Suitable for WB, Flow Cyt, ICC, IHC-Fr, IHC-P, ICC/IF, ELISA. Preadsorbed to minimise non-specific binding and high background staining. Cited in 42 publications.
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- iScience 27:1086702023Inhibition of 7α,26-dihydroxycholesterol biosynthesis promotes midbrain dopaminergic neuron development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJames Hennegan,Aled H Bryant,Lauren Griffiths,Matthieu Trigano,Oliver J M Bartley,Joanna J Bartlett,Carys Minahan,Willy Antoni Abreu de Oliveira,Eylan Yutuc,Sotirios Ntikas,Christos S Bartsocas,Margarita Markouri,Eleni Antoniadou,Ioanna Laina,Owain W Howell,Meng Li,Yuqin Wang,William J Griffiths,Emma L Lane,Mariah J Lelos,Spyridon TheofilopoulosPubMed 38155767
- Clinical rheumatology 42:1327-13382023Alterations of CD8 T cells in the blood and salivary glands of patients with primary Sjögren's syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongxia Li,Yaxin Zhou,Pengyu Wang,Yafei Wang,Yuan Feng,Yan Zhang,Zhenbiao WuPubMed 36609932
- Frontiers in physiology 13:9110972022Feeding, Flight Behavior, and Infection With Malaria Parasites are Altered by Ingestion of Serotonin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna M Briggs,Malayna G Hambly,Raquel M Simão-Gurge,Sarah M Garrison,Zainab Khaku,Grace Van Susteren,Edwin E Lewis,Jeffrey A Riffell,Shirley LuckhartPubMed 35747317
- Cells 11:2022Ex Vivo Perfusion Using a Mathematical Modeled, Controlled Gas Exchange Self-Contained Bioreactor Can Maintain a Mouse Kidney for Seven Days.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNatalie Won,Jorge Castillo-Prado,Xinzhu Tan,John Ford,David Heath,Laura Ioana Mazilescu,Markus Selzner,Ian M RogersPubMed 35681517
- iScience 25:1042682022Differing impact of phosphoglycerate mutase 1-deficiency on brown and white adipose tissue.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYohko Yoshida,Ippei Shimizu,Yung-Ting Hsiao,Masayoshi Suda,Goro Katsuumi,Masahide Seki,Yutaka Suzuki,Shujiro Okuda,Tomoyoshi Soga,Tohru MinaminoPubMed 35521515
- Science advances 8:eabm11402022LAMP2A regulates the loading of proteins into exosomes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoão Vasco Ferreira,Ana da Rosa Soares,José Ramalho,Catarina Máximo Carvalho,Maria Helena Cardoso,Petra Pintado,Ana Sofia Carvalho,Hans Christian Beck,Rune Matthiesen,Mónica Zuzarte,Henrique Girão,Guillaume van Niel,Paulo PereiraPubMed 35333565
- Bioactive materials 14:206-2182022Promotion of right ventricular outflow tract reconstruction using a novel cardiac patch incorporated with hypoxia-pretreated urine-derived stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLong-Mei Zhao,Long Wang,Wen-Qian Zhang,Rui Wang,Xiu-Zhen Zhang,Xiong-Xin Lei,Yan Liang,Yu-Ting Song,Qing-Yi Zhang,Ke Lin,Hui-Qi XiePubMed 35310356
- Journal of inflammation research 14:4329-43452021Inhibition of MALT1 Alleviates Spinal Ischemia/Reperfusion Injury-Induced Neuroinflammation by Modulating Glial Endoplasmic Reticulum Stress in Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShutian Zhang,Yufeng Yan,Yongze Wang,Zhaodong Sun,Chengzhi Han,Xinyi Qian,Xiaorong Ren,Yi Feng,Jian Cai,Chunmei XiaPubMed 34511971
- Journal of molecular medicine (Berlin, Germany) 99:1605-16212021The parasite-derived peptide FhHDM-1 activates the PI3K/Akt pathway to prevent cytokine-induced apoptosis of β-cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesInah Camaya,Tsz Y Mok,Maria Lund,Joyce To,Nady Braidy,Mark W Robinson,Jerran Santos,Bronwyn O'Brien,Sheila DonnellyPubMed 34374810
- American journal of translational research 13:4632-46432021The protective role of miR-132 targeting HMGA2 through the PI3K/AKT pathway in mice with Alzheimer's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXichang Liu,Haitao Wang,Jiawei Bei,Jun Zhao,Ge Jiang,Xiuhong LiuPubMed 34150043
Key facts
- Host species
- Donkey
- Target species
- Goat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Hamster
- Conjugation
- Cy5®
- Applications
- WB, Flow Cyt, ICC, IHC-Fr, IHC-P, ICC/IF, ELISA
- Clonality
- Polyclonal
Abcam Recommends
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info - | Notes - |
Application ICC | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/10000.00000 - 1/50000.00000 | Notes - |
Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G
Recommended products
Suitable for WB, Flow Cyt, ICC, IHC-Fr, IHC-P, ICC/IF, ELISA. Preadsorbed to minimise non-specific binding and high background staining. Cited in 42 publications.
Key facts
- Description
- Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed
- Host species
- Donkey
- Target species
- Goat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Hamster
- Conjugation
- Cy5®
- Applications
- WB, Flow Cyt, ICC, IHC-Fr, IHC-P, ICC/IF, ELISA
- Clonality
- Polyclonal
- Pre-adsorbed
- Yes
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
pH: 6.5
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Tripotassium orthophosphate- Purification technique
- Affinity purification Immunogen
- Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Cy™ and CyDye™ are registered trademarks of Cytiva.
This secondaryantibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Li Q et al. ADAM17 is critical for multipolar exit and radial migration of neuronal intermediate progenitor cells in mice cerebral cortex. PLoS One 8:e65703 (2013).Immunohistochemistry (Frozen sections) - Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed (ab6566)
Embryonic brains were fixed in 4% paraformaldehyde in PBS overnight and placed in 15% sucrose/PBS for 12 h and then 30% sucrose/PBS for 24 h at 4°C. Brains were then embedded in OCT (optimum cutting temperature) mounting medium and froze before sectioning at 10 or 20 μm. Embryonic cerebral cortex from E14.5, E15.5, E16.5 and E18.5 mice were dissected out and sectioned at 10–15 μm for immunohistochemistry experiment with anti-ADAM17 (ab13535) antibody.
Cy5-labeled donkey anti-goat (ab6566) was used as the secondary antibody (Red).
This image is courtesy of an anonymous customer reviewWestern blot - Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed (ab6566)
All lanes: Goat anti-UCP1 at 1/200 dilution
Lane 1: Marker
Lanes 2 - 3: Mouse brown adipose tissue lysate at 70 µg
SecondaryAll lanes: Western blot - Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed (ab6566) at 1/2500 dilution
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