Donkey Anti-Mouse IgG H&L (Alexa Fluor® 555)
5
(1 Review)
|
(57 Publications)
Donkey Anti-Mouse IgG H&L (Alexa Fluor® 555) is a secondary antibody with a maximum absorption wavelength of 555nm and a maximum emission wavelength of 565nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 50 publications
View Alternative Names
Igh-4, Ighg1, Ig gamma-1 chain C region secreted form
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 555) (AB150106)
ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1μg/ml) overnight at +4°C. The secondary antibody (yellow) was ab150106 Alexa Fluor® 555 donkey anti-mouse IgG (H+L) used at 2μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
- Alexa Fluor®
Unknown
Alexa Fluor® - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 555) (AB150106)
Reactivity data
Product details
This antibody reacts with the heavy and light chains of Mouse IgG
Fluorochrome chart- a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.
When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties and storage information
Form
Purification technique
Purification notes
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Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Publications (57)
Recent publications for all applications. Explore the full list and refine your search
Acta neuropathologica communications 11:206 PubMed38124145
2023
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Scientific reports 13:17874 PubMed37857701
2023
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Acta neuropathologica communications 11:137 PubMed37608352
2023
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iScience 26:106545 PubMed37128547
2023
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Frontiers in endocrinology 14:1139121 PubMed36967803
2023
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Pharmaceutics 15: PubMed36839732
2023
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Scientific reports 12:22641 PubMed36587177
2022
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Development (Cambridge, England) 149: PubMed36278804
2022
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Brain plasticity (Amsterdam, Netherlands) 8:5-18 PubMed36448040
2022
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In vivo (Athens, Greece) 36:2032-2041 PubMed36099107
2022
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