AB175658

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 405)

Name: Donkey Anti Mouse (IgG) secondary antibody Alexa Fluor® 405 (ab175658) | Abcam
Brand: Abcam Limited
SKU: AB175658
Price: 120 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(49 Publications)

Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Cited in 49 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-4, Ighg1

2 Images

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 405) (AB175658)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 405) (AB175658)

ICC/IF image of ab7291 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes, blocked with Image-iT® FX Signal Enhancer at room temperature for 30 min and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1μg/ml) overnight at +4°C. The secondary antibody (blue) was ab175658 Alexa Fluor^® 405 donkey anti-mouse IgG (H+L) used at 2μg/ml for 1h. DRAQ5 (ab108410) was used to stain the cell nuclei (red) at a concentration of 1.25μM

The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

Alexa Fluor® - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 405) (AB175658)

Alexa Fluor®

Unknown

Alexa Fluor® - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 405) (AB175658)

Emmission spectra of the Alexa Fluor^® conjugated secondary antibodies.

Key facts

Host species

Donkey

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Alexa Fluor® 405

Excitation/Emission

Ex: 401nm, Em: 421nm

Applications

IHC-Fr, Flow Cyt, ELISA, IHC-P, ICC/IF

applications

Clonality

Polyclonal

Isotype

IgG

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor® 405 dye (see difference below) Ex max: Alexa Fluor® 405 = 402nm / DAPI = 359nm Em max: Alexa Fluor® 405 = 421nm / DAPI = 461nm" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"" } } }

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Product details

We recommend storage time at 4°C should be minimal, since this may affect the signal strength.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ighg1

Additional targets

Ighg1

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Publications (49)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:24804 PubMed40640441

2025

Local microglial activation induced and labeled in the retina in a novel subretinal hemorrhage mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Boglárka Balogh,Marietta Zille,Gergely Szarka,Loretta Péntek,Anett Futácsi,Béla Völgyi,Tamás Kovács-Öller

Nature 641:1309-1318 PubMed40307557

2025

Cell cycle duration determines oncogenic transformation capacity.

Applications

Unspecified application

Species

Unspecified reactive species

Danian Chen,Suying Lu,Katherine Huang,Joel D Pearson,Marek Pacal,Phillipos Peidis,Sean McCurdy,Tao Yu,Monika Sangwan,Angela Nguyen,Philippe P Monnier,Daniel Schramek,Liang Zhu,David Santamaria,Mariano Barbacid,Nagako Akeno,Kathryn A Wikenheiser-Brokamp,Rod Bremner

iScience 27:111027 PubMed39435149

2024

TLR2/NF-κB signaling in macrophage/microglia mediated COVID-pain induced by SARS-CoV-2 envelope protein.

Applications

Unspecified application

Species

Unspecified reactive species

Huan Cui,Fengrun Sun,Ning Yu,Yan Cao,Xue Wang,Di Zhang,Zhen Chen,Naili Wang,Bo Yuan,Penghao Liu,Wanru Duan,Wenying Qiu,Xiangsha Yin,Chao Ma

Nature communications 15:6282 PubMed39060233

2024

Apelin modulates inflammation and leukocyte recruitment in experimental autoimmune encephalomyelitis.

Applications

Unspecified application

Species

Unspecified reactive species

Hongryeol Park,Jian Song,Hyun-Woo Jeong,Max L B Grönloh,Bong Ihn Koh,Esther Bovay,Kee-Pyo Kim,Luisa Klotz,Patricia A Thistlethwaite,Jaap D van Buul,Lydia Sorokin,Ralf H Adams

Autophagy 20:2529-2539 PubMed38869076

2024

CCDC50 mediates the clearance of protein aggregates to prevent cellular proteotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Ye,Penghui Jia,Jiafan Miao,Yicheng Wang,Zibo Li,Yuxin Lin,Miao He,Shurui Liu,Bi-Rong Zheng,Junyu Wu,Ji'an Pan,Chun-Mei Li,Panpan Hou,Deyin Guo

Journal of cell science 137: PubMed38757366

2024

The Drosophila Nesprin-1 homolog MSP300 is required for muscle autophagy and proteostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin van der Graaf,Saurabh Srivastav,Rajkishor Nishad,Michael Stern,James A McNew

The Journal of neuroscience : the official journal of the Society for Neuroscience 44: PubMed38154957

2023

Glutamate Spillover Dynamically Strengthens Gabaergic Synaptic Inhibition of the Hypothalamic Paraventricular Nucleus.

Applications

Unspecified application

Species

Unspecified reactive species

Junya Yamaguchi,Mary Ann Andrade,Tamara T Truong,Glenn M Toney

Molecular & cellular proteomics : MCP 23:100704 PubMed38128648

2023

Proteomic Analysis Reveals the Composition of Glutamatergic Organelles of Auditory Inner Hair Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Andreia P Cepeda,Momchil Ninov,Jakob Neef,Iwan Parfentev,Kathrin Kusch,Ellen Reisinger,Reinhard Jahn,Tobias Moser,Henning Urlaub

Traffic (Copenhagen, Denmark) 24:587-609 PubMed37846526

2023

Copper-independent lysosomal localisation of the Wilson disease protein ATP7B.

Applications

Unspecified application

Species

Unspecified reactive species

Saptarshi Maji,Marinella Pirozzi, Ruturaj,Raviranjan Pandey,Tamal Ghosh,Santanu Das,Arnab Gupta

Aging and disease 14:1870-1886 PubMed37196130

2023

Optogenetic Activation of Astrocytes Reduces Blood-Brain Barrier Disruption via IL-10 In Stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Suo,Lidong Deng,Tingting Chen,Shengju Wu,Lin Qi,Ze Liu,Tingting He,Heng-Li Tian,Wanlu Li,Yaohui Tang,Guo-Yuan Yang,Zhijun Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 405)

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 405) (AB175658)

Alexa Fluor® - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 405) (AB175658)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Additional targets

Publications (49)

Product promise