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Suitable for ICC/IF, Flow Cyt, IHC-P, WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 8 publications.


Publications

Key facts

Host species
Donkey
Target species
Goat
Target isotype
IgG
Target specificity
Heavy & Light chains
Minimal cross-reactivity
Mouse, Rat, Rabbit, Horse, Chicken, Human, Pig
Conjugation
DyLight® 488
Applications
ICC/IF, Flow Cyt, IHC-P, WB
Clonality
Polyclonal

Reactivity data

Application
ICC/IF
Reactivity
Reacts
Dilution info
1/50.00000 - 1/500.00000
Notes

-

Application
Flow Cyt
Reactivity
Reacts
Dilution info
1/50.00000 - 1/200.00000
Notes

-

Application
IHC-P
Reactivity
Reacts
Dilution info
1/50.00000 - 1/500.00000
Notes

-

Application
WB
Reactivity
Reacts
Dilution info
1/1000.00000 - 1/2000.00000
Notes

5% non-fat dry milk in PBST or TBST is recommended for blocking and incubation of antibodies. BSA is not recommended.

Target data

Alternative names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

Recommended products

Suitable for ICC/IF, Flow Cyt, IHC-P, WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 8 publications.

Key facts

Description
Donkey Anti-Goat IgG H&L (DyLight® 488) preadsorbed
Host species
Donkey
Target species
Goat
Target isotype
IgG
Target specificity
Heavy & Light chains
Minimal cross-reactivity
Mouse, Rat, Rabbit, Horse, Chicken, Human, Pig
Conjugation
DyLight® 488
Applications
ICC/IF, Flow Cyt, IHC-P, WB
Clonality
Polyclonal
Pre-adsorbed
Yes
Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to chicken, horse, human, mouse, pig, rabbit and rat was detected.

Isotype
IgG
Concentration
Loading...

Properties

Form
Liquid
Storage buffer

pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA

Purification technique
Affinity purification Immunogen
Purification notes

Antiserum was cross adsorbed using chicken, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

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