AB150109

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed

Name: Donkey Anti-Mouse IgG (Alexa Fluor® 488) preadsorbed secondary antibody (ab150109) | Abcam
Brand: Abcam Limited
SKU: AB150109
Price: 275 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(123 Publications)

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) is a preadsorbed secondary antibody with a maximum absorption wavelength of 495nm and a maximum emission wavelength of 519nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.

- Preadsorbed to prevent cross-reactivity and reduce background staining
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 80 publications

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-4, Ighg1

4 Images

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (AB150109)

ICC/IF

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Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (AB150109)

ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab150109 Alexa Fluor® 488 donkey anti-mouse IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Flow Cytometry - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (AB150109)

Flow Cyt

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Flow Cytometry - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (AB150109)

Overlay histogram showing Jurkat cells stained with ab8090 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal donkey serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab8090, 0.01μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody Donkey anti-mouse IgG H&L (Alexa Fluor^® 488, pre-adsorbed) (ab150109) was used at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.01μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (AB150109)

IHC-P

AbReview73774****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (AB150109)

This image is courtesy of an anonymous Abreview

Alexa Fluor® - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (AB150109)

Alexa Fluor®

Unknown

Alexa Fluor® - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (AB150109)

Emmission spectra of the Alexa Fluor^® conjugated secondary antibodies.

Key facts

Host species

Donkey

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Rat, Sheep, Rabbit, Goat, Chicken, Cow, Human

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Applications

ELISA, IHC-P, IHC-Fr, Flow Cyt, ICC/IF

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, human, rabbit, rat and sheep IgG was detected. This antibody may cross react with IgG from other species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1 (Alexa Fluor® 488), is suitable for use as an isotype control to complement this secondary antibody." }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"" } } }

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Product details

This antibody reacts with the heavy and light chains of Mouse IgG

Fluorochrome chart- a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.

When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using bovine, chicken, goat, human, rabbit, rat and sheep immunosorbents to remove cross reactive antibodies. The antibody to mouse IgG was isolated by affinity chromatography using antigen coupled to agarose beads.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ighg1

Additional targets

Ighg1

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Publications (123)

Recent publications for all applications. Explore the full list and refine your search

eLife 12: PubMed41025959

2025

Matrix-associated extracellular vesicles modulate human smooth muscle cell adhesion and directionality by presenting collagen VI.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander N Kapustin,Sofia Serena Tsakali,Meredith Whitehead,George Chennell,Meng-Ying Wu,Chris Molenaar,Anton Kutikhin,Yimeng Chen,Sadia Ahmad,Leo Bogdanov,Maxim Sinitsky,Kseniya Rubina,Aled Clayton,Frederik J Verweij,Dirk Michiel Pegtel,Simona Zingaro,Arseniy Lobov,Bozhana Zainullina,Dylan Owen,Maddy Parsons,Richard E Cheney,Derek T Warren,Martin James Humphries,Thomas Iskratsch,Mark Holt,Catherine M Shanahan

Nucleic acids research 53: PubMed41002028

2025

RNF4 and USP7 coordinate spatial regulation of SLX4 stability within the PML nuclear bodies.

Applications

Unspecified application

Species

Unspecified reactive species

Eunyoung Jung,Myung-Jin Kim,Orlando D Schärer,Yonghwan Kim

Molecular reproduction and development 92:e70049 PubMed40838423

2025

Three-Dimensional Magnetic Bioprinting Spheroids as an In Vitro Model to Study the Oviductal Physiology.

Applications

Unspecified application

Species

Unspecified reactive species

Patricia Kubo Fontes,Ana Beatriz Florencio da Silva,Ana Beatriz Dos Reis Bartoli,Thays Antunes,Arnaldo Rodrigues Dos Santos Júnior,Marcella Pecora Milazzotto

Scientific reports 15:25996 PubMed40676062

2025

Prenatal exposure to bisphenol A disrupts RNA splicing in the prefrontal cortex and promotes behaviors related to autism in offspring.

Applications

Unspecified application

Species

Unspecified reactive species

Pawinee Panjabud,Songphon Kanlayaprasit,Surangrat Thongkorn,Kwanjira Songsritaya,Pattanachat Lertpeerapan,Kasidit Kasitipradit,Thanawin Jantheang,Suthathip Sarobol,Thanit Saeliw,Valerie W Hu,Takeshi Imai,Tewarit Sarachana

The Journal of general virology 106: PubMed40668623

2025

Kinesin light chain 1 interacts with NS1 and is a susceptibility factor for dengue virus infection in mosquito cells.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Manuel Castillo,Raymundo Cruz-Pérez,Daniel Talamás-Lara,Juan E Ludert

Biology direct 20:80 PubMed40615904

2025

CAF-derived exosomal miR-196b-5p after androgen deprivation therapy promotes epithelial-mesenchymal transition in prostate cancer cells through HOXC8/NF-κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaodong Song,Tiewen Li,Wenhao Zhou,Chengling Feng,Zeng Zhou,Yuanming Chen,Deng Li,Lei Chen,Jing Zhao,Yu Zhang,Bangmin Han

Molecular therapy. Nucleic acids 36:102499 PubMed40171278

2025

SHMT2 is essential for mammalian preimplantation embryonic development through biosynthesis of nucleotide metabolites.

Applications

Unspecified application

Species

Unspecified reactive species

Mingze Shi,Yingxue Huai,Tiantian Deng,Chuanxin Zhang,Jinzhu Song,Jiawei Wang,Yiwen Zhang,Zi-Jiang Chen,Han Zhao,Keliang Wu,Boyang Liu

iScience 28:112005 PubMed40092609

2025

Spatiotemporal profiling of protein kinase A activity in spontaneously beating hiPSC-derived cardiac organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Ido Weiser-Bitoun,Savyon Mazgaoker,Rami Eid,Inbar Brosh,Yael Yaniv

Journal of neurochemistry 169:e70004 PubMed39871624

2025

GABA Receptor Modulation of Membrane Excitability in Human Pluripotent Stem Cell-Derived Sensory Neurons by Baclofen and α-Conotoxin Vc1.1.

Applications

Unspecified application

Species

Unspecified reactive species

Mitchell St Clair-Glover,Arsalan Yousuf,Dominic Kaul,Mirella Dottori,David J Adams

Nature cell biology 27:347-359 PubMed39779941

2025

Mapping the developmental trajectory of human astrocytes reveals divergence in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Caitlin Sojka,Hsiao-Lin V Wang,Tarun N Bhatia,Yangping Li,Pankaj Chopra,Anson Sing,Anna Voss,Alexia King,Feng Wang,Kevin Joseph,Vidhya M Ravi,Jeffrey Olson,Kimberly Hoang,Edjah Nduom,Victor G Corces,Bing Yao,Steven A Sloan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (AB150109)

Flow Cytometry - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (AB150109)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (AB150109)

Alexa Fluor® - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (AB150109)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Alternative Products

Secondary Antibodies

Secondary Antibodies

Secondary Antibodies

Secondary Antibodies

Secondary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Additional targets

Publications (123)

Product promise