AB96932

Donkey Anti-Goat IgG H&L (DyLight® 550)

Name: Donkey Anti Goat (IgG) secondary antibody DyLight® 550 (ab96932) | Abcam
Brand: Abcam Limited
SKU: AB96932
Price: 265 USD
Availability: InStock

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(7 Publications)

Suitable for IHC-P, Flow Cyt, ICC/IF. Cited in 7 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

Key facts

Host species

Donkey

Target species

Goat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

DyLight® 550

Excitation/Emission

Ex: 562nm, Em: 576nm

Applications

Flow Cyt, ICC/IF, IHC-P

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550. This information however will have no impact on your experiments." }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/200", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"" } } }

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Product details

DyLight® 550 replaces DyLight® 549.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Goat IgG

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Nutrients 15: PubMed36904077

2023

Stimulation of GHRH Neuron Axon Growth by Leptin and Impact of Nutrition during Suckling in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Lyvianne Decourtye-Espiard,Maud Clemessy,Patricia Leneuve,Erik Mire,Tatiana Ledent,Yves Le Bouc,Laurent Kappeler

Cells 12: PubMed36831212

2023

An Optimized Workflow to Generate and Characterize iPSC-Derived Motor Neuron (MN) Spheroids.

Applications

Unspecified application

Species

Unspecified reactive species

María José Castellanos-Montiel,Mathilde Chaineau,Anna Kristyna Franco-Flores,Ghazal Haghi,Dulce Carrillo-Valenzuela,Wolfgang E Reintsch,Carol X-Q Chen,Thomas M Durcan

Frontiers in neurology 11:983 PubMed33013649

2020

20-HETE Enzymes and Receptors in the Neurovascular Unit: Implications in Cerebrovascular Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Ezekiel Gonzalez-Fernandez,Daniel Staursky,Kathryn Lucas,Bond V Nguyen,Man Li,Yedan Liu,Chad Washington,Lique M Coolen,Fan Fan,Richard J Roman

Developmental biology 425:208-222 PubMed28389228

2017

Brachyury drives formation of a distinct vascular branchpoint critical for fetal-placental arterial union in the mouse gastrula.

Applications

Unspecified application

Species

Unspecified reactive species

Adriana M Rodriguez,Dexter X Jin,Adam D Wolfe,Maria M Mikedis,Lauren Wierenga,Maleka P Hashmi,Christoph Viebahn,Karen M Downs

International journal of molecular medicine 39:569-578 PubMed28204828

2017

TIEG1 deficiency confers enhanced myocardial protection in the infarcted heart by mediating the Pten/Akt signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Mingqiu Cen,Pengfei Hu,Zhaobin Cai,Tianfu Fang,Jiancheng Zhang,Ming Lu

Developmental dynamics : an official publication of the American Association of Anatomists 246:50-71 PubMed27696611

2016

PRDM1/BLIMP1 is widely distributed to the nascent fetal-placental interface in the mouse gastrula.

Applications

Unspecified application

Species

Unspecified reactive species

Maria M Mikedis,Karen M Downs

Gene expression patterns : GEP 13:225-39 PubMed23639725

2013

Widespread but tissue-specific patterns of interferon-induced transmembrane protein 3 (IFITM3, FRAGILIS, MIL-1) in the mouse gastrula.

Applications

Unspecified application

Species

Unspecified reactive species

Maria M Mikedis,Karen M Downs

View all publications

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Product promise

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Donkey Anti-Goat IgG H&L (DyLight® 550)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (7)

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