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AB7120

Donkey Anti-Goat IgG H&L preadsorbed

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(4 Publications)

Suitable for Conjugation, IHC-Fr, IHC-P, IP, ID, ICC/IF, ELISA, WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 4 publications.
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ELISA - Donkey Anti-Goat IgG H&L preadsorbed (AB7120)
  • ELISA

Supplier Data

ELISA - Donkey Anti-Goat IgG H&L preadsorbed (AB7120)

ELISA results of ab7120 against purified Goat IgG MX7. Each well was coated in duplicate with 1.0 µg of Goat IgG as well as Chicken IgG, Guinea Pig IgG, Hamster IgG, Horse IgG, Mouse IgG, Rabbit IgG, and Rat IgG. The working dilution is 1/56,000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Goat Anti-Donkey IgG Mx7 HRP conjugated, and TMB substrate.

Key facts

Host species

Donkey

Target species

Goat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Hamster

Pre-adsorbed

Yes

Applications

WB, ICC/IF, IHC-Fr, IP, IHC-P, ID, Conjugation, ELISA

applications

Clonality

Polyclonal

Specificity

No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit or Rat Serum Proteins. Specificity was confirmed using ELISA minimal cross reactivity against the targets stated above.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Conjugation": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IP": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "ID": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/1000 - 1/5000", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/150000 - 1/250000", "notes":"<p></p>" }, "WB": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/2000 - 1/10000", "notes":"<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

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Target data

See full target information Goat IgG
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Methods in molecular biology (Clifton, N.J.) 2804:127-138 PubMed38753145

2024

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Applications

Unspecified application

Species

Unspecified reactive species

Inês F Pinto,Veronique Chotteau,Aman Russom

ACS sensors 6:842-851 PubMed33724791

2021

Multiplexed Microfluidic Cartridge for At-Line Protein Monitoring in Mammalian Cell Culture Processes for Biopharmaceutical Production.

Applications

Unspecified application

Species

Unspecified reactive species

Inês F Pinto,Ruben R G Soares,Meeri E-L Mäkinen,Veronique Chotteau,Aman Russom

European journal of pharmacology 762:82-8 PubMed26004535

2015

Non-uniform changes in membrane receptors in the rat urinary bladder following outlet obstruction.

Applications

Unspecified application

Species

Unspecified reactive species

Jianwen Zeng,Mari Ekman,Chonghe Jiang,Bengt Uvelius,Karl Swärd

Dalton transactions (Cambridge, England : 2003) 44:4994-5003 PubMed25612290

2015

Terbium-based time-gated Förster resonance energy transfer imaging for evaluating protein-protein interactions on cell membranes.

Applications

Unspecified application

Species

Unspecified reactive species

Stina Lindén,Manish Kumar Singh,K David Wegner,Marie Regairaz,François Dautry,François Treussart,Niko Hildebrandt
View all publications
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