AB97120

Donkey Anti-Goat IgG H&L (HRP) preadsorbed

Name: Donkey Anti-Goat IgG (HRP) preadsorbed secondary antibody (ab97120) | Abcam
Brand: Abcam Limited
SKU: AB97120
Price: 295 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(25 Publications)

Suitable for ICC, IHC-P, ELISA, WB. Ideal for western blot. Preadsorbed to minimise non-specific binding and high background staining. Cited in 25 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

Key facts

Host species

Donkey

Target species

Goat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Rabbit, Chicken, Human, Pig

Pre-adsorbed

Yes

Conjugation

HRP

Excitation/Emission

Applications

ICC, ELISA, WB, IHC-P

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to chicken, horse, human, mouse, pig, rabbit and rat was detected.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/5000", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/100000", "notes":"(Primary)" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/5000 - 1/50000", "notes":"Colorimetric 1/5000 - 1/30000; Chemiluminescent 1/10000 - 1/50000" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using chicken, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.05% CMIT/MIT based preservative Constituents: 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Goat IgG

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Publications (25)

Recent publications for all applications. Explore the full list and refine your search

Nature 611:585-593 PubMed36352225

2022

Parenchymal border macrophages regulate the flow dynamics of the cerebrospinal fluid.

Applications

Unspecified application

Species

Unspecified reactive species

Antoine Drieu,Siling Du,Steffen E Storck,Justin Rustenhoven,Zachary Papadopoulos,Taitea Dykstra,Fenghe Zhong,Kyungdeok Kim,Susan Blackburn,Tornike Mamuladze,Oscar Harari,Celeste M Karch,Randall J Bateman,Richard Perrin,Martin Farlow,Jasmeer Chhatwal,Song Hu,Gwendalyn J Randolph,Igor Smirnov,Jonathan Kipnis

International journal of molecular sciences 22: PubMed34360581

2021

The Slow-Releasing and Mitochondria-Targeted Hydrogen Sulfide (HS) Delivery Molecule AP39 Induces Brain Tolerance to Ischemia.

Applications

Unspecified application

Species

Unspecified reactive species

Bartosz Pomierny,Weronika Krzyżanowska,Jakub Jurczyk,Alicja Skórkowska,Beata Strach,Małgorzata Szafarz,Katarzyna Przejczowska-Pomierny,Roberta Torregrossa,Matthew Whiteman,Monika Marcinkowska,Joanna Pera,Bogusława Budziszewska

Scientific reports 11:11185 PubMed34045646

2021

Small molecule inhibitors of the mitochondrial ClpXP protease possess cytostatic potential and re-sensitize chemo-resistant cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Martina Meßner,Melanie M Mandl,Mathias W Hackl,Till Reinhardt,Maximilian A Ardelt,Karolina Szczepanowska,Julian E Frädrich,Jens Waschke,Irmela Jeremias,Anja Fux,Matthias Stahl,Angelika M Vollmar,Stephan A Sieber,Johanna Pachmayr

Gastroenterology 160:1224-1239.e30 PubMed33197448

2020

The Balance of Stromal BMP Signaling Mediated by GREM1 and ISLR Drives Colorectal Carcinogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroki Kobayashi,Krystyna A Gieniec,Josephine A Wright,Tongtong Wang,Naoya Asai,Yasuyuki Mizutani,Tadashi Lida,Ryota Ando,Nobumi Suzuki,Tamsin R M Lannagan,Jia Q Ng,Akitoshi Hara,Yukihiro Shiraki,Shinji Mii,Mari Ichinose,Laura Vrbanac,Matthew J Lawrence,Tarik Sammour,Kay Uehara,Gareth Davies,Leszek Lisowski,Ian E Alexander,Yoku Hayakawa,Lisa M Butler,Andrew C W Zannettino,M Omar Din,Jeff Hasty,Alastair D Burt,Simon J Leedham,Anil K Rustgi,Siddhartha Mukherjee,Timothy C Wang,Atsushi Enomoto,Masahide Takahashi,Daniel L Worthley,Susan L Woods

Journal of the American Heart Association 9:e017094 PubMed33190596

2020

Extreme Diversity of the Human Vascular Mesenchymal Cell Landscape.

Applications

Unspecified application

Species

Unspecified reactive species

Laura E Bruijn,Brendy E W M van den Akker,Connie M van Rhijn,Jaap F Hamming,Jan H N Lindeman

Life (Basel, Switzerland) 10: PubMed33147716

2020

Cancer Stem Cells in Head and Neck Metastatic Malignant Melanoma Express Components of the Renin-Angiotensin System.

Applications

Unspecified application

Species

Unspecified reactive species

Sam Siljee,Tessa Pilkington,Helen D Brasch,Nicholas Bockett,Josie Patel,Erin Paterson,Paul F Davis,Swee T Tan

Oncology letters 20:1256-1262 PubMed32724366

2020

Function of miR-200a in proliferation and apoptosis of non-small cell lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Huang,Ting Bao,Zhenzhen Li,Guiyi Ji,Li Zhang

Nature communications 10:5657 PubMed31827083

2019

DNA methylation directs microRNA biogenesis in mammalian cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ohad Glaich,Shivang Parikh,Rachel E Bell,Keren Mekahel,Maya Donyo,Yodfat Leader,Ronna Shayevitch,Danna Sheinboim,Sivan Yannai,Dror Hollander,Ze'ev Melamed,Galit Lev-Maor,Gil Ast,Carmit Levy

Molecular medicine reports 20:3793-3801 PubMed31485626

2019

Overexpression of PTEN may increase the effect of pemetrexed on A549 cells via inhibition of the PI3K/AKT/mTOR pathway and carbohydrate metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Li,Junkai Zhang,Ya Su,Yiling Hou,Zhenguo Wang,Lin Zhao,Shengkai Sun,Hao Fu

Frontiers in surgery 6:13 PubMed31024924

2019

Expression of Components of the Renin-Angiotensin System in Pyogenic Granuloma.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica C Papali'i-Curtin,Helen D Brasch,Bede van Schaijik,Jennifer de Jongh,Reginald W Marsh,Swee T Tan,Tinte Itinteang

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Donkey Anti-Goat IgG H&L (HRP) preadsorbed

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (25)

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Primary Antibodies

Primary Antibodies

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Product promise