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Suitable for ICC/IF, Flow Cyt, IHC-P. Preadsorbed to minimise non-specific binding and high background staining. Cited in 9 publications.


Publications

Key facts

Host species
Donkey
Target species
Goat
Target isotype
IgG
Target specificity
Heavy & Light chains
Minimal cross-reactivity
Mouse, Rat, Rabbit, Horse, Chicken, Human, Pig
Conjugation
DyLight® 650
Applications
ICC/IF, Flow Cyt, IHC-P
Clonality
Polyclonal

Reactivity data

Application
ICC/IF
Reactivity
Reacts
Dilution info
1/50.00000 - 1/500.00000
Notes

-

Application
Flow Cyt
Reactivity
Reacts
Dilution info
1/50.00000 - 1/200.00000
Notes

-

Application
IHC-P
Reactivity
Reacts
Dilution info
1/50.00000 - 1/500.00000
Notes

DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments.

Alternative names

Recommended products

Suitable for ICC/IF, Flow Cyt, IHC-P. Preadsorbed to minimise non-specific binding and high background staining. Cited in 9 publications.

Key facts

Description
Donkey Anti-Goat IgG H&L (DyLight® 650) preadsorbed
Host species
Donkey
Target species
Goat
Target isotype
IgG
Target specificity
Heavy & Light chains
Minimal cross-reactivity
Mouse, Rat, Rabbit, Horse, Chicken, Human, Pig
Conjugation
DyLight® 650
Applications
ICC/IF, Flow Cyt, IHC-P
Clonality
Polyclonal
Pre-adsorbed
Yes
Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to chicken, horse, human, mouse, pig, rabbit and rat was detected.

Isotype
IgG
Concentration
Loading...

Properties

Form
Liquid
Storage buffer

pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA

Purification technique
Affinity purification Immunogen
Purification notes

Antiserum was cross adsorbed using chicken, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

DyLight® 650 replaces DyLight® 649.

Product promise

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