Ben Haim,David D Gonda,Louise Laurent,Jennifer F Hughes,David C Page,Mathew Blurton-Jones,Christopher K Glass,Nicole G Coufal

CNS neuroscience & therapeutics 29:2634-2644 PubMed37032645

2023

Anti-neuroinflammation effects of transcutaneous auricular vagus nerve stimulation against depression-like behaviors via hypothalamic α7nAchR/JAK2/STAT3/NF-κB pathway in rats exposed to chronic unpredictable mild stress.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Chen,Yue Zhang,Junying Wang,Shaoyuan Li,Yifei Wang,Zixuan Zhang,Jinling Zhang,Chen Xin,Yu Wang,Peijing Rong

Genes to cells : devoted to molecular & cellular mechanisms 28:111-128 PubMed36504347

2023

The cytosolic lncRNA dutA affects STATa signaling and developmental commitment in Dictyostelium.

Applications

Unspecified application

Species

Unspecified reactive species

Yukika Saga,Yudai Shimoyama,Yoko Yamada,Naoki Morikawa,Takefumi Kawata

Nature biomedical engineering 7:72-84 PubMed36564626

2022

Induction of T-helper-17-cell-mediated anti-tumour immunity by pathogen-mimicking polymer nanoparticles.

Applications

Unspecified application

Species

Unspecified reactive species

Sejin Son,Jutaek Nam,April S Kim,Jinsung Ahn,Kyung Soo Park,May Thazin Phoo,Brett Sherren,Weiping Zou,Soo-Hong Lee,Omid C Farokhzad,Jinjun Shi,James J Moon

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey Anti-Goat IgG H&L (Cy3 ®) preadsorbed

Western blot - Donkey Anti-Goat IgG H&L (Cy3 ®) preadsorbed (AB6949)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Complementary Products

Secondary Antibodies

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (50)

Product promise