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Suitable for ICC/IF, Flow Cyt, IHC-P, WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 7 publications.

Host species

Donkey

Target species

Goat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Rabbit, Horse, Chicken, Human, Pig

Conjugation

DyLight® 488

Applications

ICC/IF, Flow Cyt, IHC-P, WB

Clonality

Polyclonal

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Reactivity data

Application
ICC/IF
Reactivity
Reacts
Dilution info
1/50.00000 - 1/500.00000
Notes

-

Application
Flow Cyt
Reactivity
Reacts
Dilution info
1/50.00000 - 1/200.00000
Notes

-

Application
IHC-P
Reactivity
Reacts
Dilution info
1/50.00000 - 1/500.00000
Notes

-

Application
WB
Reactivity
Reacts
Dilution info
1/1000.00000 - 1/2000.00000
Notes
5% non-fat dry milk in PBST or TBST is recommended for blocking and incubation of antibodies. BSA is not recommended.
Target data

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Suitable for ICC/IF, Flow Cyt, IHC-P, WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 7 publications.

Key facts

Description

Donkey Anti-Goat IgG H&L (DyLight® 488) preadsorbed

Host species

Donkey

Target species

Goat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Rabbit, Horse, Chicken, Human, Pig

Conjugation

DyLight® 488

Applications

ICC/IF, Flow Cyt, IHC-P, WB

Clonality

Polyclonal

Pre-adsorbed

Yes

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to chicken, horse, human, mouse, pig, rabbit and rat was detected.

Isotype

IgG

Concentration
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Properties

Form

Liquid

Storage buffer

pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using chicken, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Fluorochrome chart

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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