Donkey Anti-Mouse IgG H&L (Alexa Fluor® 555)
5
(1 Review)
|
(80 Publications)
Donkey Anti-Mouse IgG H&L (Alexa Fluor® 555) is a secondary antibody with a maximum absorption wavelength of 555nm and a maximum emission wavelength of 565nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 50 publications
View Alternative Names
Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-4, Ighg1
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 555) (AB150106)
ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1μg/ml) overnight at +4°C. The secondary antibody (yellow) was ab150106 Alexa Fluor® 555 donkey anti-mouse IgG (H+L) used at 2μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
- Alexa Fluor®
Unknown
Alexa Fluor® - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 555) (AB150106)
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Reactivity data
Product details
This antibody reacts with the heavy and light chains of Mouse IgG
Fluorochrome chart- a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.
When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Publications (80)
Recent publications for all applications. Explore the full list and refine your search
Molecular biomedicine 6:75 PubMed41037185
2025
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Current biology : CB 35:4106-4120.e7 PubMed40749677
2025
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Translational psychiatry 15:126 PubMed40185739
2025
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EMBO reports 26:1792-1815 PubMed39984683
2025
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Advanced biology 9:e2400748 PubMed39957575
2025
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Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2406759 PubMed39840526
2025
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Redox biology 79:103469 PubMed39700693
2024
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Development (Cambridge, England) 151: PubMed39373366
2024
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Frontiers in neuroscience 18:1401571 PubMed39114482
2024
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Cancer innovation 3:e113 PubMed38946933
2024
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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