AB96938

Donkey Anti-Goat IgG H&L (DyLight® 650) preadsorbed

Name: Donkey Anti-Goat IgG (DyLight® 650) preadsorbed secondary antibody (ab96938) | Abcam
Brand: Abcam Limited
SKU: AB96938
Rating: 5 (1 reviews)

(1 Review)

(11 Publications)

Suitable for ICC/IF, Flow Cyt, IHC-P. Preadsorbed to minimise non-specific binding and high background staining. Cited in 11 publications.

Key facts

Host species

Donkey

Target species

Goat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Rabbit, Horse, Chicken, Human, Pig

Pre-adsorbed

Yes

Conjugation

DyLight® 650

Excitation/Emission

Ex: 654nm, Em: 673nm

Applications

Flow Cyt, ICC/IF, IHC-P

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to chicken, horse, human, mouse, pig, rabbit and rat was detected.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/200", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments." } } }

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Product details

DyLight® 650 replaces DyLight® 649.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using chicken, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Goat IgG

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Brain structure & function 230:30 PubMed39805973

2025

Basal forebrain innervation of the amygdala: an anatomical and computational exploration.

Applications

Unspecified application

Species

Unspecified reactive species

Tuğçe Tuna,Tyler Banks,Gregory Glickert,Cem Sevinc,Satish S Nair,Gunes Unal

Progress in neuro-psychopharmacology & biological psychiatry 125:110760 PubMed37031946

2023

Differential role of GABAergic and cholinergic ventral pallidal neurons in behavioral despair, conditioned fear memory and active coping.

Applications

Unspecified application

Species

Unspecified reactive species

Cemal Akmese,Cem Sevinc,Sahar Halim,Gunes Unal

Experimental neurology 360:114278 PubMed36455639

2022

Delayed viral vector mediated delivery of neurotrophin-3 improves skilled hindlimb function and stability after thoracic contusion.

Applications

Unspecified application

Species

Unspecified reactive species

Jared D Sydney-Smith,Alice M Koltchev,Lawrence D F Moon,Philippa M Warren

Nature communications 11:5031 PubMed33024112

2020

Mechanism and evolution of the Zn-fingernail required for interaction of VARP with VPS29.

Applications

Unspecified application

Species

Unspecified reactive species

Harriet Crawley-Snowdon,Ji-Chun Yang,Nathan R Zaccai,Luther J Davis,Lena Wartosch,Emily K Herman,Nicholas A Bright,James S Swarbrick,Brett M Collins,Lauren P Jackson,Matthew N J Seaman,J Paul Luzio,Joel B Dacks,David Neuhaus,David J Owen

Data in brief 21:377-385 PubMed30364576

2018

RNA sequencing dataset describing transcriptional changes in cervical dorsal root ganglia after bilateral pyramidotomy and forelimb intramuscular gene therapy with an adeno-associated viral vector encoding human neurotrophin-3.

Applications

Unspecified application

Species

Unspecified reactive species

Claudia Kathe,Lawrence D F Moon

PloS one 13:e0197674 PubMed29787578

2018

mTh1 driven expression of hTDP-43 results in typical ALS/FTLD neuropathological symptoms.

Applications

Unspecified application

Species

Unspecified reactive species

Barbara Scherz,Roland Rabl,Stefanie Flunkert,Siegfried Rohler,Joerg Neddens,Nicole Taub,Magdalena Temmel,Ute Panzenboeck,Vera Niederkofler,Robert Zimmermann,Birgit Hutter-Paier

Developmental biology 432:98-124 PubMed28882402

2017

Visceral endoderm and the primitive streak interact to build the fetal-placental interface of the mouse gastrula.

Applications

Unspecified application

Species

Unspecified reactive species

Adriana M Rodriguez,Karen M Downs

American journal of physiology. Renal physiology 313:F85-F102 PubMed28331065

2017

Urothelial proliferation and regeneration after spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

F Aura Kullmann,Dennis R Clayton,Wily G Ruiz,Amanda Wolf-Johnston,Christian Gauthier,Anthony Kanai,Lori A Birder,Gerard Apodaca

The Journal of experimental medicine 213:2885-2896 PubMed27821549

2016

Mycolactone subverts immunity by selectively blocking the Sec61 translocon.

Applications

Unspecified application

Species

Unspecified reactive species

Ludivine Baron,Anja Onerva Paatero,Jean-David Morel,Francis Impens,Laure Guenin-Macé,Sarah Saint-Auret,Nicolas Blanchard,Rabea Dillmann,Fatoumata Niang,Sandra Pellegrini,Jack Taunton,Ville O Paavilainen,Caroline Demangel

eLife 5: PubMed27759565

2016

Intramuscular Neurotrophin-3 normalizes low threshold spinal reflexes, reduces spasms and improves mobility after bilateral corticospinal tract injury in rats.

Applications

Unspecified application

Species

Rat

Claudia Kathe,Thomas Haynes Hutson,Stephen Brendan McMahon,Lawrence David Falcon Moon

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey Anti-Goat IgG H&L (DyLight® 650) preadsorbed

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (11)

Product promise