Donkey Anti-Goat IgG H&L (PE) preadsorbed

• Flow Cyt

Unknown

Flow Cytometry - Donkey Anti-Goat IgG H&L (PE) preadsorbed (AB7004)

Very bright secondary antibody and PE is one of the best if you have a weak antigen. I used this in a perfect pairing with FITC in a FACS experiment to check pluripotency markers in human embryonic stem cells.

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• Dot

Supplier Data

Dot Blot - Donkey Anti-Goat IgG H&L (PE) preadsorbed (AB7004)

Dot Blot of ab7004.

Antigen : Goat IgG.

Load : 100 ng, 33.3 ng, 11.1 ng, 3.7 ng, or 1.23 ng as indicated.

Secondary antibody : ab7004 at 1 : 1,000 for 60 min at RT.

Block : Blocking Buffer for Fluorescent Western Blotting for 60 min at RT.

• Dot

Supplier Data

Dot Blot - Donkey Anti-Goat IgG H&L (PE) preadsorbed (AB7004)

Dot blot analysis of ab7004. Imaged with BioRad ChemiDoc, Rhodamine filter.

All lanes:

Dot Blot - Donkey Anti-Goat IgG H&L (PE) preadsorbed (ab7004)

Lane 1:

Goat IgG at 100 ng

Lane 2:

Goat IgG at 33.3 ng

Lane 3:

Goat IgG at 11.1 ng

Lane 4:

Goat IgG at 3.7 ng

Lane 5:

Goat IgG at 1.23 ng

Secondary

All lanes:

Donkey F(ab')2 Anti-Goat IgG Antibody RPE at 1 µg/mL

false

• Dot

Supplier Data

Dot Blot - Donkey Anti-Goat IgG H&L (PE) preadsorbed (AB7004)

Dot blot analysis of ab7004.

All lanes:

Dot Blot - Donkey Anti-Goat IgG H&L (PE) preadsorbed (ab7004)

Lane 1:

Goat IgG at 100 ng

Lane 2:

Goat IgG at 33.3 ng

Lane 3:

Goat IgG at 11.1 ng

Lane 4:

Goat IgG at 3.7 ng

Lane 5:

Goat IgG at 1.23 ng

Secondary

All lanes:

F(ab')2 Donkey anti-Goat IgG Phycoerythrin Conjugated Min X Ch, GP, Ham, Hs, Hu, Ms, Rb, & Rt serum proteins antibody at 1/1000 dilution

false