AB96936

Donkey Anti-Goat IgG H&L (DyLight® 550) preadsorbed

Name: Donkey Anti Goat (IgG) secondary antibody preadsorbed DyLight® 550 (ab96936)
Brand: Abcam Limited
SKU: AB96936
Price: 295 USD
Availability: InStock

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(4 Publications)

Suitable for IHC-P, Flow Cyt, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining. Cited in 4 publications.

Key facts

Host species

Donkey

Target species

Goat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Rabbit, Horse, Chicken, Human, Pig

Pre-adsorbed

Yes

Conjugation

DyLight® 550

Excitation/Emission

Ex: 562nm, Em: 576nm

Applications

IHC-P, ICC/IF, Flow Cyt

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to chicken, horse, human, mouse, pig, rabbit and rat was detected.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550. This information however will have no impact on your experiments." }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/200", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"" } } }

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Product details

DyLight® 550 replaces DyLight® 549.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using chicken, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Goat IgG

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Development, growth & differentiation 66:106-118 PubMed38197329

2024

Transcription factor Tcf21 modulates urinary bladder size and differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth A Mann,Melissa S Mogle,Joo-Seop Park,Pramod Reddy

PloS one 15:e0235543 PubMed32645028

2020

Correlation of pyroglutamate amyloid β and ptau Ser202/Thr205 levels in Alzheimer's disease and related murine models.

Applications

Unspecified application

Species

Unspecified reactive species

Joerg Neddens,Magdalena Daurer,Stefanie Flunkert,Kerstin Beutl,Tina Loeffler,Lauren Walker,Johannes Attems,Birgit Hutter-Paier

EBioMedicine 46:387-398 PubMed31327694

2019

Human bone marrow adipocytes display distinct immune regulatory properties.

Applications

Unspecified application

Species

Unspecified reactive species

Carina Miggitsch,Andreas Meryk,Erin Naismith,Luca Pangrazzi,Asim Ejaz,Brigitte Jenewein,Sonja Wagner,Fabiana Nägele,Gabriella Fenkart,Klemens Trieb,Werner Zwerschke,Beatrix Grubeck-Loebenstein

Cell reports 3:427-41 PubMed23403291

2013

c-MYC-induced sebaceous gland differentiation is controlled by an androgen receptor/p53 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Denny L Cottle,Kai Kretzschmar,Pawel J Schweiger,Sven R Quist,Harald P Gollnick,Ken Natsuga,Satoru Aoyagi,Fiona M Watt

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey Anti-Goat IgG H&L (DyLight® 550) preadsorbed

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (4)

Product promise