Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) is a preadsorbed secondary antibody with a maximum absorption wavelength of 495nm and a maximum emission wavelength of 519nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.
- Preadsorbed to prevent cross-reactivity and reduce background staining
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 80 publications
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- eLife 12:2023CCR1 mediates Müller cell activation and photoreceptor cell death in macular and retinal degeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah Elbaz-Hayoun,Batya Rinsky,Shira Hagbi-Levi,Michelle Grunin,Itay ChowersPubMed 37903056
- Renal failure 45:22204182023Macrophage polarization induces endothelium-to-myofibroblast transition in chronic allograft dysfunction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZeping Gui,Xiang Zhang,Qianguang Han,Zhou Hang,Ruoyun Tan,Min Gu,Zijie WangPubMed 37288756
- Frontiers in cell and developmental biology 11:11736882023Balancing WNT signalling in early forebrain development: The role of LRP4 as a modulator of LRP6 function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuang Geng,Fabian Paul,Izabela Kowalczyk,Sandra Raimundo,Anje Sporbert,Tamrat Meshka Mamo,Annette HammesPubMed 37091972
- Journal of nuclear medicine : official publication, Society of Nuclear Medicine 64:1056-10612023Addition of Peptide Receptor Radiotherapy to Immune Checkpoint Inhibition Therapy Improves Outcomes in Neuroendocrine Tumors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShadi A Esfahani,Carolina De Aguiar Ferreira,Priska Summer,Umar Mahmood,Pedram HeidariPubMed 37024303
- Molecular therapy. Methods & clinical development 29:350-3632023Efficient autocrine and paracrine signaling explain the osteogenic superiority of transgenic BMP-2 over rhBMP-2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAysegul Atasoy-Zeybek,Michael J Coenen,Gresin P Hawse,Delphine Logeart-Avramoglou,Christopher H Evans,Rodolfo E De La VegaPubMed 37214314
- Stem cell reports 18:817-8282023Differentiation of uniparental human embryonic stem cells into granulosa cells reveals a paternal contribution to gonadal development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGal Keshet,Shiran Bar,Roni Sarel-Gallily,Ofra Yanuka,Nissim Benvenisty,Talia Eldar-GevaPubMed 37001516
- Neural regeneration research 18:2011-20182023Heat shock factor 1 promotes neurite outgrowth and suppresses inflammation in the severed spinal cord of geckos.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBing-Qiang He,Ai-Cheng Li,Yu-Xuan Hou,Hui Li,Xing-Yuan Zhang,Hui-Fei Hao,Hong-Hua Song,Ri-Xin Cai,Ying-Jie Wang,Yue Zhou,Yong-Jun WangPubMed 36926727
- Nucleic acids research 51:3375-33902023Effective extraction of polyribosomes exposes gene expression strategies in primary astrocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShir Mandelboum,Melisa Herrero,Andrea Atzmon,Marcelo Ehrlich,Orna Elroy-SteinPubMed 36881761
- Frontiers in immunology 14:11424922023Human intestinal epithelial cells can internalize luminal fungi LC3-associated phagocytosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarit Cohen-Kedar,Efrat Shaham Barda,Keren Masha Rabinowitz,Danielle Keizer,Hanan Abu-Taha,Shoshana Schwartz,Kawsar Kaboub,Liran Baram,Eran Sadot,Ian White,Nir Wasserberg,Meirav Wolff-Bar,Adva Levy-Barda,Iris DotanPubMed 36969163
- Biology of sex differences 14:82023Investigation of autism-related transcription factors underlying sex differences in the effects of bisphenol A on transcriptome profiles and synaptogenesis in the offspring hippocampus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSurangrat Thongkorn,Songphon Kanlayaprasit,Kasidit Kasitipradit,Pattanachat Lertpeerapan,Pawinee Panjabud,Valerie W Hu,Depicha Jindatip,Tewarit SarachanaPubMed 36803626
Key facts
- Host species
- Donkey
- Target species
- Mouse
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Rat, Sheep, Rabbit, Goat, Chicken, Cow, Human
- Conjugation
- Alexa Fluor® 488
- Excitation/Emission
- Ex: 495nm, Em: 519nm
- Applications
- ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF
- Clonality
- Polyclonal
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000 | Notes ab170190 - Mouse monoclonal IgG1 (Alexa Fluor® 488), is suitable for use as an isotype control to complement this secondary antibody. |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
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Target data
Additional Targets
Alternative names
Igh-4, Ighg1, Ig gamma-1 chain C region secreted form
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Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) is a preadsorbed secondary antibody with a maximum absorption wavelength of 495nm and a maximum emission wavelength of 519nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.
- Preadsorbed to prevent cross-reactivity and reduce background staining
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 80 publications
Key facts
- Description
- Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed
- Host species
- Donkey
- Target species
- Mouse
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Rat, Sheep, Rabbit, Goat, Chicken, Cow, Human
- Conjugation
- Alexa Fluor® 488
- Excitation/Emission
- Ex: 495nm, Em: 519nm
- Applications
- ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF
- Clonality
- Polyclonal
- Pre-adsorbed
- Yes
- Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, human, rabbit, rat and sheep IgG was detected. This antibody may cross react with IgG from other species.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
Antiserum was cross adsorbed using bovine, chicken, goat, human, rabbit, rat and sheep immunosorbents to remove cross reactive antibodies. The antibody to mouse IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
This antibody reacts with the heavy and light chains of Mouse IgG
Fluorochrome chart - a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.
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Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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4 product images
Flow Cytometry - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150109)
Overlay histogram showing Jurkat cells stained with Anti-CD3 antibody [MEM-57] ab8090 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal donkey serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-CD3 antibody [MEM-57] ab8090, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody Donkey anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150109) was used at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control ab91361, 0.01μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150109)
ICC/IF image of Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab150109 Alexa Fluor® 488 donkey anti-mouse IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Alexa Fluor® - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150109)
Emmission spectra of the Alexa Fluor® conjugatedsecondary antibodies.
This image is courtesy of an anonymous customer reviewImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150109)
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