Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed

• IHC-Fr

PubMed

Immunohistochemistry (Frozen sections) - Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed (AB6566)

Embryonic brains were fixed in 4% paraformaldehyde in PBS overnight and placed in 15% sucrose/PBS for 12 h and then 30% sucrose/PBS for 24 h at 4°C. Brains were then embedded in OCT (optimum cutting temperature) mounting medium and froze before sectioning at 10 or 20 μm. Embryonic cerebral cortex from E14.5, E15.5, E16.5 and E18.5 mice were dissected out and sectioned at 10–15 μm for immunohistochemistry experiment with anti-ADAM17 (ab13535) antibody.

Cy5-labeled donkey anti-goat (ab6566) was used as the secondary antibody (Red).

Li Q et al. ADAM17 is critical for multipolar exit and radial migration of neuronal intermediate progenitor cells in mice cerebral cortex. PLoS One 8:e65703 (2013).

• WB

AbReview44086****

Western blot - Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed (AB6566)

All lanes:

Goat anti-UCP1 at 1/200 dilution

Lane 1:

Marker

Lanes 2 - 3:

Mouse brown adipose tissue lysate at 70 µg

Secondary

All lanes:

Western blot - Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed (ab6566) at 1/2500 dilution

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This image is courtesy of an anonymous Abreview