Donkey Anti-Goat IgG H&L (HRP)

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (HRP) (AB97110)

IHC image of beta actin staining in human normal colon formalin fixed paraffin embedded tissue section*.

The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6) for 30 mins. The section was incubated with ab8229, 10 μg/ml overnight at +4°C. An HRP-conjugated secondary (ab97110, 1/100 dilution) was used for 1 hour at room temperature. The section was counterstained with hematoxylin and mounted with DPX.

The inset negative control image is secondary-only at 1/200 dilution.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• WB

Unknown

Western blot - Donkey Anti-Goat IgG H&L (HRP) (AB97110)

All lanes:

Western blot - Anti-beta Actin antibody - Loading Control (<a href='/en-us/products/primary-antibodies/beta-actin-antibody-loading-control-ab8229'>ab8229</a>) at 1 µg/mL

All lanes:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Secondary

Lanes 1, 2, 5 and 6:

Western blot - Donkey Anti-Goat IgG H&L (HRP) (ab97110) at 1/20000 dilution

Lanes 3 - 4:

Western blot - Donkey Anti-Goat IgG H&L (HRP) (ab97110) at 1/10000 dilution

Predicted band size: 36 kDa

true

Exposure time: 20min