AB175659

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 405) preadsorbed

Name: Donkey Anti Mouse (IgG) secondary antibody preadsorbed Alexa Fluor® 405
Brand: Abcam Limited
SKU: AB175659

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(8 Publications)

Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 8 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-4, Ighg1

2 Images

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 405) preadsorbed (AB175659)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 405) preadsorbed (AB175659)

ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the primary antibody (ab7291, 1μg/ml) overnight at +4°C. The secondary antibody (blue) was ab175659, Donkey Anti-Mouse IgG H&L (Alexa Fluor® 405) preadsorbed, used at 1μg/ml for 1h at room temperature. DRAQ5™ (ab108410) was used to stain the cell nuclei (red) at a dilution of 1/4000.

The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

Alexa Fluor® - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 405) preadsorbed (AB175659)

Alexa Fluor®

Unknown

Alexa Fluor® - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 405) preadsorbed (AB175659)

Key facts

Host species

Donkey

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Rat, Sheep, Rabbit, Goat, Chicken, Cow, Human

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 405

Excitation/Emission

Ex: 401nm, Em: 421nm

Applications

IHC-Fr, Flow Cyt, ELISA, IHC-P, ICC/IF

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, human, rabbit, rat and sheep IgG was detected. This antibody may cross react with IgG from other species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor® 405 dye (see difference below) Ex max: Alexa Fluor® 405 = 402nm / DAPI = 359nm Em max: Alexa Fluor® 405 = 421nm / DAPI = 461nm" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"" } } }

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Product details

We recommend storage time at 4°C should be minimal, since this may affect the signal strength.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using bovine, chicken, goat, human, rabbit, rat and sheep immunosorbents to remove cross reactive antibodies. The antibody to mouse IgG was isolated by affinity chromatography using antigen coupled to agarose beads.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ighg1

Additional targets

Ighg1

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in neuroscience 17:1204233 PubMed37650102

2023

New insight on the enteric cholinergic innervation of the pig colon by central and peripheral nervous systems: reduction by repeated loperamide administration.

Applications

Unspecified application

Species

Unspecified reactive species

Pu-Qing Yuan,Tao Li,Mulugeta Million,Muriel Larauche,Karim Atmani,Jean-Pierre Bellier,Yvette Taché

Genome medicine 14:19 PubMed35189942

2022

DevKidCC allows for robust classification and direct comparisons of kidney organoid datasets.

Applications

Unspecified application

Species

Unspecified reactive species

Sean B Wilson,Sara E Howden,Jessica M Vanslambrouck,Aude Dorison,Jose Alquicira-Hernandez,Joseph E Powell,Melissa H Little

eLife 10: PubMed34878404

2021

Tightly coupled inhibitory and excitatory functional networks in the developing primary visual cortex.

Applications

Unspecified application

Species

Unspecified reactive species

Haleigh N Mulholland,Bettina Hein,Matthias Kaschube,Gordon B Smith

Neuron 109:2847-2863.e11 PubMed34407390

2021

Proneural genes define ground-state rules to regulate neurogenic patterning and cortical folding.

Applications

Unspecified application

Species

Unspecified reactive species

Sisu Han,Satoshi Okawa,Grey Atteridge Wilkinson,Hussein Ghazale,Lata Adnani,Rajiv Dixit,Ligia Tavares,Imrul Faisal,Matthew J Brooks,Veronique Cortay,Dawn Zinyk,Adam Sivitilli,Saiqun Li,Faizan Malik,Yaroslav Ilnytskyy,Vladimir Espinosa Angarica,Jinghua Gao,Vorapin Chinchalongporn,Ana-Maria Oproescu,Lakshmy Vasan,Yacine Touahri,Luke Ajay David,Eko Raharjo,Jung-Woong Kim,Wei Wu,Waleed Rahmani,Jennifer Ai-Wen Chan,Igor Kovalchuk,Liliana Attisano,Deborah Kurrasch,Colette Dehay,Anand Swaroop,Diogo S Castro,Jeff Biernaskie,Antonio Del Sol,Carol Schuurmans

Journal of the American Society of Nephrology : JASN 32:1697-1712 PubMed33911000

2021

Autonomous Calcium Signaling in Human and Zebrafish Podocytes Controls Kidney Filtration Barrier Morphogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Lydia Djenoune,Ritu Tomar,Aude Dorison,Irene Ghobrial,Heiko Schenk,Jan Hegermann,Lynne Beverly-Staggs,Alejandro Hidalgo-Gonzalez,Melissa H Little,Iain A Drummond

Science advances 7: PubMed33692111

2021

Robust information routing by dorsal subiculum neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Takuma Kitanishi,Ryoko Umaba,Kenji Mizuseki

Neuron 106:816-829.e6 PubMed32229307

2020

All-Electrical Ca-Independent Signal Transduction Mediates Attractive Sodium Taste in Taste Buds.

Applications

Unspecified application

Species

Unspecified reactive species

Kengo Nomura,Miho Nakanishi,Fumiyoshi Ishidate,Kazumi Iwata,Akiyuki Taruno

Brain and behavior 9:e01353 PubMed31271523

2019

Sensory deafferentation modulates and redistributes neurocan in the rat auditory brainstem.

Applications

Unspecified application

Species

Unspecified reactive species

Josef Heusinger,Heika Hildebrandt,Robert-Benjamin Illing

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 405) preadsorbed

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 405) preadsorbed (AB175659)

Alexa Fluor® - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 405) preadsorbed (AB175659)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Alternative Products

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Primary Antibodies

Primary Antibodies

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Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Additional targets

Publications (8)

Product promise