Donkey anti-goat IgG H&L (HRP) is a secondary antibody for chemiluminescent detection. Ideal for western blot. Suitable for ICC/IF, IHC, flow cytometry and ELISA.
- Cited in over 90 publications
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- BMC pharmacology & toxicology 24:582023GHB toxicokinetics and renal monocarboxylate transporter expression are influenced by the estrus cycle in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHao Wei,Jieyun Cao,Tyler Fallert,Su Yeo,Melanie A FelmleePubMed 37919807
- Frontiers in endocrinology 14:12682482023Expression of genes and enzymes involved in ovarian steroidogenesis in relation to human follicular development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengxue Zheng,Claus Yding Andersen,Frida Roikjer Rasmussen,Jesús Cadenas,Søren Tvorup Christensen,Linn Salto MamsenPubMed 37964966
- Biomedicines 11:2023Genetic and Pharmacological Blockade of Sigma-1 Receptors Attenuates Inflammation-Associated Hypersensitivity during Acute Colitis in CD1 Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSergio López-Estévez,Mònica Aguilera,Georgia Gris,Beatriz de la Puente,Alicia Carceller,Vicente MartínezPubMed 37893131
- Nature communications 14:53282023Nacα protects the larval fat body from cell death by maintaining cellular proteostasis in Drosophila.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTakayuki Yamada,Yuto Yoshinari,Masayuki Tobo,Okiko Habara,Takashi NishimuraPubMed 37658058
- Journal of cell science 136:2023Coupled protein quality control during nonsense-mediated mRNA decay.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlison J Inglis,Alina Guna,Ángel Gálvez-Merchán,Akshaye Pal,Theodore K Esantsi,Heather R Keys,Evgeni M Frenkel,Robert Oania,Jonathan S Weissman,Rebecca M VoorheesPubMed 37218462
- Stem cells translational medicine 12:281-2922023Correction of T-Cell Repertoire and Autoimmune Diabetes in NOD Mice by Non-myeloablative T-Cell Depleted Allogeneic HSCT.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRakefet Sidlik Muskatel,Bar Nathansohn-Levi,Shlomit Reich-Zeliger,Michal Mark,Liat Stoler-Barak,Chava Rosen,Irit Milman-Krentsis,Esther Bachar Lustig,Robert Pete Gale,Nir Friedman,Yair ReisnerPubMed 37184893
- Cancers 15:2023ImmunoPET Imaging Identifies the Optimal Timepoint for Combination Therapy in Xenograft Models of Triple-Negative Breast Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZiqi Li,Erika Belitzky,Ondrej Blaha,Alessandra Cavaliere,Samantha R Katz,Mariam Aboian,Lindy Melegari,Khashayar Rajabimoghadam,Stephen Kurpiewski,Xiaohua Zhu,Bernadette Marquez-NostraPubMed 36900378
- Antiviral research 212:1055582023CDK4/6 inhibitor palbociclib promotes SARS-CoV-2 cell entry by down-regulating SKP2 dependent ACE2 degradation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYingzi Xiao,Ying Yan,Le Chang,Huimin Ji,Huizhen Sun,Shi Song,Kaihao Feng,Abudulimutailipu Nuermaimaiti,Zhuoqun Lu,Lunan WangPubMed 36806814
- Nature communications 14:4642023A modular vaccine platform enabled by decoration of bacterial outer membrane vesicles with biotinylated antigens.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKevin B Weyant,Ayomide Oloyede,Sukumar Pal,Julie Liao,Mariela Rivera-De Jesus,Thapakorn Jaroentomeechai,Tyler D Moeller,Steven Hoang-Phou,Sean F Gilmore,Riya Singh,Daniel C Pan,David Putnam,Christopher Locher,Luis M de la Maza,Matthew A Coleman,Matthew P DeLisaPubMed 36709333
- Cancer research 83:814-8292023KDM6A Loss Triggers an Epigenetic Switch That Disrupts Urothelial Differentiation and Drives Cell Proliferation in Bladder Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHong Qiu,Vladimir Makarov,Jennifer K Bolzenius,Angela Halstead,Yvonne Parker,Allen Wang,Gopakumar V Iyer,Hannah Wise,Daniel Kim,Varna Thayaparan,Daniel J Lindner,Georges-Pascal Haber,Angela H Ting,Bing Ren,Timothy A Chan,Vivek Arora,David B Solit,Byron H LeePubMed 36638328
Key facts
- Host species
- Donkey
- Target species
- Goat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- HRP
- Applications
- IHC-P, ELISA, ICC, WB
- Clonality
- Polyclonal
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application IHC-P | Reactivity Reacts | Dilution info 1/200.00000 - 1/500.00000 | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/10000.00000 - 1/100000.00000 | Notes (Primary). |
Application ICC | Reactivity Reacts | Dilution info 1/200.00000 - 1/500.00000 | Notes - |
Application WB | Reactivity Reacts | Dilution info 1/1000.00000 - 1/30000.00000 | Notes Colorimetric: 1/1000 - 1/10000. Chemiluminescent: 1/1000 - 1/30000. In some cases the antibody may be diluted further than indicated. |
Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G
Recommended products
Donkey anti-goat IgG H&L (HRP) is a secondary antibody for chemiluminescent detection. Ideal for western blot. Suitable for ICC/IF, IHC, flow cytometry and ELISA.
- Cited in over 90 publications
Key facts
- Description
- Donkey Anti-Goat IgG H&L (HRP)
- Host species
- Donkey
- Target species
- Goat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- HRP
- Applications
- IHC-P, ELISA, ICC, WB
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.05% CMIT/MIT based preservative- Purification technique
- Affinity purification Immunogen
- Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies , designed to deliver precise and reliable results.
Part of the AbExcel range.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Goat IgG H&L (HRP) (ab97110)
IHC image of beta actin staining in human normal colon formalin fixed paraffin embedded tissue section*.
The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6) for 30 mins. The section was incubated with Anti-beta Actin antibody - Loading Control ab8229, 10 μg/ml overnight at +4°C. An HRP-conjugated secondary (ab97110, 1/100 dilution) was used for 1 hour at room temperature. The section was counterstained with hematoxylin and mounted with DPX.
The inset negative control image is secondary-only at 1/200 dilution.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Western blot - Donkey Anti-Goat IgG H&L (HRP) (ab97110)
All lanes: Western blot - Anti-beta Actin antibody - Loading Control (Anti-beta Actin antibody - Loading Control ab8229) at 1 µg/mL
All lanes: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
SecondaryLanes 1, 2, 5 and 6: Western blot - Donkey Anti-Goat IgG H&L (HRP) (ab97110) at 1/20000 dilution
Lanes 3 - 4: Western blot - Donkey Anti-Goat IgG H&L (HRP) (ab97110) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Exposure time: 20min
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