Suitable for IHC-Fr, IHC-P, Flow Cyt, IM, ICC/IF, Dot. Preadsorbed to minimise non-specific binding and high background staining. Cited in 9 publications.
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- Viruses 15:2023SARS-CoV-2 Aerosol and Intranasal Exposure Models in Ferrets.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElizabeth E Zumbrun,Samantha E Zak,Eric D Lee,Philip A Bowling,Sara I Ruiz,Xiankun Zeng,Jeffrey W Koehler,Korey L Delp,Russel R Bakken,Shannon S Hentschel,Holly A Bloomfield,Keersten M Ricks,Tamara L Clements,April M Babka,John M Dye,Andrew S HerbertPubMed 38140582
- Journal of immunology (Baltimore, Md. : 1950) 208:772-7792022A Recombinant Ig Fragment (IgCw-γεκ) Comprising the Cγ-Cε and C Domains Is an Alternative Reagent to Human IgE.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMinjae Kim,Jeonghyun Lee,Juho Choi,Youngsil Seo,Gyeseo Park,Jinah Jeon,Yerin Jeon,Mi-Gi Lee,Myung-Hee KwonPubMed 35022271
- Nature communications 9:41862018Complement receptor CD46 co-stimulates optimal human CD8 T cell effector function via fatty acid metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGiuseppina Arbore et. al.PubMed 30305631
- The Journal of clinical investigation 127:2222-22342017TNF superfamily receptor OX40 triggers invariant NKT cell pyroptosis and liver injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeixiang Lan et. al.PubMed 28436935
- The Journal of clinical investigation 126:4319-43302016Targeting CAG repeat RNAs reduces Huntington's disease phenotype independently of huntingtin levels.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura Rué et. al.PubMed 27721240
- Cancer discovery 6:986-10052016Mechanism, Consequences, and Therapeutic Targeting of Abnormal IL15 Signaling in Cutaneous T-cell Lymphoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnjali Mishra,Krista La Perle,Sonya Kwiatkowski,Laura A Sullivan,Gregory H Sams,Jessica Johns,Douglas P Curphey,Jing Wen,Kathleen McConnell,Jun Qi,Henry Wong,Giandomenico Russo,Jianying Zhang,Guido Marcucci,James E Bradner,Pierluigi Porcu,Michael A CaligiuriPubMed 27422033
- Nature communications 4:29902013Transcription factor IRF4 drives dendritic cells to promote Th2 differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJesse W Williams,Melissa Y Tjota,Bryan S Clay,Bryan Vander Lugt,Hozefa S Bandukwala,Cara L Hrusch,Donna C Decker,Kelly M Blaine,Bethany R Fixsen,Harinder Singh,Roger Sciammas,Anne I SperlingPubMed 24356538
- Cell transplantation 23:959-792013Umbilical cord blood-derived CD34⁺ cells improve outcomes of traumatic brain injury in rats by stimulating angiogenesis and neurogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSheng-Hsien Chen,Jhi-Joung Wang,Chung-Hwan Chen,Hsiu-Kang Chang,Mao-Tsun Lin,Fong-Ming Chang,Chung-Ching ChioPubMed 23582375
- American journal of physiology. Gastrointestinal a 294:G1257-672008CCL25 and CCL28 promote alpha4 beta7-integrin-dependent adhesion of lymphocytes to MAdCAM-1 under shear flow.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlice Miles et. al.PubMed 18308860
Key facts
- Host species
- Donkey
- Target species
- Goat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Hamster, Human
- Conjugation
- PE
- Excitation/Emission
- Ex: 480;565nm, Em: 578nm
- Applications
- IHC-Fr, IHC-P, Flow Cyt, IM, ICC/IF, Dot
- Clonality
- Polyclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application IHC-Fr | Reactivity Expected | Dilution info - | Notes - |
Application IHC-P | Reactivity Expected | Dilution info - | Notes - |
Application Flow Cyt | Reactivity Expected | Dilution info 1/100 - 1/250 | Notes The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. |
Application IM | Reactivity Expected | Dilution info - | Notes As a general guideline, dilutions of 1/50 to 1/200 should be suitable for most applications. |
Application ICC/IF | Reactivity Expected | Dilution info 1/100 - 1/250 | Notes - |
Application Dot | Reactivity Reacts | Dilution info - | Notes - |
Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G
Recommended products
Suitable for IHC-Fr, IHC-P, Flow Cyt, IM, ICC/IF, Dot. Preadsorbed to minimise non-specific binding and high background staining. Cited in 9 publications.
Key facts
- Description
- Donkey Anti-Goat IgG H&L (PE) preadsorbed
- Host species
- Donkey
- Target species
- Goat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Hamster, Human
- Conjugation
- PE
- Excitation/Emission
- Ex: 480;565nm, Em: 578nm
- Applications
- IHC-Fr, IHC-P, Flow Cyt, IM, ICC/IF, Dot
- Clonality
- Polyclonal
- Pre-adsorbed
- Yes
- Specificity
No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c), Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, or Rat Serum Proteins.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Purification technique
- Affinity purification
- Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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4 product images
Verified CustomerFlow Cytometry - Donkey Anti-Goat IgG H&L (PE) preadsorbed (ab7004)
Very bright secondary antibody and PE is one of the best if you have a weak antigen. I used this in a perfect pairing with FITC in a FACS experiment to check pluripotency markers in human embryonic stem cells.
Dot Blot - Donkey Anti-Goat IgG H&L (PE) preadsorbed (ab7004)
Dot Blot of ab7004.Antigen: Goat IgG.
Load: 100 ng, 33.3 ng, 11.1 ng, 3.7 ng, or 1.23 ng as indicated.
Secondary antibody: ab7004 at 1:1,000 for 60 min at RT.
Block: Blocking Buffer for Fluorescent Western Blotting for 60 min at RT.
Dot Blot - Donkey Anti-Goat IgG H&L (PE) preadsorbed (ab7004)
Dot blot analysis of ab7004. Imaged with BioRad ChemiDoc, Rhodamine filter.
All lanes: Dot Blot - Donkey Anti-Goat IgG H&L (PE) preadsorbed (ab7004)
Lane 1: Goat IgG at 100 ng
Lane 2: Goat IgG at 33.3 ng
Lane 3: Goat IgG at 11.1 ng
Lane 4: Goat IgG at 3.7 ng
Lane 5: Goat IgG at 1.23 ng
SecondaryAll lanes: Donkey F(ab')2 Anti-Goat IgG Antibody RPE at 1 µg/mL
Dot Blot - Donkey Anti-Goat IgG H&L (PE) preadsorbed (ab7004)
Dot blot analysis of ab7004.
All lanes: Dot Blot - Donkey Anti-Goat IgG H&L (PE) preadsorbed (ab7004)
Lane 1: Goat IgG at 100 ng
Lane 2: Goat IgG at 33.3 ng
Lane 3: Goat IgG at 11.1 ng
Lane 4: Goat IgG at 3.7 ng
Lane 5: Goat IgG at 1.23 ng
SecondaryAll lanes: F(ab')2 Donkey anti-Goat IgG Phycoerythrin Conjugated Min X Ch, GP, Ham, Hs, Hu, Ms, Rb, & Rt serum proteins antibody at 1/1000 dilution
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