AB96935

Donkey Anti-Goat IgG H&L (DyLight® 488) preadsorbed

Name: Donkey Anti Goat (IgG) secondary antibody preadsorbed DyLight® 488 (ab96935)
Brand: Abcam Limited
SKU: AB96935
Rating: 5 (1 reviews)

(1 Review)

(9 Publications)

Suitable for ICC/IF, Flow Cyt, IHC-P, WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 9 publications.

Key facts

Host species

Donkey

Target species

Goat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Rabbit, Horse, Chicken, Human, Pig

Pre-adsorbed

Yes

Conjugation

DyLight® 488

Excitation/Emission

Ex: 493nm, Em: 518nm

Applications

WB, IHC-P, Flow Cyt, ICC/IF

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to chicken, horse, human, mouse, pig, rabbit and rat was detected.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/200", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/2000", "notes":"5% non-fat dry milk in PBST or TBST is recommended for blocking and incubation of antibodies. BSA is not recommended." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using chicken, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Goat IgG

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 9: PubMed38888971

2024

A dual-acting DNASE1/DNASE1L3 biologic prevents autoimmunity and death in genetic and induced lupus models.

Applications

Unspecified application

Species

Unspecified reactive species

Paul R Stabach,Dominique Sims,Eduardo Gomez-Bañuelos,Sandra Zehentmeier,Kris Dammen-Brower,Andrew Bernhisel,Sophia Kujawski,Sam G Lopez,Michelle Petri,Daniel W Goldman,Ethan R Lester,Quan Le,Tayyaba Ishaq,Hana Kim,Shivani Srivastava,Deepika Kumar,Joao P Pereira,Kevin J Yarema,Fotios Koumpouras,Felipe Andrade,Demetrios T Braddock

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2303128 PubMed38348560

2024

Combinatorial Microgels for 3D ECM Screening and Heterogeneous Microenvironmental Culture of Primary Human Hepatic Stellate Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hyeon Ryoo,Regina Giovanni,Hannah Kimmel,Ishita Jain,Gregory H Underhill

Advanced healthcare materials 10:e2100223 PubMed33890430

2021

Microtissue Geometry and Cell-Generated Forces Drive Patterning of Liver Progenitor Cell Differentiation in 3D.

Applications

Unspecified application

Species

Unspecified reactive species

Ian C Berg,Erfan Mohagheghian,Krista Habing,Ning Wang,Gregory H Underhill

Frontiers in bioengineering and biotechnology 8:792 PubMed32793571

2020

Integration of Hydrogel Microparticles With Three-Dimensional Liver Progenitor Cell Spheroids.

Applications

Unspecified application

Species

Unspecified reactive species

Stefan D Gentile,Andreas P Kourouklis,Hyeon Ryoo,Gregory H Underhill

eLife 7: PubMed30589410

2018

Spatial patterning of liver progenitor cell differentiation mediated by cellular contractility and Notch signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Kerim B Kaylan,Ian C Berg,Matthew J Biehl,Aidan Brougham-Cook,Ishita Jain,Sameed M Jamil,Lauren H Sargeant,Nicholas J Cornell,Lori T Raetzman,Gregory H Underhill

Nature communications 9:1597 PubMed29686427

2018

A mobile endocytic network connects clathrin-independent receptor endocytosis to recycling and promotes T cell activation.

Applications

Unspecified application

Species

Unspecified reactive species

Ewoud B Compeer,Felix Kraus,Manuela Ecker,Gregory Redpath,Mayan Amiezer,Nils Rother,Philip R Nicovich,Natasha Kapoor-Kaushik,Qiji Deng,Guerric P B Samson,Zhengmin Yang,Jieqiong Lou,Michael Carnell,Haig Vartoukian,Katharina Gaus,Jérémie Rossy

Cardiovascular diabetology 16:142 PubMed29096622

2017

Tissue-engineered smooth muscle cell and endothelial progenitor cell bi-level cell sheets prevent progression of cardiac dysfunction, microvascular dysfunction, and interstitial fibrosis in a rodent model of type 1 diabetes-induced cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Masashi Kawamura,Michael J Paulsen,Andrew B Goldstone,Yasuhiro Shudo,Hanjay Wang,Amanda N Steele,Lyndsay M Stapleton,Bryan B Edwards,Anahita Eskandari,Vi N Truong,Kevin J Jaatinen,Arnar B Ingason,Shigeru Miyagawa,Yoshiki Sawa,Y Joseph Woo

Developmental biology 432:98-124 PubMed28882402

2017

Visceral endoderm and the primitive streak interact to build the fetal-placental interface of the mouse gastrula.

Applications

Unspecified application

Species

Unspecified reactive species

Adriana M Rodriguez,Karen M Downs

Scientific reports 6:23490 PubMed27025873

2016

Combinatorial microenvironmental regulation of liver progenitor differentiation by Notch ligands, TGFβ, and extracellular matrix.

Applications

Unspecified application

Species

Unspecified reactive species

Kerim B Kaylan,Viktoriya Ermilova,Ravi Chandra Yada,Gregory H Underhill

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey Anti-Goat IgG H&L (DyLight® 488) preadsorbed

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Complementary Products

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (9)

Product promise