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AB150105

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488)

4

(5 Reviews)

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(451 Publications)

Donkey anti-mouse IgG H&L (Alexa Fluor® 488) is a secondary antibody with a maximum absorption wavelength of 495nm and a maximum emission wavelength of 519nm. Ideal for fluorescent cell and tissue imaging. Suitable for ICC/IF, IHC, flow cytometry and ELISA.

- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 320 publications

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form

2 Images
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150105)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150105)

ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 5μg/ml) overnight at +4°C. The secondary antibody (green) was ab150105 Alexa Fluor® 488 donkey anti-mouse IgG (H+L) used at 2μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

Alexa Fluor® - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150105)
  • Alexa Fluor®

Unknown

Alexa Fluor® - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150105)

Key facts

Host species

Donkey

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Applications

ELISA, Flow Cyt, IHC-P, ICC/IF, IHC-Fr

applications

Clonality

Polyclonal

Isotype

IgG

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1 (Alexa Fluor<sup>®</sup> 488), is suitable for use as an isotype control to complement this secondary antibody.</p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"<p></p>" } } }
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Product details

Fluorochrome chart - a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.

When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers a comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
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Product protocols

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Target data

See full target information Ighg1

Additional targets

Ighg1
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Publications (451)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8678 PubMed41028711

2025

Genetic tuning of retinal ganglion cell subtype identity to drive visual behavior.

Applications

Unspecified application

Species

Unspecified reactive species

Marcos L Aranda,Jacob D Bhoi,Omar A Payán Parra,Seul Ki Lee,Tomoko Yamada,Yue Yang,Tiffany M Schmidt

Cell metabolism 37:1783-1788 PubMed40902582

2025

Reassessment of antibody-based detection of the murine T cell GLP-1 receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Chi Kin Wong,Bernardo Yusta,Jason C L Tong,Johannes Broichhagen,David J Hodson,Daniel J Drucker

Frontiers in neurology 16:1606606 PubMed40895108

2025

Intraventricular continuous BDNF administration ameliorates neuroinflammation and enhances neurogenesis against rodent intracerebral hemorrhage model.

Applications

Unspecified application

Species

Unspecified reactive species

Ting-Chun Lin,Masahito Kawabori,Erika Yoshie,Yo Nakahara,Li-Kai Tsai,Miki Fujimura

Brain sciences 15: PubMed40867154

2025

The Synergistic Effect of Heat Therapy and Electroacupuncture Treatment in Inflammatory Pain Mouse Models.

Applications

Unspecified application

Species

Unspecified reactive species

Boon Khai Teoh,Sharmely Sharon Ballon Romero,Tran Van Bao Quach,Hsin-Yi Chung,Yi-Hung Chen

Stem cell research & therapy 16:443 PubMed40830519

2025

Harnessing TGF-β signaling to improve testicular organoid development from dissociated testicular cells.

Applications

Unspecified application

Species

Unspecified reactive species

Seyedeh-Faezeh Moraveji,Saiedeh Erfanian,Mohammad Hossein Ghanian,Hossein Baharvand

ACS omega 10:34747-34761 PubMed40821548

2025

Exploration of a Class of Aryl Imidazolyl Ureas As Potent Acid Ceramidase Inhibitors for the Treatment of Fibrotic Diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Richard Beresis,Vijaya Prathigudupu,Carson Cable,Amy Yu,Marc Adler,Roopa Ramamoorthi,Seul Ki Yeon,John D Gordan,Sachin Sharma,William F DeGrado,Balyn Zaro,Jennifer Y Chen,Hyunil Jo

Frontiers in oncology 15:1632245 PubMed40772038

2025

Complementary detection strategies for circulating tumor cells in breast cancer: clinical implications of combining immunofluorescence and cytopathological staining.

Applications

Unspecified application

Species

Unspecified reactive species

Tanja Jesenko,Cvetka Grasic Kuhar,Ziva Pisljar,Simona Miceska,Veronika Kloboves-Prevodnik,Maja Cemazar

Frontiers in neuroscience 19:1615208 PubMed40761316

2025

Chemogenetic tools for modulation of spatial learning in dopamine transporter deficient rats.

Applications

Unspecified application

Species

Unspecified reactive species

Arina A Gromova,Taisiia S Shemiakova,Mikhail G Khotin,Evgeniia N Petrunina,Anastasia D Belskaya,Raul R Gainetdinov,Natalia P Kurzina,Anna B Volnova

The Journal of cell biology 224: PubMed40608049

2025

Sphingolipid synthesis maintains nuclear membrane integrity and genome stability during cell division.

Applications

Unspecified application

Species

Unspecified reactive species

Sunyoung Hwang,William Russo,Jaylah Cormier,Jillian Johnson,Sara Martin,Marica Rosaria Ippolito,Sara Cordone,Rui Li,Lihua Julie Zhu,Stefano Santaguida,Eduardo M Torres

Cell communication and signaling : CCS 23:319 PubMed40598224

2025

A novel mechanism of sperm midpiece epididymal maturation and the role of CCDC112 in sperm midpiece formation and establishing an optimal flagella waveform.

Applications

Unspecified application

Species

Unspecified reactive species

Maddison L Graffeo,Joseph Nguyen,Farin Yazdan Parast,Jessica E M Dunleavy,Denis Korneev,Hongyi Yang,Hidenobu Okuda,Anne E O'Connor,Donald F Conrad,Reza Nosrati,Brendan J Houston,Moira K O'Bryan
View all publications
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