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Suitable for WB, Flow Cyt, ICC, IHC-Fr, IHC-P, ICC/IF, ELISA. Preadsorbed to minimise non-specific binding and high background staining. Cited in 43 publications.

Host species

Donkey

Target species

Goat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Hamster

Conjugation

Cy5®

Applications

WB, Flow Cyt, ICC, IHC-Fr, IHC-P, ICC/IF, ELISA

Clonality

Polyclonal

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Reactivity data

Application
WB
Reactivity
Reacts
Dilution info
-
Notes

-

Application
Flow Cyt
Reactivity
Reacts
Dilution info
-
Notes

-

Application
ICC
Reactivity
Reacts
Dilution info
-
Notes

-

Application
IHC-Fr
Reactivity
Reacts
Dilution info
-
Notes

-

Application
IHC-P
Reactivity
Reacts
Dilution info
-
Notes

-

Application
ICC/IF
Reactivity
Reacts
Dilution info
1/1000.00000 - 1/5000.00000
Notes

-

Application
ELISA
Reactivity
Reacts
Dilution info
1/10000.00000 - 1/50000.00000
Notes

-

Images

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Suitable for WB, Flow Cyt, ICC, IHC-Fr, IHC-P, ICC/IF, ELISA. Preadsorbed to minimise non-specific binding and high background staining. Cited in 43 publications.

Key facts

Description

Donkey Anti-Goat IgG H&L (Cy5 ®) preadsorbed

Host species

Donkey

Target species

Goat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Hamster

Conjugation

Cy5®

Applications

WB, Flow Cyt, ICC, IHC-Fr, IHC-P, ICC/IF, ELISA

Clonality

Polyclonal

Pre-adsorbed

Yes

Isotype

IgG

Concentration
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Properties

Form

Liquid

Storage buffer

pH: 6.5
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Tripotassium orthophosphate

Purification technique

Affinity purification Immunogen

Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis.

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Notes

Cy™ and CyDye™ are registered trademarks of Cytiva.

This secondaryantibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Fluorochrome chart

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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