Suitable for Conjugation, IHC-Fr, IHC-P, IP, ID, ICC/IF, ELISA, WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 4 publications.
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- Methods in molecular biology (Clifton, N.J.) 2804:127-1382024Microfluidic Cartridge for Bead-Based Affinity Assays.Applications:Unspecified applicationReactive species:Unspecified reactive speciesInês F Pinto,Veronique Chotteau,Aman RussomPubMed 38753145
- ACS sensors 6:842-8512021Multiplexed Microfluidic Cartridge for At-Line Protein Monitoring in Mammalian Cell Culture Processes for Biopharmaceutical Production.Applications:Unspecified applicationReactive species:Unspecified reactive speciesInês F Pinto,Ruben R G Soares,Meeri E-L Mäkinen,Veronique Chotteau,Aman RussomPubMed 33724791
- European journal of pharmacology 762:82-82015Non-uniform changes in membrane receptors in the rat urinary bladder following outlet obstruction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianwen Zeng et. al.PubMed 26004535
- Dalton transactions (Cambridge, England : 2003) 44:4994-50032015Terbium-based time-gated Förster resonance energy transfer imaging for evaluating protein-protein interactions on cell membranes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStina Lindén,Manish Kumar Singh,K David Wegner,Marie Regairaz,François Dautry,François Treussart,Niko HildebrandtPubMed 25612290
Key facts
- Host species
- Donkey
- Target species
- Goat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Hamster
- Applications
- Conjugation, IHC-Fr, IHC-P, IP, ID, ICC/IF, ELISA, WB
- Clonality
- Polyclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Conjugation | Reactivity Expected | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Expected | Dilution info - | Notes - |
Application IHC-P | Reactivity Expected | Dilution info - | Notes - |
Application IP | Reactivity Expected | Dilution info - | Notes - |
Application ID | Reactivity Expected | Dilution info - | Notes - |
Application ICC/IF | Reactivity Expected | Dilution info 1/1000 - 1/5000 | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/150000 - 1/250000 | Notes - |
Application WB | Reactivity Expected | Dilution info 1/2000 - 1/10000 | Notes - |
Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G
Recommended products
Suitable for Conjugation, IHC-Fr, IHC-P, IP, ID, ICC/IF, ELISA, WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 4 publications.
Key facts
- Description
- Donkey Anti-Goat IgG H&L preadsorbed
- Host species
- Donkey
- Target species
- Goat
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Hamster
- Applications
- Conjugation, IHC-Fr, IHC-P, IP, ID, ICC/IF, ELISA, WB
- Clonality
- Polyclonal
- Pre-adsorbed
- Yes
- Specificity
No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit or Rat Serum Proteins. Specificity was confirmed using ELISA minimal cross reactivity against the targets stated above.
- Concentration
Properties
- Form
- Liquid
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Purification technique
- Affinity purification
- Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
ELISA - Donkey Anti-Goat IgG H&L preadsorbed (ab7120)
ELISA results of ab7120 against purified Goat IgG MX7. Each well was coated in duplicate with 1.0 µg of Goat IgG as well as Chicken IgG, Guinea Pig IgG, Hamster IgG, Horse IgG, Mouse IgG, Rabbit IgG, and Rat IgG. The working dilution is 1/56,000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Goat Anti-Donkey IgG Mx7 HRP conjugated, and TMB substrate.
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