AB6951

Donkey Anti-Goat IgG H&L (Cy5.5 ®) preadsorbed

Name: Donkey Anti Goat (IgG) secondary antibody preadsorbed Cy5.5® (ab6951) | Abcam
Brand: Abcam Limited
SKU: AB6951

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(11 Publications)

Suitable for Flow Cyt, ICC, IHC-Fr, IHC-P, ELISA, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining. Cited in 11 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

Key facts

Host species

Donkey

Target species

Goat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Rabbit, Horse, Guinea pig, Hamster, Cow

Pre-adsorbed

Yes

Conjugation

Cy5.5®

Excitation/Emission

Ex: 678nm, Em: 703nm

Applications

Flow Cyt, ELISA, ICC/IF, IHC-P, IHC-Fr, ICC

applications

Clonality

Polyclonal

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/50000", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/5000", "notes":"" } } }

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Product details

Cy™ and CyDye™ are registered trademarks of Cytiva.

This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above.

Storage buffer

pH: 6.5 Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.87% Sodium chloride, 0.42% Tripotassium orthophosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Goat IgG

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 84:1003-1020.e10 PubMed38359824

2024

Specialized replication mechanisms maintain genome stability at human centromeres.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Scelfo,Annapaola Angrisani,Marco Grillo,Bethany M Barnes,Francesc Muyas,Carolin M Sauer,Chin Wei Brian Leung,Marie Dumont,Marine Grison,David Mazaud,Mickaël Garnier,Laetitia Guintini,Louisa Nelson,Fumiko Esashi,Isidro Cortés-Ciriano,Stephen S Taylor,Jérôme Déjardin,Therese Wilhelm,Daniele Fachinetti

The EMBO journal 42:e110844 PubMed37661798

2023

In vivo reduction of RAD51-mediated homologous recombination triggers aging but impairs oncogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriel Matos-Rodrigues,Vilma Barroca,Ali-Akbar Muhammad,Elodie Dardillac,Awatef Allouch,Stephane Koundrioukoff,Daniel Lewandowski,Emmanuelle Despras,Josée Guirouilh-Barbat,Lucien Frappart,Patricia Kannouche,Pauline Dupaigne,Eric Le Cam,Jean-Luc Perfettini,Paul-Henri Romeo,Michelle Debatisse,Maria Jasin,Gabriel Livera,Emmanuelle Martini,Bernard S Lopez

International journal of molecular sciences 24: PubMed37445805

2023

Firing of Replication Origins Is Disturbed by a CDK4/6 Inhibitor in a pRb-Independent Manner.

Applications

Unspecified application

Species

Unspecified reactive species

Su-Jung Kim,Chrystelle Maric,Lina-Marie Briu,Fabien Fauchereau,Giuseppe Baldacci,Michelle Debatisse,Stéphane Koundrioukoff,Jean-Charles Cadoret

The EMBO journal 40:e104543 PubMed34533226

2021

DNA polymerase zeta contributes to heterochromatin replication to prevent genome instability.

Applications

Unspecified application

Species

Unspecified reactive species

Barbara Ben Yamin,Sana Ahmed-Seghir,Junya Tomida,Emmanuelle Despras,Caroline Pouvelle,Andrey Yurchenko,Jordane Goulas,Raphael Corre,Quentin Delacour,Nathalie Droin,Philippe Dessen,Didier Goidin,Sabine S Lange,Sarita Bhetawal,Maria Teresa Mitjavila-Garcia,Giuseppe Baldacci,Sergey Nikolaev,Jean Charles Cadoret,Richard D Wood,Patricia L Kannouche

Life (Basel, Switzerland) 11: PubMed33804994

2021

The Telomeric Protein TRF2 Regulates Replication Origin Activity within Pericentromeric Heterochromatin.

Applications

Unspecified application

Species

Unspecified reactive species

Serge Bauwens,Liudmyla Lototska,Stephane Koundrioukoff,Michelle Debatisse,Jing Ye,Eric Gilson,Aaron Mendez-Bermudez

Molecular cell 70:449-461.e5 PubMed29727617

2018

Genome-wide Control of Heterochromatin Replication by the Telomere Capping Protein TRF2.

Applications

Unspecified application

Species

Unspecified reactive species

Aaron Mendez-Bermudez,Liudmyla Lototska,Serge Bauwens,Marie-Josèphe Giraud-Panis,Olivier Croce,Karine Jamet,Agurtzane Irizar,Macarena Mowinckel,Stephane Koundrioukoff,Nicolas Nottet,Genevieve Almouzni,Mare-Paule Teulade-Fichou,Michael Schertzer,Mylène Perderiset,Arturo Londoño-Vallejo,Michelle Debatisse,Eric Gilson,Jing Ye

Journal of neuroinflammation 15:45 PubMed29452577

2018

Polymerase delta-interacting protein 2 deficiency protects against blood-brain barrier permeability in the ischemic brain.

Applications

Unspecified application

Species

Unspecified reactive species

Marina S Hernandes,Bernard Lassègue,Lula L Hilenski,Jonathan Adams,Ning Gao,Chia-Yi Kuan,Yu-Yo Sun,Lihong Cheng,Daniel S Kikuchi,Manuel Yepes,Kathy K Griendling

Cell reports 7:575-587 PubMed24726359

2014

The RBBP6/ZBTB38/MCM10 axis regulates DNA replication and common fragile site stability.

Applications

ICC/IF

Species

Unspecified reactive species

Benoit Miotto,Moredreck Chibi,Ping Xie,Stéphane Koundrioukoff,Hanlie Moolman-Smook,David Pugh,Michelle Debatisse,Fuchu He,Lingqiang Zhang,Pierre-Antoine Defossez

Proceedings of the National Academy of Sciences of the United States of America 111:763-8 PubMed24347643

2013

Spontaneous slow replication fork progression elicits mitosis alterations in homologous recombination-deficient mammalian cells.

Applications

Unspecified application

Species

Unspecified reactive species

Therese Wilhelm,Indiana Magdalou,Aurélia Barascu,Hervé Técher,Michelle Debatisse,Bernard S Lopez

Journal of molecular biology 425:4845-55 PubMed23557832

2013

Replication dynamics: biases and robustness of DNA fiber analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Hervé Técher,Stéphane Koundrioukoff,Dana Azar,Therese Wilhelm,Sandra Carignon,Olivier Brison,Michelle Debatisse,Benoît Le Tallec

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey Anti-Goat IgG H&L (Cy5.5 ®) preadsorbed

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (11)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise