Donkey Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed (AB150110)

ab27591 staining DDX4 / MVH antibody in pig tissue sections (testis, postnatal day 42) by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% 0.1% Triton X-100 in PBS and blocked with 3% BSA for 30 minutes at 20°C. Samples were incubated with primary antibody (1/200 in PBS) for 1 hour at 20°C. ab150110 at a dilution of 1/500 was used as the secondary antibody.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed (AB150110)

ICC/IF image of ab7291 in HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1μg/ml) overnight at +4°C. The secondary antibody ab150110 (shown in yellow) was used at 2μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

• IHC-Fr

AbReview

Immunohistochemistry (Frozen sections) - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed (AB150110)

Postnatal day 42 pig testes were fixed with 4% paraformaldehyde. Tissue was embedded in O.C.T. and frozen. 5 micron sections were cut and transferred to slides. Sections were permeabilized with 0.1% Triton X-100 in PBS, and blocked with 3% BSA in 0.1% Triton X-100 + PBS. Sections were incubated with either (A) no primary antibody or (B) anti-DDX4 (ab27591) for 1 h at RT. Sections were then washed 3X with 0.1% Triton X-100 in PBS and Goat-Anti Mouse 555 (ab150110) applied at a 1/500 dilution. Sections were then mounted after washing 3X with 0.1% Triton X-100 in PBS. Phalloidin (blue), which labels F-actin was used as a counterstain.

This image is courtesy of an Abreview submitted by Bryan Niedenberger

• Alexa Fluor®

Unknown

Alexa Fluor® - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed (AB150110)