Donkey Anti-Goat IgG H&L (HRP)

• IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (HRP) (AB6885)

Immunohistologic images of expression of endometrial PECAM-1/CD31 in (Panel A) controls and (Panel B) IRSM (Idiopathic recurrent spontaneous miscarriage).

3-5 μm thick sections obtained from formaldehyde fixed, paraffin-embedded human tissue were dehydrated in graded ethanol. After antigen retrieval, slides were incubated for an hour in 3% blocking serum (BSA) in PBS for controlling non-specific binding of primary antibody. The slides were then incubated with goat anti-PECAM 1. Excess primary antibody was washed with PBS and the sections were again incubated with ab6885.

Labeled cells were visualized with diaminobenzidine (DAB) and sections counterstained with hematoxylin. Next, the slides were dehydrated using series of alcohol gradient and mounted using distrene, tricresyl phosphate (DPX) and xylene.

Banerjee et al PLoS One. 2013 Nov 15;8(11):e80940. doi: 10.1371/journal.pone.0080940. eCollection 2013. Fig 2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (HRP) (AB6885)

IHC image of beta Actin staining in human normal colon formalin fixed paraffin embedded tissue section*.

The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6) for 30 mins. The section was incubated with ab8229,10 μg/ml overnight at +4°C. An HRP-conjugated secondary (ab6885, 1/250 dilution) was used for 1 hr at room temperature. The section was counterstained with hematoxylin and mounted with DPX.

The inset negative control image is taken from an identical assay without primary antibody.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• ELISA

Supplier Data

ELISA - Donkey Anti-Goat IgG H&L (HRP) (AB6885)

ELISA results of ab6885 against purified Goat IgG. Each well was coated in duplicate with 1.0 µg of Goat IgG. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer, and TMB substrate.

• WB

Unknown

Western blot - Donkey Anti-Goat IgG H&L (HRP) (AB6885)

ab6885 was used at dilution 1/5000 with the primary antibody ab36146 in WB. See the review on ab36146.

Secondary

All lanes:

Western blot - Donkey Anti-Goat IgG H&L (HRP) (ab6885) at 1/5000 dilution

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• WB

PubMed

Western blot - Donkey Anti-Goat IgG H&L (HRP) (AB6885)

Expression levels of wild type and variant GFP-CDH13 fusion proteins in HEK-293 cells.

Panel A : GFP-CDH13 fusion proteins (26 kDa+105 kDa = 131 kDa) were detected in HEK-293 cells by an antibody against GFP. Mock cells transfected with the empty GFP vector expressed only GFP (26 kDa).

ab6885 used at a 1 : 5000 dilution.

Secondary

All lanes:

Western blot - Donkey Anti-Goat IgG H&L (HRP) (ab6885)

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Mavroconstanti et al PLoS One. 2013 Aug 1;8(8):e71445. doi: 10.1371/journal.pone.0071445. Print 2013. Fig S1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/