AB7125

Donkey Anti-Goat IgG H&L (HRP) preadsorbed

Name: Donkey Anti Goat (IgG) secondary antibody preadsorbed HRP (ab7125) | Abcam
Brand: Abcam Limited
SKU: AB7125
Price: 295 USD
Availability: InStock

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(15 Publications)

Suitable for Dot, IHC-Fr, IHC-P, IM, ICC, WB, ELISA. Ideal for western blot. Preadsorbed to minimise non-specific binding and high background staining. Cited in 15 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

3 Images

Supplier Data

Western blot - Donkey Anti-Goat IgG H&L (HRP) preadsorbed (AB7125)

* "Doublecortin microtubule affinity is regulated by a balance of kinase and phosphatase activity at the leading edge of migrating neurons." Schaar B.T., Kinoshita K., McConnell S.K. Neuron 41 : 203213 (2004).

Minimal cortex defined : small portion of cortex removed prior to lysis.

All lanes:

Western blot - Anti-Doublecortin antibody (<a href='/en-us/products/primary-antibodies/doublecortin-antibody-ab235153'>ab235153</a>) at 1/1000 dilution

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 15 µg

Lane 2:

Post-natal rat pup whole brain lysate (PND2-6)* at 15 µg

Lane 3:

Rat pup PND2-6 - minimal cortex brain lysate at 15 µg

Lane 4:

Rat pup PND2-6 – minimal cortex brain lysate at 35 µg

Secondary

All lanes:

Western blot - Donkey Anti-Goat IgG H&L (HRP) preadsorbed (ab7125) at 1/40000 dilution

Predicted band size: 49 kDa

Observed band size: 45 kDa

false

Exposure time: 15s

Unknown

Western blot - Donkey Anti-Goat IgG H&L (HRP) preadsorbed (AB7125)

ab7125 was used at dilution 1/10000 with the primary antibody ab37917 in WB. See the review on ab37917.

Secondary

All lanes:

Western blot - Donkey Anti-Goat IgG H&L (HRP) preadsorbed (ab7125)

false

Supplier Data

Western blot - Donkey Anti-Goat IgG H&L (HRP) preadsorbed (AB7125)

All lanes:

Western blot - Anti-Doublecortin antibody (<a href='/en-us/products/primary-antibodies/doublecortin-antibody-ab235153'>ab235153</a>) at 1/1000 dilution

Lane 1:

Doublecortin (human) overexpressing HEK-293T lysate C-Myc/DDK tagged

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Lane 3:

Adult mouse normal brain tissue lysate

Lane 4:

Adult rat normal brain tissue lysate

Secondary

All lanes:

Western blot - Donkey Anti-Goat IgG H&L (HRP) preadsorbed (ab7125) at 1/40000 dilution

Predicted band size: 49 kDa

Observed band size: 35 kDa,48 kDa

false

Exposure time: 15s

Key facts

Host species

Donkey

Target species

Goat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Hamster

Pre-adsorbed

Yes

Conjugation

HRP

Excitation/Emission

Applications

IM, IHC-Fr, ICC, ELISA, Dot, WB, IHC-P

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit and Rat Serum Proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Dot": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IM": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "ICC": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/500 - 1/2500", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000 - 1/10000", "notes":"" }, "ELISA": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/170000", "notes":"" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Storage buffer

Preservative: 0.01% Gentamicin sulphate Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Goat IgG

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Journal of proteomics 270:104739 PubMed36174954

2022

Proteomic changes associated with maternal dietary low ω6:ω3 ratio in piglets supplemented with seaweed Part II: Ileum proteomes.

Applications

Unspecified application

Species

Unspecified reactive species

Thi Xuan Nguyen,Alessandro Agazzi,Suzanne McGill,Stefan Weidt,Quang Hanh Han,Andrea Gelemanović,Mark McLaughlin,Giovanni Savoini,Peter David Eckersall,Richard Burchmore

Molecular pharmaceutics 19:2175-2182 PubMed35642083

2022

Comparison of DLin-MC3-DMA and ALC-0315 for siRNA Delivery to Hepatocytes and Hepatic Stellate Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Ferraresso,Amy W Strilchuk,Lih Jiin Juang,Lauren G Poole,James P Luyendyk,Christian J Kastrup

Tissue engineering and regenerative medicine 19:403-415 PubMed35122584

2022

Application of Bone Marrow Mesenchymal Stem Cells Effectively Eliminates Endotoxemia to Protect Rat from Acute Liver Failure Induced by Thioacetamide.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Jiang,Geng Xia,Bo Yang,Hong-Wei Zhang,Yue-Shan Yin,Cheng-Wei Tang,Jin-Hui Yang

Molecular medicine reports 23: PubMed33537819

2021

CDK6 is stimulated by hyperthermia and protects gastric cancer cells from hyperthermia‑induced damage.

Applications

Unspecified application

Species

Unspecified reactive species

Guanghui Liu,Hongchao Zhao,Qingqing Ding,Haohao Li,Tao Liu,Hongwei Yang,Yuanhua Liu

Experimental and therapeutic medicine 20:854-859 PubMed32742328

2020

Ameliorating effect of quercetin on epilepsy by inhibition of inflammation in glial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Dongmei Wu,Zihui Zheng,Shaohua Fan,Xin Wen,Xinrui Han,Shan Wang,Yongjian Wang,Zifeng Zhang,Qun Shan,Mengqiu Li,Bin Hu,Yuanlin Zheng,Jun Lu

Experimental and therapeutic medicine 18:4313-4321 PubMed31777538

2019

Valsartan inhibits RhoA-ROCK2-MYL pathway in rat model of alcoholic cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Luyifei Li,Ling Jing,Jiyi Zhao,Jiachen Lv,Wen Yang,Weimin Li,Lijun Zhou

Oncology 97:26-37 PubMed31071716

2019

Tropomyosin-Related Kinase Receptor and Neurotrophin Expression in Cutaneous Melanoma Is Associated with a Poor Prognosis and Decreased Survival.

Applications

Unspecified application

Species

Unspecified reactive species

Luís Carlos Moreira Antunes,André Cartell,Caroline Brunetto de Farias,Renato Marchiori Bakos,Rafael Roesler,Gilberto Schwartsmann

Cell death & disease 8:e2842 PubMed28569781

2017

Mitochondrial AKAP1 supports mTOR pathway and tumor growth.

Applications

IHC

Species

Unspecified reactive species

Laura Rinaldi,Maria Sepe,Rossella Delle Donne,Kristel Conte,Antonietta Arcella,Domenica Borzacchiello,Stefano Amente,Fernanda De Vita,Monia Porpora,Corrado Garbi,Maria A Oliva,Claudio Procaccini,Deriggio Faicchia,Giuseppe Matarese,Federica Zito Marino,Gaetano Rocco,Sara Pignatiello,Renato Franco,Luigi Insabato,Barbara Majello,Antonio Feliciello

Journal of neuroinflammation 14:59 PubMed28320424

2017

The Alzheimer's disease risk factors apolipoprotein E and TREM2 are linked in a receptor signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Charlotte Jendresen,Vibeke Årskog,Michael R Daws,Lars N G Nilsson

Proceedings of the National Academy of Sciences of 114:E337-E346 PubMed28049839

2017

GAS6 is a key homeostatic immunological regulator of host-commensal interactions in the oral mucosa.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Nassar,Yaara Tabib,Tal Capucha,Gabriel Mizraji,Tsipora Nir,Meirav Pevsner-Fischer,Gili Zilberman-Schapira,Oded Heyman,Gabriel Nussbaum,Herve Bercovier,Asaf Wilensky,Eran Elinav,Tal Burstyn-Cohen,Avi-Hai Hovav

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey Anti-Goat IgG H&L (HRP) preadsorbed

Western blot - Donkey Anti-Goat IgG H&L (HRP) preadsorbed (AB7125)

Western blot - Donkey Anti-Goat IgG H&L (HRP) preadsorbed (AB7125)

Western blot - Donkey Anti-Goat IgG H&L (HRP) preadsorbed (AB7125)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (15)

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